Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway

  • Authors:
    • Li Feng
    • Yehong Li
    • Wenchao Zeng
    • Bo Xia
    • Dongsheng Zhou
    • Jing Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250014, P.R. China, Department of Obstetrics and Gynecology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China
  • Pages: 6087-6092
    |
    Published online on: October 17, 2017
       https://doi.org/10.3892/etm.2017.5320
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the effects of basic fibroblast growth factor (bFGF) and fibronectin (FN) on adhesion of osteoblasts seeded into bio‑derived bone scaffolds. Rat calvarial osteoblasts were separated and their osteogenic phenotypes were determined by staining for alkaline phosphatase as well as alizarin red staining. The bio‑derived bone scaffolds were prepared from the metaphysis of porcine femur and their physicochemical properties were assessed by scanning electron microscopy (SEM) and X‑ray diffraction analysis. An MTT assay was used to detect the effects of bFGF and FN on osteoblast adhesion or proliferation on cell/scaffold constructs through blocking the extracellular matrix FN‑integrin pathway by the Gly‑Arg‑Gly‑Asp‑Ser (GRGDS) peptide. Western blot analysis was used to measure the β1 integrin levels. Based on the adhesion of osteoblasts stimulated by various concentrations of bFGF (0.1, 1, 10 and 100 ng/ml) on bio‑derived bone scaffolds modified by various concentrations of FN (0.1, 1, 10 and 100 µg/ml), the cell/scaffold constructs were divided into four groups: i) Control, non‑stimulated and non‑modified group; ii) 10 µg/ml FN‑modified group; iii) 100 ng/ml bFGF‑stimulated group; iv) 10 µg/ml FN + 100 ng/ml bFGF group. Cell proliferation curves acquired by MTT assay and micrographs obtained by SEM showed that the combination of bFGF and FN significantly improved cell adhesion, particularly in the 10 µg/ml FN + 100 ng/ml bFGF group vs. the other groups, and the effect on cell adhesion was inhibited by 1 mmol/l GRGDS peptide through the FN‑integrin pathway. Western blot results showed that the combination of bFGF and FN significantly enhanced β1 integrin expression levels. These results suggested that osteoblasts stimulated by 100 ng/ml bFGF and bio‑derived bone materials modified by 10 µg/ml FN should be combined to be applied in the bone tissue engineering.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Liu F, Zhang X, Yu X, Xu Y, Feng T and Ren D: In vitro study in stimulating the secretion of angiogenic growth factors of strontium-doped calcium polyphosphate for bone tissue engineering. J Mater Sci Mater Med. 22:683–692. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Zhang ZY, Teoh SH, Chong WS, Foo TT, Chng YC, Choolani M and Chan J: A biaxial rotating bioreactor for the culture of fetal mesenchymal stem cells for bone tissue engineering. Biomaterials. 30:2694–2704. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Xie H, Yang F, Deng L, Luo J, Qin T, Li X, Zhou GQ and Yang Z: The performance of a bone-derived scaffold material in the repair of critical bone defects in a rhesus monkey model. Biomaterials. 28:3314–3324. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Lee S, Lee DS, Choi I, Le Pham BH and Jang JH: Design of an osteoinductive extracellular fibronectin matrix protein for bone tissue engineering. Int J Mol Sci. 16:7672–7681. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Pegueroles M, Aparicio C, Bosio M, Engel E, Gil FJ, Planell JA and Altankov G: Spatial organization of osteoblast fibronectin matrix on titanium surfaces: Effects of roughness, chemical heterogeneity and surface energy. Acta Biomater. 6:291–301. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Calderwood DA, Shattil SJ and Ginsberg MH: Integrins and actin filaments: Reciprocal regulation of cell adhesion and signaling. J Biol Chem. 275:22607–22610. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Brunner M, Millon-Frémillon A, Chevalier G, Nakchbandi IA, Mosher D, Block MR, Albigès-Rizo C and Bouvard D: Osteoblast mineralization requires beta1 integrin/ICAP-1-dependent fibronectin deposition. J Cell Biol. 194:307–322. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Bentmann A, Kawelke N, Moss D, Zentgraf H, Bala Y, Berger I, Gasser JA and Nakchbandi IA: Circulating fibronectin affects bone matrix, whereas osteoblast fibronectin modulates osteoblast function. J Bone Miner Res. 25:706–715. 2010.PubMed/NCBI

