Open Access

Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury

  • Authors:
    • Xiaofen Ruan
    • Tiejun Chen
    • Xiaolong Wang
    • Yiping Li
  • View Affiliations

  • Published online on: August 18, 2017     https://doi.org/10.3892/etm.2017.4964
  • Pages: 3523-3532
  • Copyright: © Ruan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Suxiao Jiuxin Pill (SX), a traditional Chinese medicine compound consisting primarily of tetramethylpyrazine and borneol, has been reported to protect against ischemic heart disease. However, the effects of SX on mitochondrial injury and gene expression in various signaling pathways are unclear. The aim of the present study was to investigate the effects of SX on mitochondrial injury and to screen the expression of genes potentially altered by SX using a cell culture model of ischemic injury. Simulated ischemia was established by culturing HL‑1 cardiomyocytes in Dulbecco's modified Eagle medium without glucose or serum in a hypoxic chamber containing 95% N2 and 5% CO2 for 24 h. HL‑1 cardiomyocytes were divided into 3 groups: Control, ischemic injury and ischemic injury + SX (100 µg/ml; n=3 wells/group). Mitochondrial membrane potential was detected by staining with JC‑1 dye. The mRNA expression levels of adenylyl cyclase (Adcy) 1‑9, adrenoceptor β1, Akt1, ATPase Na+/K+ transporting subunit β2, calcium voltage‑gated channel auxiliary subunit α2δ (Cacna2d)2, Cacna2d3, calcium channel voltage‑dependent γ subunit 8, cytochrome C oxidase subunit 6A2 (Cox6a2), fibroblast growth factor receptor (Fgfr) 4, Fgf8, Fgf12, Gnas complex locus, glycogen synthase kinase 3β (Gsk3b), mitogen‑activated protein kinase (Mapk)11‑14, Mapk kinase kinase kinase 1 (Map4k1), Mas1, nitric oxide synthase 3 (Nos3), phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (Pik3ca), phospholipase A2 group 4A, rap guanine nucleotide exchange factor 4 and ryanodine receptor 2 were detected using reverse transcription‑quantitative polymerase chain reaction. The protein expression levels of phosphoinositide 3‑kinase (PI3K), MAS‑1 and phosphorylated‑endothelial NOS were also examined by immunofluorescence staining. The decrease in mitochondrial membrane potential in the cell culture model of ischemic injury (P<0.001) was significantly attenuated by SX treatment (P<0.001). Furthermore, increases in the mRNA expression levels of Adcy2 (P<0.05), 3 (P<0.01) and 8 (P<0.05) in the ischemic injury model were significantly attenuated by SX treatment (P<0.01), and SX treatment significantly decreased the mRNA expression levels of Adcy1 (P<0.01) and 6 (P<0.05) in ischemic cells. Decreases in the mRNA expression levels of Cox6a2 (P<0.001), Gsk3b (P<0.01) and Pik3ca (P<0.001) in the ischemic injury model were also significantly attenuated by SX treatment (P<0.05, P<0.01 and P<0.001, respectively). In addition, the decrease in the protein expression of PI3K (P<0.001) was significantly attenuated by SX treatment (P<0.001). The present findings indicate that SX may protect cardiomyocytes against mitochondrial injury and attenuate alterations in the gene expression of Adcy2, 3 and 8, Cox6a2, Gsk3b and Pik3ca during ischemic injury.

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October-2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Ruan X, Chen T, Wang X and Li Y: Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury. Exp Ther Med 14: 3523-3532, 2017
APA
Ruan, X., Chen, T., Wang, X., & Li, Y. (2017). Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury. Experimental and Therapeutic Medicine, 14, 3523-3532. https://doi.org/10.3892/etm.2017.4964
MLA
Ruan, X., Chen, T., Wang, X., Li, Y."Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury". Experimental and Therapeutic Medicine 14.4 (2017): 3523-3532.
Chicago
Ruan, X., Chen, T., Wang, X., Li, Y."Suxiao Jiuxin Pill protects cardiomyocytes against mitochondrial injury and alters gene expression during ischemic injury". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3523-3532. https://doi.org/10.3892/etm.2017.4964