Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December-2017 Volume 14 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9

  • Authors:
    • Qingde Wa
    • Peiheng He
    • Shuai Huang
    • Jianwei Zuo
    • Xing Li
    • Jinsong Zhu
    • Song Hong
    • Guoqing Lv
    • Dongfeng Cai
    • Dongliang Xu
    • Xuenong Zou
    • Yi Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The First Affiliated Hospital, Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China, Department of Orthopedic Surgery, The First Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Sports Medicine, Shenzhen Hospital of Peking University, Shenzhen, Guangdong 518036, P.R. China
  • Pages: 6131-6137
    |
    Published online on: October 18, 2017
       https://doi.org/10.3892/etm.2017.5344
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to investigate the mechanisms underlying microRNA (miRNA)‑mediated regulation of chondrogenic differentiation. Mouse embryo‑derived stem cells C3H10T1/2 were cultured and chondrogenic differentiation was induced using transforming growth factor‑β3 (TGF‑β3). In addition, miRNA expression profiles were detected via miRNA array analysis, and quantitative polymerase chain reaction was performed to verify the differentially expressed miRNAs. Furthermore, bioinformatics software was used to predict the putative targets and the prediction was validated by dual‑luciferase reporter assays and western blot analysis. In addition, cell proliferation and glycosaminoglycans were measured by a direct cell count method and alcian blue staining, respectively. Compared with the control group, 86 miRNAs were identified as differentially expressed in TGF‑β3‑induced cells and the expression levels of 28 miRNAs were increased while the remaining 58 miRNAs exhibited a decline in expression. Amongst the differentially expressed miRNAs, miR‑30b expression was observed to have significantly decreased during chondrogenic differentiation. SOX9 is a target gene of miR‑30b, and miR‑30b inhibits SOX9 expression during chondrogenic differentiation. Furthermore, the alcian blue staining results demonstrated that miR‑30b inhibited early chondrogenic differentiation. However, the data of the present study indicated that miR‑30b had no influence on C3H10T1/2 cell line proliferation. In conclusion, miR‑30b is a key negative regulator of TGF‑β3‑induced C3H10T1/2 cell chondrogenic differentiation, which functions by directly targeting SOX9.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

He L and Hannon GJ: MicroRNAs: Small RNAs with a big role in gene regulation. Nat Rev Genet. 5:522–531. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Kanellopoulou C, Muljo SA, Kung AL, Ganesan S, Drapkin R, Jenuwein T, Livingston DM and Rajewsky K: Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev. 19:489–501. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Luzi E, Marini F, Sala SC, Tognarini I, Galli G and Brandi ML: Osteogenic differentiation of human adipose tissue-derived stem cells is modulated by the miR-26a targeting of the SMAD1 transcription factor. J Bone Miner Res. 23:287–295. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Ansorge WJ: Next-generation DNA sequencing techniques. N Biotechnol. 25:195–203. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Wang Z, Gerstein M and Snyder M: RNA-Seq: A revolutionary tool for transcriptomics. Nat Rev Genet. 10:57–63. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Martin JA and Wang Z: Next-generation transcriptome assembly. Nat Rev Genet. 12:671–682. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Studer D, Millan C, Öztürk E, Maniura-Weber K and Zenobi-Wong M: Molecular and biophysical mechanisms regulating hypertrophic differentiation in chondrocytes and mesenchymal stem cells. Eur Cell Mater. 24:118–135. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Hsiao SH, Lee KD, Hsu CC, Tseng MJ, Jin VX, Sun WS, Hung YC, Yeh KT, Yan PS, Lai YY, et al: DNA methylation of the Trip10 promoter accelerates mesenchymal stem cell lineage determination. Biochem Biophys Res Commun. 400:305–12. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Xian CJ and Foster BK: Repair of injured articular and growth plate cartilage using mesenchymal stem cells and chondrogenic gene therapy. Curr Stem Cell Res Ther. 1:213–29. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Ji YH, Ji JL, Sun FY, Zeng YY, He XH, Zhao JX, Yu Y, Yu SH and Wu W: Quantitative proteomics analysis of chondrogenic differentiation of C3H10T1/2 mesenchymal stem cells by iTRAQ labeling coupled with on-line two-dimensional LC/MS/MS. Mol Cell Proteomics. 9:550–564. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Ji C, Liu X, Xu L, Yu T, Dong C and Luo J: RUNX1 plays an important role in mediating BMP9-Induced osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12 and MEFs. Int J Mol Sci. 18:E13482017. View Article : Google Scholar : PubMed/NCBI

12 

Son HE, Kim TH and Jang WG: Curculactones A and B induced the differentiation of C3H10T1/2 and MC3T3-E1 cells to osteoblasts. Bioorg Med Chem Lett. 27:1301–1303. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Hashimoto Y, Kobayashi M, Matsuzaki E, Higashi K, Takahashi-Yanaga F, Takano A, Hirata M and Nishimura F: Sphingosine-1-phosphate-enhanced Wnt5a promotes osteogenic differentiation in C3H10T1/2cells. Cell Biol Int. 40:1129–1136. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Megosh HB, Cox DN, Campbell C and Lin H: The role of PIWI and the miRNA machinery in drosophila germline determination. Curr Biol. 16:1884–1894. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Park JK, Liu X, Strauss TJ, McKearin DM and Liu Q: The miRNA pathway intrinsically controls self-renewal of drosophila germline stem cells. Curr Biol. 17:533–538. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Förstemann K, Tomari Y, Du T, Vagin VV, Denli AM, Bratu DP, Klattenhoff C, Theurkauf WE and Zamore PD: Normal microRNA maturation and germ-line stem cell maintenance requires loquacious, a double-stranded RNA-binding domain protein. PLoS Biol. 3:e2362005. View Article : Google Scholar : PubMed/NCBI