9 

He L, Lin Y, Hu X, Zhang Y and Wu H: A comparative study of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) on the effect of proliferation and differentiation of rat osteoblasts in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 108:707–713. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Houseman BT and Mrksich M: The microenvironment of immobilized Arg-Gly-Asp peptides is an important determinant of cell adhesion. Biomaterials. 22:943–955. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Shin H, Zygourakis K, Farach-Carson MC, Yaszemski MJ and Mikos AG: Attachment, proliferation, and migration of marrow stromal osteoblasts cultured on biomimetic hydrogels modified with an osteopontin-derived peptide. Biomaterials. 25:895–906. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Yuan Q, Kubo T, Doi K, Morita K, Takeshita R, Katoh S, Shiba T, Gong P and Akagawa Y: Effect of combined application of bFGF and inorganic polyphosphate on bioactivities of osteoblasts and initial bone regeneration. Acta Biomater. 5:1716–1724. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Li P, Bai Y, Yin G, Pu X, Huang Z, Liao X, Chen X and Yao Y: Synergistic and sequential effects of BMP-2, bFGF and VEGF on osteogenic differentiation of rat osteoblasts. J Bone Miner Metab. 32:627–635. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Tang DZ, Hou W, Zhou Q, Zhang M, Holz J, Sheu TJ, Li TF, Cheng SD, Shi Q, Harris SE, et al: Osthole stimulates osteoblast differentiation and bone formation by activation of beta-catenin-BMP signaling. J Bone Miner Res. 25:1234–1245. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Trivedi R, Kumar S, Kumar A, Siddiqui JA, Swarnkar G, Gupta V, Kendurker A, Dwivedi AK, Romero JR and Chattopadhyay N: Kaempferol has osteogenic effect in ovariectomized adult sprague-dawley rats. Mol Cell Endocrinol. 289:85–93. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Huo K, Zhang X, Wang H, Zhao L, Liu X and Chu PK: Osteogenic activity and antibacterial effects on titanium surfaces modified with Zn-incorporated nanotube arrays. Biomaterials. 34:3467–3478. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Yao D, Xie XH, Wang XL, Wan C, Lee YW, Chen SH, Pei DQ, Wang YX, Li G and Qin L: Icaritin, an exogenous phytomolecule, enhances osteogenesis but not angiogenesis-an in vitro efficacy study. PLoS One. 7:e412642012. View Article : Google Scholar : PubMed/NCBI

18 

Zhang ZY, Teoh SH, Chong MS, Schantz JT, Fisk NM, Choolani MA and Chan J: Superior osteogenic capacity for bone tissue engineering of fetal compared with perinatal and adult mesenchymal stem cells. Stem cells. 27:126–137. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Lin YC, Brayfield CA, Gerlach JC, Rubin JP and Marra KG: Peptide modification of polyethersulfone surfaces to improve adipose-derived stem cell adhesion. Acta Biomater. 5:1416–1424. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Mariscal-Muñoz E, Costa CA, Tavares HS, Bianchi J, Hebling J, Machado JP, Lerner UH and Souza PP: Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb: YAG laser irradiation. Clin Oral Investig. 20:503–511. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Wang YW, Wu Q and Chen GQ: Attachment, proliferation and differentiation of osteoblasts on random biopolyester poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffolds. Biomaterials. 25:669–675. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Li T, Li H, Li T, Fan J, Zhao RC and Weng X: MicroRNA expression profile of dexamethasone-induced human bone marrow-derived mesenchymal stem cells during osteogenic differentiation. J Cell Biochem. 115:1683–1691. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Hendesi H, Barbe MF, Safadi FF, Monroy MA and Popoff SN: Integrin mediated adhesion of osteoblasts to connective tissue growth factor (CTGF/CCN2) induces cytoskeleton reorganization and cell differentiation. PLoS One. 10:e01153252015. View Article : Google Scholar : PubMed/NCBI

24 

Ishida K and Haudenschild DR: Interactions between FGF21 and BMP-2 in osteogenesis. Biochem Biophys Res Commun. 432:677–682. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Yoshimoto H, Shin YM, Terai H and Vacanti JP: A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials. 24:2077–2082. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Wang H, Li Y, Zuo Y, Li J, Ma S and Cheng L: Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials. 28:3338–3348. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Ribeiro N, Sousa SR and Monteiro FJ: Influence of crystallite size of nanophased hydroxyapatite on fibronectin and osteonectin adsorption and on MC3T3-E1 osteoblast adhesion and morphology. J Colloid Interface Sci. 351:398–406. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Moursi AM, Globus RK and Damsky CH: Interactions between integrin receptors and fibronectin are required for calvarial osteoblast differentiation in vitro. J Cell Sci. 110:2187–2196. 1997.PubMed/NCBI

29 

Lim JY, Taylor AF, Li Z, Vogler EA and Donahue HJ: Integrin expression and osteopontin regulation in human fetal osteoblastic cells mediated by substratum surface characteristics. Tissue Eng. 11:19–29. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Tang CH, Yang RS, Huang TH, Liu SH and Fu WM: Enhancement of fibronectin fibrillogenesis and bone formation by basic fibroblast growth factor via protein kinase c-dependent pathway in rat osteoblasts. Mol Pharmacol. 66:440–449. 2004.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng L, Li Y, Zeng W, Xia B, Zhou D and Zhou J: Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway. Exp Ther Med 14: 6087-6092, 2017.
APA
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., & Zhou, J. (2017). Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway. Experimental and Therapeutic Medicine, 14, 6087-6092. https://doi.org/10.3892/etm.2017.5320
MLA
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., Zhou, J."Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway". Experimental and Therapeutic Medicine 14.6 (2017): 6087-6092.
Chicago
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., Zhou, J."Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6087-6092. https://doi.org/10.3892/etm.2017.5320
Copy and paste a formatted citation
x
Spandidos Publications style
Feng L, Li Y, Zeng W, Xia B, Zhou D and Zhou J: Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway. Exp Ther Med 14: 6087-6092, 2017.
APA
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., & Zhou, J. (2017). Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway. Experimental and Therapeutic Medicine, 14, 6087-6092. https://doi.org/10.3892/etm.2017.5320
MLA
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., Zhou, J."Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway". Experimental and Therapeutic Medicine 14.6 (2017): 6087-6092.
Chicago
Feng, L., Li, Y., Zeng, W., Xia, B., Zhou, D., Zhou, J."Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix‑integrin pathway". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6087-6092. https://doi.org/10.3892/etm.2017.5320
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team