Quercetin‑loaded mixed micelles exhibit enhanced cytotoxic efficacy in non‑small cell lung cancer in vitro

Zhao,Ming‑Hong; Yuan,Lin; Meng,Ling‑Yun; Qiu,Jian‑Ling; Wang,Chun‑Bin

doi:10.3892/etm.2017.5230

Quercetin‑loaded mixed micelles exhibit enhanced cytotoxic efficacy in non‑small cell lung cancer in vitro

Authors:
- Ming‑Hong Zhao
- Lin Yuan
- Ling‑Yun Meng
- Jian‑Ling Qiu
- Chun‑Bin Wang
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Affiliations: Department of Oncology, Jianhu People's Hospital, Yancheng, Jiangsu 224700, P.R. China, Department of Oncology, Yancheng Third People's Hospital, Yancheng, Jiangsu 224001, P.R. China
Published online on: September 29, 2017 https://doi.org/10.3892/etm.2017.5230
Pages: 5503-5508

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Abstract

In the present study, quercetin (QUR)‑loaded mixed micelles (QUR‑M) were prepared with the aim of improving the physicochemical and anticancer efficacy of QUR in lung cancer cells. The mixed micelles comprised tocopheryl polyethylene glycol 1000 succinate (TPGS) and a 1,2‑distearoyl‑sn‑glycero‑3‑phosphatidylethanolamine derivative of polyethylene glycol. The nanosized QUR‑M exhibited a pH‑responsive and controlled release of QUR that is likely to be beneficial in cancer treatment. The results of an MTT assay clearly demonstrated that the anticancer effect of QUR‑M in A549 cancer cells was stronger compared with that of free QUR at 24 and 48 h time points. The half‑maximal inhibitory concentrations of QUR and QUR‑M were observed to be 12.45 and 6.42 µg/ml, respectively. When stained with Hoechst 33342 and observed using a confocal laser scanning microscope, A549 cells treated with QUR‑M exhibited severe chromatin condensation and apoptotic body formation of the nuclei. Overall, high intracellular uptake, sustained drug release and the presence of TPGS in the mixed micelles may result in an increased inhibitory effect against cell proliferation and improved therapeutic efficacy in lung cancers.

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