17 

Wang Y, Medvid R, Melton C, Jaenisch R and Blelloch R: DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal. Nat Genet. 39:380–385. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Wa Q, Gao M, Dai X, Yu T, Zhou Z, Xu D and Zou X: Induction of chondrogenic differentiation of mouse embryonic mesenchymal stem cells through an in vitro pellet model. Cell Biol Int. 39:657–665. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Carmell MA, Girard A, van de Kant HJ, Bourc'his D, Bestor TH, de Rooij DG and Hannon GJ: MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Dev Cell. 12:503–514. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Chen CZ, Li L, Lodish HF and Bartel DP: MicroRNAs modulate hematopoietic lineage differentiation. Science. 303:83–86. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Hornstein E, Mansfield JH, Yekta S, Hu JK, Harfe BD, McManus MT, Baskerville S, Bartel DP and Tabin CJ: The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development. Nature. 438:671–674. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Pfeffer S and Voinnet O: Viruses, microRNAs and cancer. Oncogene. 25:6211–6219. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Taft RJ, Pang KC, Mercer TR, Dinger M and Mattick JS: Non-coding RNAs: Regulators of disease. J Pathol. 220:126–139. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Fukumoto T, Sperling JW, Sanyal A, Fitzsimmons JS, Reinholz GG, Conover CA and O'Driscoll SW: Combined effects of insulin-like growth factor-1 and transforming growth factor-beta1 on periosteal mesenchymal cells during chondrogenesis in vitro. Osteoarthritis Cartilage. 11:55–64. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Schaefer JF, Millham ML, de Crombrugghe B and Buckbinder L: FGF signaling antagonizes cytokine-mediated repression of Sox9 in SW1353 chondrosarcoma cells. Osteoarthritis Cartilage. 11:233–241. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Murakami S, Kan M, McKeehan WL and de Crombrugghe B: Up-regulation of the chondrogenic Sox9 gene by fibroblast growth factors is mediated by the mitogen-activated protein kinase pathway. Proc Natl Acad Sci USA. 97:pp. 1113–1118. 2000, View Article : Google Scholar : PubMed/NCBI

28 

Woods A and Beier F: RhoA/ROCK signaling regulates chondrogenesis in a context-dependent manner. J Biol Chem. 281:13134–13140. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Tamamura Y, Katsube K, Mera H, Itokazu M and Wakitani S: Irx3 and Bmp2 regulate mouse mesenchymal cell chondrogenic differentiation in both a Sox9-dependent and -independent manner. J Cell Physiol. 232:3317–3336. 2017. View Article : Google Scholar : PubMed/NCBI

30 

Bridgewater LC, Lefebvre V and de Crombrugghe B: Chondrocyte-specific enhancer elements in the Col11a2 gene resemble the Col2a1 tissue-specific enhancer. J Biol Chem. 273:14998–15006. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Sekiya I, Tsuji K, Koopman P, Watanabe H, Yamada Y, Shinomiya K, Nifuji A and Noda M: SOX9 enhances aggrecan gene promoter/enhancer activity and is up-regulated by retinoic acid in a cartilage-derived cell line, TC6. J Biol Chem. 275:10738–10744. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Xie WF, Zhang X, Sakano S, Lefebvre V and Sandell LJ: Trans-activation of the mouse cartilage-derived retinoic acid-sensitive protein gene by Sox9. J Bone Miner Res. 14:757–763. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Sakou T: Bone morphogenetic proteins: From basic studies to clinical approaches. Bone. 22:591–603. 1998. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wa Q, He P, Huang S, Zuo J, Li X, Zhu J, Hong S, Lv G, Cai D, Xu D, Xu D, et al: miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9. Exp Ther Med 14: 6131-6137, 2017.
APA
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J. ... Liu, Y. (2017). miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9. Experimental and Therapeutic Medicine, 14, 6131-6137. https://doi.org/10.3892/etm.2017.5344
MLA
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J., Hong, S., Lv, G., Cai, D., Xu, D., Zou, X., Liu, Y."miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9". Experimental and Therapeutic Medicine 14.6 (2017): 6131-6137.
Chicago
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J., Hong, S., Lv, G., Cai, D., Xu, D., Zou, X., Liu, Y."miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6131-6137. https://doi.org/10.3892/etm.2017.5344
Copy and paste a formatted citation
x
Spandidos Publications style
Wa Q, He P, Huang S, Zuo J, Li X, Zhu J, Hong S, Lv G, Cai D, Xu D, Xu D, et al: miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9. Exp Ther Med 14: 6131-6137, 2017.
APA
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J. ... Liu, Y. (2017). miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9. Experimental and Therapeutic Medicine, 14, 6131-6137. https://doi.org/10.3892/etm.2017.5344
MLA
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J., Hong, S., Lv, G., Cai, D., Xu, D., Zou, X., Liu, Y."miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9". Experimental and Therapeutic Medicine 14.6 (2017): 6131-6137.
Chicago
Wa, Q., He, P., Huang, S., Zuo, J., Li, X., Zhu, J., Hong, S., Lv, G., Cai, D., Xu, D., Zou, X., Liu, Y."miR-30b regulates chondrogenic differentiation of mouse embryo‑derived stem cells by targeting SOX9". Experimental and Therapeutic Medicine 14, no. 6 (2017): 6131-6137. https://doi.org/10.3892/etm.2017.5344
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team