Open Access

Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration

  • Authors:
    • Yi Zhang
    • Dandan Zhang
    • Wei Wei
    • Bingqiao Shen
    • Yuyao Wang
    • Yingjie Zhang
    • Yidan Zhang
    • Jing Ji
    • Hao Sun
    • Min Luo
    • Ping Gu
  • View Affiliations

  • Published online on: August 22, 2017     https://doi.org/10.3892/etm.2017.4997
  • Pages:3699-3707
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Age‑related macular degeneration (AMD) is associated with the dysfunction and death of the retinal pigment epithelium (RPE). Recently, there has been increasing interest in stem cell‑derived RPE cells for cell replacement therapies, such as those for AMD. The present study investigated whether RPE‑conditioned medium (RPECM) could promote the differentiation of human adipose tissue‑derived mesenchymal stromal cells (hADSCs) into RPE cells, and enhance the proliferation and migration of these cells. Reverse‑transcription quantitative polymerase chain reaction analysis demonstrated that RPECM induced hADSCs to differentiate into cells expressing RPE markers, including retinoid isomerohydrolase (RPE65), cytokeratin (CK8) and Bestrophin, which were identified to be significantly upregulated by ~10‑fold, 3.5‑fold and 2.4‑fold, respectively, compared with the control group [hADSCs cultured in ADSC‑conditioned medium (ADSCCM)]. The immunocytochemistry and western blot analysis results demonstrated that the protein levels of RPE65, CK8 and Bestrophin were significantly increased in RPECM‑treated hADSCs. In addition, Cell Counting Kit‑8 analysis demonstrated that RPECM promoted the proliferation of induced cells. RPECM also increased the expression level of the cell proliferative marker Ki‑67. Furthermore, to evaluate the migration potential, cell migration assays were performed. These assays demonstrated that following RPECM treatment hADSCs migrated more quickly compared with the control group. The results of the present study suggest that RPECM induces hADSCs to differentiate into RPE cells with higher proliferative and migratory potentials, which may aid in applications for hADSCs in RPE regenerative therapy.

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October 2017
Volume 14 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

2016 Impact Factor: 1.261
Ranked #50/128 Medicine Research and Experimental
(total number of cites)

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APA
Zhang, Y., Zhang, D., Wei, W., Shen, B., Wang, Y., Zhang, Y. ... Gu, P. (2017). Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration. Experimental and Therapeutic Medicine, 14, 3699-3707. https://doi.org/10.3892/etm.2017.4997
MLA
Zhang, Y., Zhang, D., Wei, W., Shen, B., Wang, Y., Zhang, Y., Zhang, Y., Ji, J., Sun, H., Luo, M., Gu, P."Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration". Experimental and Therapeutic Medicine 14.4 (2017): 3699-3707.
Chicago
Zhang, Y., Zhang, D., Wei, W., Shen, B., Wang, Y., Zhang, Y., Zhang, Y., Ji, J., Sun, H., Luo, M., Gu, P."Effects of RPE‑conditioned medium on the differentiation of hADSCs into RPE cells, and their proliferation and migration". Experimental and Therapeutic Medicine 14, no. 4 (2017): 3699-3707. https://doi.org/10.3892/etm.2017.4997