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Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis

  • Authors:
    • Li Guo
    • Yanli Liang
  • View Affiliations / Copyright

    Affiliations: Department of Liver Disease, Xuzhou Infectious Disease Hospital, Xuzhou, Jiangsu 221000, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6007-6011
    |
    Published online on: October 12, 2017
       https://doi.org/10.3892/etm.2017.5299
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Abstract

The present study was conducted to investigate the expression of apoptosis-related factors in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B and their correlation to clinical prognosis. Sixty-two patients that were diagnosed with chronic hepatitis B were admitted to Xuzhou Hospital from March 2015 to February 2016 and were enrolled as the observation group, while 60 healthy subjects who were examined in the health examination center were selected as the control group. The PBMC of patients were collected, and mRNA expression levels of the apoptotic molecules (FAS, CASP3, CASP8 and CASP9) were measured using real-time fluorescence quantitative PCR. The protein expression levels of apoptosis-related genes in the plasma were detected using enzyme-linked immunosorbent assay (ELISA) and the serum HBV-DNA was quantitatively measured using real-time fluorescence quantitative PCR. The mRNA and protein expression levels of FAS, CASP3, CASP8 and CASP9 in the observation group were significantly higher than those in the control group (p<0.05). The positive rate and log value of the copy amount of HBV DNA in the observation group were significantly higher than those in the control group (p<0.05). The Pearson correlation coefficient analysis showed that FAS, CASP3, CASP8 and CASP9 were positively correlated with HBV DNA (p<0.05). The mRNA expression levels of FAS, CASP3, CASP8 and CASP9 in patients with negative HBV-DNA were significantly lower than those with positive HBV-DNA (p<0.05). Apoptosis of PBMCs play an important role in the occurrence and development of chronic hepatitis B, and is closely correlated to the level of serum virus replication and prognosis.
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1 

Zeisel MB, Lucifora J, Mason WS, Sureau C, Beck J, Levrero M, Kann M, Knolle PA, Benkirane M, Durantel D, et al: Towards an HBV cure: State-of-the-art and unresolved questions - report of the ANRS workshop on HBV cure. Gut. 64:1314–1326. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Papastergiou V, Lombardi R, Macdonald D and Tsochatzis EA: Global epidemiology of hepatitis B virus (HBV) infection. Curr Hepat Rep. 14:171–178. 2015. View Article : Google Scholar

3 

Roberts H, Kruszon-Moran D, Ly KN, Hughes E, Iqbal K, Jiles RB and Holmberg SD: Prevalence of chronic hepatitis B virus (HBV) infection in U.S. households: National Health and Nutrition Examination Survey (NHANES), 1988–2012. Hepatology. 63:388–397. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Luo G, Feng X, Huang Y, Yi T, Wang D, Guo X, Yan H and Zhang G: Effects of antiviral therapy on the cellular immune response in patients with chronic hepatitis B. Mol Med Rep. 11:1284–1291. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Lamb C and Arbuthnot P: Activating the innate immune response to counter chronic hepatitis B virus infection. Expert Opin Biol Ther. 16:1517–1527. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Filippelli M, Garozzo MT, Capizzi A, Spina M, Manti S, Tardino L, Salpietro C and Leonardi S: Immune response to hepatitis B virus vaccine in celiac subjects at diagnosis. World J Hepatol. 8:1105–1109. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Himaja N and Shama SN: Herbal wealth for hepatotoxicity: A review. Asian J Pharm Clin Res. 8:3–9. 2015.

