Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital

Moustos,Emmanuel; Staphylaki,Dimitra; Christidou,Athanasia; Spandidos,Demetrios  A.; Neonakis,Ioannis  K.

doi:10.3892/etm.2017.5291

December-2017 Volume 14 Issue 6

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December-2017 Volume 14 Issue 6

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Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital

Authors:
- Emmanuel Moustos
- Dimitra Staphylaki
- Athanasia Christidou
- Demetrios A. Spandidos
- Ioannis K. Neonakis
View Affiliations / Copyright

Affiliations: Department of Clinical Microbiology and Microbial Pathogenesis, University Hospital of Heraklion, 71201 Heraklion, Crete, Greece

Copyright: © Moustos et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 6074-6076
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Published online on: October 11, 2017

https://doi.org/10.3892/etm.2017.5291
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Abstract

The knowledge of the expected time-to-positivity (TTP) of blood cultures by major pathogens is essential both clinically and economically. To this end, we conducted the present two‑year study in our Institution, aiming to assess the TTP of all the major microorganisms including Enterobacteriaceae, Pseudomonas aeruginosa, Acinetoacter baumannii, Enterococcii spp, Staphylococcus aureus and yeasts, to determine whether a 3‑day interval is sufficient for their detection. The TTP for each case of strain isolation per patient was determined as the TTP of the first bottle among a set of bottles collected within the same period of time to be flagged as positive per patient. Based on our results, almost all major Gram‑negative (99.30%), Gram‑positive microbia (99.01%) and yeasts (98.85%) were detected within the first 5‑days of incubation, leading to the solid conclusion that a 5‑day period of incubation is adequate to detect almost all the major routine pathogens. By contrast, when a 3‑day period was examined acceptable results were only found for Gram‑negative (98.33%) and Gram‑positive (98.51%) microbia. A significant proportion of yeasts (8.05%) could not be detected within this time frame. Therefore, regarding the yeasts, a 3-day incubation period cannot be considered as adequate and is not advocated.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Major pathogen microorganisms except yeasts can be detected from blood cultures within the first three days of incubation: A two‑year study from a University Hospital

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