Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis

  • Authors:
    • Zhen‑Zhen Chen
    • Xin Gong
  • View Affiliations / Copyright

    Affiliations: School of Traditional Chinese Medicine, Beijing Key Lab of TCM Collateral Disease Theory Research, Capital Medical University, Beijing 100069, P.R. China, Department of Gynecology, Dong Fang Hospital of Beijing University of Chinese Medicine, Beijing 100078, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5743-5750
    |
    Published online on: October 11, 2017
       https://doi.org/10.3892/etm.2017.5280
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aims of the present study were to investigate the effects of a traditional Chinese medicine, Hua Yu Xiao Zheng (HYXZ) decoction, on surgically induced endometriosis in a rat model and to determine the possible underlying regulatory mechanisms. A total of 108 female Sprague‑Dawley rats were divided into the control group (n=12) and endometriosis group (EM group; n=96), in which endometriosis was surgically induced in model rats by autotransplantation of endometrial tissues and 72 rats survived. After 3 weeks, the EM model rats were randomly divided into four subgroups (n=18), including the untreated model group, and three groups administered 7, 14 or 21 g/kg HYXZ decoction. Following 28 days of treatment, the associated proteins and genes of ectopic endometrial tissues were analyzed using immunohistochemistry, western blotting and quantitative polymerase chain reaction to investigate the underlying mechanisms. Compared with the model group, the size of the endometriotic implants decreased significantly in the HYXZ‑treated groups. Furthermore, the expression levels of vascular endothelial growth factor (VEGF) and angiopoietin‑2 (Ang‑2) were significantly decreased in HYXZ‑treated groups compared with the model group. These results indicate that HYXZ affected the inhibition of angiogenesis and decreased the endometriotic implant volumes and histopathological scores. The effectiveness of HYXZ may be partially attributed to the decrease of VEGF and Ang‑2 expression levels in the ectopic endometrium.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Burney RO and Giudice LC: Pathogenesis and pathophysiology of endometriosis. Fertil Steril. 98:511–519. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Jacobson TZ, Duffy JM, Barlow DH, Koninckx PR and Garry R: WITHDRAWN: Laparoscopic surgery for pelvic pain associated with endometriosis. Cochrane Database Syst Rev: CD001300. 2014. View Article : Google Scholar

3 

Sampson JA: Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation. Am J Pathol. 3:93–110.43. 1927.PubMed/NCBI

4 

Groothuis PG, Nap AW, Winterhager E and Grümmer R: Vascular development in endometriosis. Angiogenesis. 8:147–156. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Mueller MD, Vigne JL, Minchenko A, Lebovic DI, Leitman DC and Taylor RN: Regulation of vascular endothelial growth factor (VEGF) gene transcription by estrogen receptors alpha and beta. Proc Natl Acad Sci USA. 97:pp. 10972–10977. 2000, View Article : Google Scholar : PubMed/NCBI

6 

Rocha AL, Reis FM and Taylor RN: Angiogenesis and endometriosis. Obstet Gynecol Int. 2013:8596192013.PubMed/NCBI

7 

Donnez J, Smoes P, Gillerot S, Casanas-Roux F and Nisolle M: Vascular endothelial growth factor (VEGF) in endometriosis. Hum Reprod. 13:1686–1690. 1998. View Article : Google Scholar : PubMed/NCBI

8 

Charnock-Jones DS, Sharkey AM, Rajput-Williams J, Burch D, Schofield JP, Fountain SA, Boocock CA and Smith SK: Identification and localization of alternately spliced mRNAs for vascular endothelial growth factor in human uterus and estrogen regulation in endometrial carcinoma cell lines. Biol Reprod. 48:1120–1128. 1993. View Article : Google Scholar : PubMed/NCBI

9 

Healy DL, Rogers PA, Hii L and Wingfield M: Angiongenesis: A new theory for endometriosis. Hum Reprod Update. 4:736–740. 1998. View Article : Google Scholar : PubMed/NCBI