8 

Gonzalez SA and Keeffe EB: Chronic hepatitis B and C: Update on therapy. Future Virol. 4:437–452. 2015. View Article : Google Scholar

9 

World Health Organization (WHO), . WHO Guidelines Approved by the Guidelines Review Committee: WHO Guidelines for the Prevention, Care and Treatment of Persons with Chronic Hepatitis B Infection. World Health Organization; Geneva: 2015

10 

The Korean Association for the Study of the Liver (KASL): KASL Clinical Practice Guidelines: Management of chronic hepatitis B. Clin Mol Hepatol. 18:109–162. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Lv J, Lin S, Peng P, Cai C, Deng J, Wang M, Li X, Lin R, Lin Y, Fang A, et al: Arenobufagin activates p53 to trigger esophageal squamous cell carcinoma cell apoptosis in vitro and in vivo. Onco Targets Ther. 10:1261–1267. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Hong CA and Nam YS: Functional nanostructures for effective delivery of small interfering RNA therapeutics. Theranostics. 4:1211–1232. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Zhao BB, Zheng SJ, Gong LL, Wang Y, Chen CF, Jin WJ, Zhang D, Yuan XH, Guo J, Duan ZP, et al: T lymphocytes from chronic HCV-infected patients are primed for activation-induced apoptosis and express unique pro-apoptotic gene signature. PLoS One. 8:e770082013. View Article : Google Scholar : PubMed/NCBI

14 

Somal A, Aggarwal A and Upadhyay RC: Effect of thermal stress on expression profile of apoptosis related genes in peripheral blood mononuclear cells of transition Sahiwal cow. Iran J Vet Res. 16:137–143. 2015.PubMed/NCBI

15 

McIlwain DR, Berger T and Mak TW: Caspase functions in cell death and disease. Cold Spring Harb Perspect Biol. 7:a0086562015. View Article : Google Scholar

16 

Karev VE: Fas, FasL, and bcl-2 expression on hepatic intralobar lymphocytes in different variants of the natural course of chronic HBV and HCV infection and in its outcomes. Arkh Patol. 76:16–21. 2014.(In Russian). PubMed/NCBI

17 

Martins D'Oliveira F, Gomes BC, Rodrigues AS and Rueff J: Genetic susceptibility in acute pancreatitis: Genotyping of GSTM1, GSTT1, GSTP1, CASP7, CASP8, CASP9, CASP10, LTA, TNFRSF1B, and TP53 gene variants. Pancreas. 46:71–76. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Meng P, Yoshida H, Tanji K, Matsumiya T, Xing F, Hayakari R, Wang L, Tsuruga K, Tanaka H, Mimura J, et al: Carnosic acid attenuates apoptosis induced by amyloid-β 1–42 or 1–43 in SH-SY5Y human neuroblastoma cells. Neurosci Res. 94:1–9. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Guo X, Dong Z, Yamada S, Li Y, Guo Y, Shen S, Liang J, Tanimoto A and Guo W: Association of Casp3 microRNA target site (1049216) SNP with the risk and progress of cervical squamous cell carcinoma. Int J Gynecol Cancer. 27:206–213. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Peng CY, Hsieh TC, Hsieh TY, Tseng KC, Lin CL, Su TH, Tseng TC, Lin HH, Wang CC and Kao JH: HBV-DNA level at 6 months of entecavir treatment predicts HBeAg loss in HBeAg-positive chronic hepatitis B patients. J Formos Med Assoc. 114:308–313. 2015. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Guo L and Liang Y: Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis. Exp Ther Med 14: 6007-6011, 2017.
APA
Guo, L., & Liang, Y. (2017). Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis. Experimental and Therapeutic Medicine, 14, 6007-6011. https://doi.org/10.3892/etm.2017.5299
MLA
Guo, L., Liang, Y."Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis". Experimental and Therapeutic Medicine 14.6 (2017): 6007-6011.
Chicago
Guo, L., Liang, Y."Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6007-6011. https://doi.org/10.3892/etm.2017.5299
Copy and paste a formatted citation
x
Spandidos Publications style
Guo L and Liang Y: Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis. Exp Ther Med 14: 6007-6011, 2017.
APA
Guo, L., & Liang, Y. (2017). Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis. Experimental and Therapeutic Medicine, 14, 6007-6011. https://doi.org/10.3892/etm.2017.5299
MLA
Guo, L., Liang, Y."Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis". Experimental and Therapeutic Medicine 14.6 (2017): 6007-6011.
Chicago
Guo, L., Liang, Y."Expression of PBMC apoptosis-related factors in patients with chronic hepatitis B and their relationships with clinical prognosis". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6007-6011. https://doi.org/10.3892/etm.2017.5299
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