Isoforskolin downregulates proinflammatory responses induced by Borrelia burgdorferi basic membrane protein A

Zhao,Hua; Liu,Aihua; Shen,Longqiang; Xu,Cuiping; Zhu,Ziwei; Yang,Jiaru; Han,Xinling; Bao,Fukai; Yang,Weimin

doi:10.3892/etm.2017.5300

December-2017 Volume 14 Issue 6

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December-2017 Volume 14 Issue 6

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Article Open Access

Isoforskolin downregulates proinflammatory responses induced by Borrelia burgdorferi basic membrane protein A

Authors:
- Hua Zhao
- Aihua Liu
- Longqiang Shen
- Cuiping Xu
- Ziwei Zhu
- Jiaru Yang
- Xinling Han
- Fukai Bao
- Weimin Yang
View Affiliations / Copyright

Affiliations: Department of Microbiology and Immunology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China, Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan 650500, P.R. China, School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan 650500, P.R. China

Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5974-5980
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Published online on: October 13, 2017

https://doi.org/10.3892/etm.2017.5300
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Abstract

The plant Coleus forskohlii is distributed primarily in India, Thailand, China, Egypt and Brazil and has a history of use in the treatment of multiple diseases. Isoforskolin (ISOF) is the principle active component of C. forskohlii native to China and has previously been studied for its biological effects. The aim of the present study was to evaluate the effect of ISOF on the proinflammatory responses induced by recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). In in vitro experiments, the proinflammatory effects of rBmpA and the anti‑inflammatory function of ISOF were evaluated in murine macrophages, human macrophages and dendritic cells by detecting the transcription and expression of tumor necrosis factor (TNF)‑α and interleukin (IL)‑6. In in vivo experiments, mean arthritis index and X‑ray and histopathological examinations were used to verify the role of ISOF in experimental Lyme arthritis in mice. The results indicated that rBmpA, which induced the transcription and expression of TNF‑α and IL‑6, activated proinflammatory responses in murine macrophages, human macrophages and dendritic cells. In turn, ISOF downregulated the transcription and expression of TNF‑α and IL‑6 induced by rBmpA. Additionally, the in vivo experiments demonstrated that ISOF could also inhibit the symptoms of experimental Lyme arthritis. These results suggest that ISOF may have a potential application as an anti‑inflammatory agent for the treatment of Lyme arthritis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Isoforskolin downregulates proinflammatory responses induced by Borrelia burgdorferi basic membrane protein A

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