10 

Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ and Holash J: Vascular-specific growth factors and blood vessel formation. Nature. 407:242–248. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Jingting C, Yangde Z, Yi Z, Mengxiong L, Rong Y, Yu Z, Guoqing P and Lixiu P: Expression of heparanase and angiopoietin-2 in patients with endometriosis. Eur J Obstet Gynecol Reprod Biol. 136:199–209. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Loukovaara S, Robciuc A, Holopainen JM, Lehti K, Pessi T, Liinamaa J, Kukkonen KT, Jauhiainen M, Koli K, Keski-Oja J and Immonen I: Ang-2 upregulation correlates with increased levels of MMP-9, VEGF, EPO and TGFβ1 in diabetic eyes undergoing vitrectomy. Acta Ophthalmol. 91:531–539. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Weigel MT, Krämer J, Schem C, Wenners A, Alkatout I, Jonat W, Maass N and Mundhenke C: Differential expression of MMP-2, MMP-9 and PCNA in endometriosis and endometrial carcinoma. Eur J Obstet Gynecol Reprod Biol. 74–78. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Hur SE, Lee JY, Moon HS and Chung HW: Angiopoietin-1, angiopoietin-2 and Tie-2 expression in eutopic endometrium in advanced endometriosis. Mol Hum Reprod. 12:421–426. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Wieser F, Cohen M, Gaeddert A, Yu J, Burks-Wicks C, Berga SL and Taylor RN: Evolution of medical treatment for endometriosis: Back to the roots? Hum Reprod Update. 13:487–499. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Yang M: Treatment of endometriosis by comprehensive TCM therapy: A clinical observation of 35 cases. New J Tradit Chin Med. 2004.PubMed/NCBI

17 

Tsai PJ, Lin YH, Chen JL, Yang SH, Chen YC and Chen HY: Identifying Chinese herbal medicine network for endometriosis: Implications from a population-based database in Taiwan. Evid Based Complement Alternat Med. 2017:75010152017. View Article : Google Scholar : PubMed/NCBI

18 

Gao H, Wang Z, Li Y and Qian Z: Overview of the quality standard research of traditional Chinese medicine. Frontiers of Medicine. 5:195–202. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Gong X, Lou J, Lu Q, Huang H and Jin Z: Bu Shen Huo Xue decoction restores endometrial leukemia-inhibitory factor but not Angiopoietin-2 expression, and improves uterine receptivity in the controlled ovarian stimulation rat model. Exp Ther Med. 9:751–757. 2015.PubMed/NCBI

20 

Zhang H, Li J, Sun W, Hu Y, Zhang G, Shen M and Shi X: Hyaluronic acid-modified magnetic iron oxide nanoparticles for MR imaging of surgically induced endometriosis model in rats. PLoS One. 9:e947182014. View Article : Google Scholar : PubMed/NCBI

21 

Jiao L, Qi X, Lu G, Zhang Q, Zhang C and Gao J: Effect of traditional Chinese medicine (Xiaochaihu Tang) on the expression of MMP-2 and MMP-9 in rats with endometriosis. Exp Ther Med. 6:1385–1389. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Reagan-Shaw S, Nihal M and Ahmad N: Dose translation from animal to human studies revisited. FASEB J. 22:659–661. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Yu J, Zhao L, Zhang D, Zhai D, Shen W, Bai L, Liu Y, Cai Z, Li J and Yu C: The effects and possible mechanisms of puerarin to treat endometriosis model rats. Evid Based Complement Alternat Med. 2015:2691382015. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Jiang HQ, Li YL and Zou J: Effect of recombinant human endostatin on endometriosis in mice. Chin Med J (Engl). 120:1241–1246. 2007.PubMed/NCBI

26 

Lucidi RS, Witz CA, Chrisco M, Binkley PA, Shain SA and Schenken RS: A novel in vitro model of the early endometriotic lesion demonstrates that attachment of endometrial cells to mesothelial cells is dependent on the source of endometrial cells. Fertil Steril. 84:16–21. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Anderson FW and Johnson CT: Complementary and alternative medicine in obstetrics. Int J Gynaecol Obstet. 91:116–124. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Tindle HA, Davis RB, Phillips RS and Eisenberg DM: Trends in use of complementary and alternative medicine by US adults: 1997–2002. Altern Ther Health Med. 11:42–49. 2005.PubMed/NCBI

29 

Flower A, Liu JP, Lewith G, Little P and Li Q: Chinese herbal medicine for endometriosis. Cochrane Database Syst Rev: CD006568. 2012. View Article : Google Scholar

