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Article Open Access

Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes

  • Authors:
    • Deshan Hu
    • Xiuli Shan
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, The Fifth People's Hospital of Jinan, Jinan, Shandong 250022, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5411-5416
    |
    Published online on: September 26, 2017
       https://doi.org/10.3892/etm.2017.5202
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Abstract

The objective of this study was to analyze the effects of type-I collagen hydrogel of different concentrations on the growth and differentiation of rabbit chondrocytes. Articular cartilage from New Zealand white rabbits was harvested and cultured. Second-generation chondrocytes were collected for in vitro culture with 10, 7, and 5 mg/ml type-I collagen hydrogel, respectively (denoted as groups A, B, and C). After in vitro culture for 1 day, chondrocytes were stained with fluorescein diacetate (FDA)/propidium iodide (PI), and cell viability was observed by laser confocal microscopy. After in vitro culture for 14 days, the histological patterns were observed by H&E and toluidine blue staining. The expression of chondrocyte-related genes were measured by real-time quantitative RT-PCR. After in vitro culture for 1 day, FDA/PI staining showed that the cell density of group A was significantly higher than that of group B and C. After in vitro culture for 14 days, H&E staining showed that chondrocytes showed obvious aggregation in group A, partial proliferation and aggregation in group B, and uniform distribution in group C. Toluidine blue staining showed that chondrocytes in group A had aggregation areas and some were stained purple-red, fewer chondrocytes were aggregated with different staining around them in group B, and the aggregation of chondrocytes was not obvious. However, the distribution of chondrocytes was uniform with different staining in group C. After in vitro culture for 2 weeks, the levels of polymerized proteoglycan and type-II collagen mRNA were not significantly different between the three groups (P>0.05). The levels of type-I collagen, type-X collagen, and Sox9 mRNA in group A were significantly higher than those in group B and C (P<0.05). In conclusion, high concentration type-I collagen hydrogel can promote chondrocyte fibrosis and upregulation of type-I collagen, type-X collagen, and Sox9 mRNA.
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View References

1 

Mahapatra C, Jin GZ and Kim HW: Alginate-hyaluronic acid-collagen composite hydrogel favorable for the culture of chondrocytes and their phenotype maintenance. Tissue Eng Regen Med. 13:538–546. 2016. View Article : Google Scholar

2 

Foldager CB, Pedersen M, Ringgaard S, Bünger C and Lind M: Chondrocyte gene expression is affected by very small iron oxide particles-labeling in long-term in vitro MRI tracking. J Magn Reson Imaging. 33:724–730. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Ge D, Zhang QS, Zabaleta J, Zhang Q, Liu S, Reiser B, Bunnell BA, Braun SE, O'Brien MJ, Savoie FH, et al: Doublecortin may play a role in defining chondrocyte phenotype. Int J Mol Sci. 15:6941–6960. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Tsai WB, Chen CH, Chen JF and Chang KY: The effects of types of degradable polymers on porcine chondrocyte adhesion, proliferation and gene expression. J Mater Sci Mater Med. 17:337–343. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Xin W, Heilig J, Paulsson M, Zaucke F and Collagen II: Regulates integrin expression profile and chondrocyte differentiation. Connect Tissue Res. 56:307–314. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Gurusinghe S, Young P, Michelsen J and Strappe P: Suppression of dedifferentiation and hypertrophy in canine chondrocytes through lentiviral vector expression of Sox9 and induced pluripotency stem cell factors. Biotechnol Lett. 37:1495–1504. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Dou Y, Li N, Zheng Y and Ge Z: Effects of fluctuant magnesium concentration on phenotype of the primary chondrocytes. J Biomed Mater Res A. 102:4455–4463. 2014.PubMed/NCBI

