Formononetin ameliorates mast cell‑mediated allergic inflammation via inhibition of histamine release and production of pro‑inflammatory cytokines

Xu,Ning; An,Jun

doi:10.3892/etm.2017.5293

Formononetin ameliorates mast cell‑mediated allergic inflammation via inhibition of histamine release and production of pro‑inflammatory cytokines

Authors:
- Ning Xu
- Jun An
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Affiliations: Department of Critical Care Medicine, Yulin Second Hospital, Yulin, Shaanxi 719000, P.R. China, Department of Respiration, 3201 Hospital, Hanzhong, Shaanxi 723000, P.R. China
Published online on: October 12, 2017 https://doi.org/10.3892/etm.2017.5293
Pages: 6201-6206

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Abstract

Various allergic diseases cause allergic inflammation, which is mediated by mast cells. The current study investigated the anti‑allergic inflammatory effects of formononetin and its mechanism of action in vitro using mast cells. Levels of histamine and pro‑inflammatory cytokines, including tumor necrosis factor‑α (TNF‑α), interleukin (IL)‑1β and IL‑6, were measured to assess the effects of formononetin on allergic inflammation. The activation of intracellular calcium and nuclear factor (NF)‑κB, as well as the activity of caspase‑1, were assessed to determine the mechanism of action. It was determined that difference concentrations of formononetin (0.1, 1 and 10 µM) suppressed histamine release and secretion of TNF‑α, IL‑1β and IL‑6. Further investigations indicated that the effects of formononetin were associated with a reduction of intracellular calcium, suppression of NF‑κB activation and upstream IκKα phosphorylation and inhibition of caspase‑1 activity. Therefore, the results of the current study demonstrated that formononetin ameliorated mast cell‑mediated allergic inflammation.

