Niu‑Huang‑Shen suppresses hepatocellular carcinoma cell growth and metastasis by regulating Yap1 expression

Peng,Yanfang; Zhao,Yingqian; Ye,Taisheng; Zhang,Yabing; Wu,Zhaoyan; Xia,Yukun; Zhang,Yingwen

doi:10.3892/etm.2017.5247

December-2017 Volume 14 Issue 6

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December-2017 Volume 14 Issue 6

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Article Open Access

Niu‑Huang‑Shen suppresses hepatocellular carcinoma cell growth and metastasis by regulating Yap1 expression

Authors:
- Yanfang Peng
- Yingqian Zhao
- Taisheng Ye
- Yabing Zhang
- Zhaoyan Wu
- Yukun Xia
- Yingwen Zhang
View Affiliations / Copyright

Affiliations: Department of Traditional Chinese Medicine, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China

Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5459-5463
|
Published online on: October 2, 2017

https://doi.org/10.3892/etm.2017.5247
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Abstract

Hepatocellular carcinoma (HCC) is one of the most common cancers types. Niu‑Huang‑Shen (NHS), a Chinese medicine, has been reported to exert antipyretic, anti‑inflammatory and vasodilatation effects. However, whether NHS has inhibitory effects on HCC cell phenotypes has remained elusive. In the present study, Cell Counting Kit‑8, colony formation, fluorescence-activated cell sorting and Transwell assays were used to evaluate the effect of NHS on cell proliferation, migration and invasion. The results indicated that NHS suppressed cell proliferation and invasion, inhibited cell apoptosis, and induced cell cycle arrest. In addition, NHS significantly suppressed the mRNA and protein expression of Yes‑associated protein (YAP). It was concluded that NHS downregulated YAP expression and inhibited the Hippo signaling pathway as well as HCC cell growth and invasion. NHS may be a novel potential therapeutic for HCC patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Niu‑Huang‑Shen suppresses hepatocellular carcinoma cell growth and metastasis by regulating Yap1 expression

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