8 

Yu L, Ferlin KM, Nguyen BN and Fisher JP: Tubular perfusion system for chondrocyte culture and szp expression. J Biomed Mater Res A. 103:1864–1874. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Ko AR, Huh YH, Lee HC, Song WK, Lee YS and Chun JS: Identification and characterization of arginase II as a chondrocyte phenotype-specific gene. IUBMB Life. 58:597–605. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Duval E, Leclercq S, Elissalde JM, Demoor M, Galéra P and Boumédiene K: Hypoxia-inducible factor 1alpha inhibits the fibroblast-like markers type I and type III collagen during hypoxia-induced chondrocyte redifferentiation: Hypoxia not only induces type II collagen and aggrecan, but it also inhibits type I and type III collagen in the hypoxia-inducible factor 1alpha-dependent redifferentiation of chondrocytes. Arthritis Rheum. 60:3038–3048. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Johnson JS, Morscher MA, Jones KC, Moen SM, Klonk CJ, Jacquet R and Landis WJ: Gene expression differences between ruptured anterior cruciate ligaments in young male and female subjects. J Bone Joint Surg Am. 97:71–79. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Kontturi LS, Järvinen E, Muhonen V, Collin EC, Pandit AS, Kiviranta I, Yliperttula M and Urtti A: An injectable, in situ forming type II collagen/hyaluronic acid hydrogel vehicle for chondrocyte delivery in cartilage tissue engineering. Drug Deliv Transl Res. 4:149–158. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Heo J, Koh RH, Shim W, Kim HD, Yim HG and Hwang NS: Riboflavin-induced photo-crosslinking of collagen hydrogel and its application in meniscus tissue engineering. Drug Deliv Transl Res. 6:148–158. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Park H, Guo X, Temenoff JS, Tabata Y, Caplan AI, Kasper FK and Mikos AG: Effect of swelling ratio of injectable hydrogel composites on chondrogenic differentiation of encapsulated rabbit marrow mesenchymal stem cells in vitro. Biomacromolecules. 10:541–546. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Lien SM, Ko LY and Huang TJ: Effect of pore size on ECM secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering. Acta Biomater. 5:670–679. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Galván JA, García-Martínez J, Vázquez-Villa F, García-Ocaña M, García-Pravia C, Menéndez-Rodríguez P, González-del Rey C, Barneo-Serra L and de los Toyos JR: Validation of COL11A1/procollagen 11A1 expression in TGF-β1-activated immortalised human mesenchymal cells and in stromal cells of human colon adenocarcinoma. BMC Cancer. 14:8672014. View Article : Google Scholar : PubMed/NCBI

17 

Faikrua A, Wittaya-areekul S, Oonkhanond B and Viyoch J: A thermosensitive chitosan/corn starch/β-glycerol phosphate hydrogel containing TGF-β1 promotes differentiation of MSCs into chondrocyte-like cells. Tissue Eng Regen Med. 11:355–361. 2014. View Article : Google Scholar

18 

Jin R, Teixeira LS Moreira, Krouwels A, Dijkstra PJ, van Blitterswijk CA, Karperien M and Feijen J: Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. Acta Biomater. 6:1968–1977. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Schindler OS: Current concepts of articular cartilage repair. Acta Orthop Belg. 77:709–726. 2011.PubMed/NCBI

20 

Li P, Wei X, Guan Y, Chen Q, Zhao T, Sun C and Wei L: MicroRNA-1 regulates chondrocyte phenotype by repressing histone deacetylase 4 during growth plate development. FASEB J. 28:3930–3941. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Tew SR, Li Y, Pothacharoen P, Tweats LM, Hawkins RE and Hardingham TE: Retroviral transduction with SOX9 enhances re-expression of the chondrocyte phenotype in passaged osteoarthritic human articular chondrocytes. Osteoarthritis Cartilage. 13:80–89. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Darling EM and Athanasiou KA: Retaining zonal chondrocyte phenotype by means of novel growth environments. Tissue Eng. 11:395–403. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Zhang M and Wang J: Epigenetic regulation of gene expression in osteoarthritis. Genes Dis. 2:69–75. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Balakrishnan B and Banerjee R: Biopolymer-based hydrogels for cartilage tissue engineering. Chem Rev. 111:4453–4474. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hu D and Shan X: Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes. Exp Ther Med 14: 5411-5416, 2017.
APA
Hu, D., & Shan, X. (2017). Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes. Experimental and Therapeutic Medicine, 14, 5411-5416. https://doi.org/10.3892/etm.2017.5202
MLA
Hu, D., Shan, X."Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes". Experimental and Therapeutic Medicine 14.6 (2017): 5411-5416.
Chicago
Hu, D., Shan, X."Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5411-5416. https://doi.org/10.3892/etm.2017.5202
Copy and paste a formatted citation
x
Spandidos Publications style
Hu D and Shan X: Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes. Exp Ther Med 14: 5411-5416, 2017.
APA
Hu, D., & Shan, X. (2017). Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes. Experimental and Therapeutic Medicine, 14, 5411-5416. https://doi.org/10.3892/etm.2017.5202
MLA
Hu, D., Shan, X."Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes". Experimental and Therapeutic Medicine 14.6 (2017): 5411-5416.
Chicago
Hu, D., Shan, X."Effects of different concentrations of type-I collagen hydrogel on the growth and differentiation of chondrocytes". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5411-5416. https://doi.org/10.3892/etm.2017.5202
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