30 

Giudice LC: Clinical practice. Endometriosis. N Engl J Med. 362:2389–2398. 2010. View Article : Google Scholar : PubMed/NCBI

31 

McLaren J: Vascular endothelial growth factor and endometriotic angiogenesis. Hum Reprod Update. 6:45–55. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Folkman J and Shing Y: Angiogenesis. J Biol Chem. 267:10931–10934. 1992.PubMed/NCBI

33 

Shifren JL, Tseng JF, Zaloudek CJ, Ryan IP, Meng YG, Ferrara N, Jaffe RB and Taylor RN: Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium: Implications for angiogenesis during the menstrual cycle and in the pathogenesis of endometriosis. J Clin Endocrinol Metab. 81:3112–3118. 1996. View Article : Google Scholar : PubMed/NCBI

34 

Niedźwiecki S, Stepień T, Kopeć K, Kuzdak K, Komorowski J, Krupiński R and Stepień H: Angiopoietin 1 (Ang-1), angiopoietin 2 (Ang-2) and Tie-2 (a receptor tyrosine kinase) concentrations in peripheral blood of patients with thyroid cancers. Cytokine. 36:291–295. 2006. View Article : Google Scholar : PubMed/NCBI

35 

Hur SE, Lee JY, Moon HS and Chung HW: Angiopoietin-1, angiopoietin-2 and Tie-2 expression in eutopic endometrium in advanced endometriosis. Mol Hum Reprod. 12:421–426. 2006. View Article : Google Scholar : PubMed/NCBI

36 

He LL, Zhang WJ, Su H and Xu DG: Synergism between Ang-2 and VEGF and its application of anti-angiogenesis in tumor therapy - review. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 15:445–448. 2007.(In Chinese). PubMed/NCBI

37 

Bohn KA, Adkins CE, Nounou MI and Lockman PR: Inhibition of VEGF and angiopoietin-2 to reduce brain metastases of breast cancer burden. Front Pharmacol. 8:1932017. View Article : Google Scholar : PubMed/NCBI

38 

Yeniel AÖ, Erbas O, Ergenoglu AM, Aktug H, Taskiran D, Yildirim N and Ulukus M: Effect of oxytocin treatment on explant size, plasma and peritoneal levels of MCP-1, VEGF, TNF-α and histopathological parameters in a rat endometriosis model. Eur J Obstet Gynecol Reprod Biol. 175:134–139. 2014. View Article : Google Scholar : PubMed/NCBI

39 

Liu S, Xin X, Hua T, Shi R, Chi S, Jin Z and Wang H: Efficacy of Anti-VEGF/VEGFR agents on animal models of endometriosis: A systematic review and meta-analysis. PLoS One. 11:e01666582016. View Article : Google Scholar : PubMed/NCBI

40 

Hu C, Wang Z, Pang Z, Lu W, Cai X, Yang J, Wang D and Cao P: Guizhi fuling capsule, an ancient Chinese formula, attenuates endometriosis in rats via induction of apoptosis. Climacteric. 17:410–416. 2014. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen ZZ and Gong X: Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis. Exp Ther Med 14: 5743-5750, 2017.
APA
Chen, Z., & Gong, X. (2017). Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis. Experimental and Therapeutic Medicine, 14, 5743-5750. https://doi.org/10.3892/etm.2017.5280
MLA
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14.6 (2017): 5743-5750.
Chicago
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5743-5750. https://doi.org/10.3892/etm.2017.5280
Copy and paste a formatted citation
x
Spandidos Publications style
Chen ZZ and Gong X: Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis. Exp Ther Med 14: 5743-5750, 2017.
APA
Chen, Z., & Gong, X. (2017). Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis. Experimental and Therapeutic Medicine, 14, 5743-5750. https://doi.org/10.3892/etm.2017.5280
MLA
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14.6 (2017): 5743-5750.
Chicago
Chen, Z., Gong, X."Effect of Hua Yu Xiao Zheng decoction on the expression levels of vascular endothelial growth factor and angiopoietin‑2 in rats with endometriosis". Experimental and Therapeutic Medicine 14, no. 6 (2017): 5743-5750. https://doi.org/10.3892/etm.2017.5280
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team