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1	Beghdadi W, Madjene LC, Benhamou M, Charles N, Gautier G, Launay P and Blank U: Mast cells as cellular sensors in inflammation and immunity. Front Immunol. 2:372011. View Article : Google Scholar : PubMed/NCBI
2	Galli SJ and Tsai M: IgE and mast cells in allergic disease. Nat Med. 18:693–704. 2012. View Article : Google Scholar : PubMed/NCBI
3	Kim HH, Bae Y and Kim SH: Galangin attenuates mast cell-mediated allergic inflammation. Food Chem Toxicol. 57:209–216. 2013. View Article : Google Scholar : PubMed/NCBI
4	Galli SJ, Tsai M and Piliponsky AM: The development of allergic inflammation. Nature. 454:445–454. 2008. View Article : Google Scholar : PubMed/NCBI
5	Bansal G, Xie Z, Rao S, Nocka KH and Druey KM: Suppression of immunoglobulin E-mediated allergic responses by regulator of G protein signaling 13. Nat Immunol. 9:73–80. 2008. View Article : Google Scholar : PubMed/NCBI
6	Je IG, Kim DS, Kim SW, Lee S, Lee HS, Park EK, Khang D and Kim SH: Tyrosol suppresses allergic inflammation by inhibiting the activation of phosphoinositide 3-kinase in mast cells. PLoS One. 10:e01298292015. View Article : Google Scholar : PubMed/NCBI
7	Kalesnikoff J and Galli SJ: New developments in mast cell biology. Nat Immunol. 9:1215–1223. 2008. View Article : Google Scholar : PubMed/NCBI
8	Hayden MS and Ghosh S: NF-κB in immunobiology. Cell Res. 21:223–244. 2011. View Article : Google Scholar : PubMed/NCBI
9	Lamkanfi M, Vande Walle L and Kanneganti TD: Deregulated inflammasome signaling in disease. Immunol Rev. 243:163–173. 2011. View Article : Google Scholar : PubMed/NCBI
10	Miao EA, Rajan JV and Aderem A: Caspase-1-induced pyroptotic cell death. Immunol Rev. 243:206–214. 2011. View Article : Google Scholar : PubMed/NCBI
11	Baba Y, Nishida K, Fujii Y, Hirano T, Hikida M and Kurosaki T: Essential function for the calcium sensor STIM1 in mast cell activation and anaphylactic responses. Nat Immunol. 9:81–88. 2008. View Article : Google Scholar : PubMed/NCBI
12	Li J, Jiang Z, Li X, Hou Y, Liu F, Li N, Liu X and Yang L: Natural therapeutic agents for neurodegenerative diseases from a traditional herbal medicine Pongamia pinnata (L.) Pierre. Bioorg Med Chem Lett. 25:53–58. 2015. View Article : Google Scholar : PubMed/NCBI
13	Li W, Sun YN, Yan XT, Yang SY, Kim S, Lee YM, Koh YS and Kim YH: Flavonoids from Astragalus membranaceus and their inhibitory effects on LPS-stimulated pro-inflammatory cytokine production in bone marrow-derived dendritic cells. Arch Pharm Res. 37:186–192. 2014. View Article : Google Scholar : PubMed/NCBI
14	Ghribi L, Waffo-Téguo P, Cluzet S, Marchal A, Marques J, Mérillon JM and Ben Jannet H: Isolation and structure elucidation of bioactive compounds from the roots of the Tunisian Ononis angustissima L. Bioorg Med Chem Lett. 25:3825–3830. 2015. View Article : Google Scholar : PubMed/NCBI
15	Tava A, Pecio Ł, Stochmal A and Pecetti L: Clovamide and flavonoids from leaves of Trifolium pratense and T. pratense subsp. nivale grown in Italy. Nat Prod Commun. 10:933–936. 2015.PubMed/NCBI
16	Sun T, Cao L, Ping NN, Wu Y, Liu DZ and Cao YX: Formononetin upregulates nitric oxide synthase in arterial endothelium through estrogen receptors and MAPK pathways. J Pharm Pharmacol. 68:342–351. 2016. View Article : Google Scholar : PubMed/NCBI
17	Wu XY, Xu H, Wu ZF, Chen C, Liu JY, Wu GN, Yao XQ, Liu FK, Li G and Shen L: Formononetin, a novel FGFR2 inhibitor, potently inhibits angiogenesis and tumor growth in preclinical models. Oncotarget. 6:44563–44578. 2015. View Article : Google Scholar : PubMed/NCBI
18	Hu W and Xiao Z: Formononetin induces apoptosis of human osteosarcoma cell line U2OS by regulating the expression of Bcl-2, Bax and MiR-375 in vitro and in vivo. Cell Physiol Biochem. 37:933–939. 2015. View Article : Google Scholar : PubMed/NCBI
19	Je IG, Kim HH, Park PH, Kwon TK, Seo SY, Shin TY and Kim SH: SG-HQ2 inhibits mast cell-mediated allergic inflammation through suppression of histamine release and pro-inflammatory cytokines. Exp Biol Med (Maywood). 240:631–638. 2015. View Article : Google Scholar : PubMed/NCBI
20	Bae Y, Lee S and Kim SH: Chrysin suppresses mast cell-mediated allergic inflammation: Involvement of calcium, caspase-1 and nuclear factor-κB. Toxicol Appl Pharmacol. 254:56–64. 2011. View Article : Google Scholar : PubMed/NCBI
21	Kim HH, Park SB, Lee S, Kwon TK, Shin TY, Park PH, Lee SH and Kim SH: Inhibitory effect of putranjivain A on allergic inflammation through suppression of mast cell activation. Toxicol Appl Pharmacol. 274:455–461. 2014. View Article : Google Scholar : PubMed/NCBI
22	Tanaka S, Mikura S, Hashimoto E, Sugimoto Y and Ichikawa A: Ca²⁺ influx-mediated histamine synthesis and IL-6 release in mast cells activated by monomeric IgE. Eur J Immunol. 35:460–468. 2005. View Article : Google Scholar : PubMed/NCBI
23	Lee NY, Chung KS, Jin JS, Bang KS, Eom YJ, Hong CH, Nugroho A, Park HJ and An HJ: Effect of chicoric acid on mast cell-mediated allergic inflammation in vitro and in vivo. J Nat Prod. 78:2956–2962. 2015. View Article : Google Scholar : PubMed/NCBI
24	Shen D, Xie X, Zhu Z, Yu X, Liu H, Wang H, Fan H, Wang D, Jiang G and Hong M: Screening active components from Yu-ping-feng-san for regulating initiative key factors in allergic sensitization. PLoS One. 9:e1072792014. View Article : Google Scholar : PubMed/NCBI
25	Besnard AG, Togbe D, Couillin I, Tan Z, Zheng SG, Erard F, Le Bert M, Quesniaux V and Ryffel B: Inflammasome-IL-1-Th17 response in allergic lung inflammation. J Mol Cell Biol. 4:3–10. 2012. View Article : Google Scholar : PubMed/NCBI