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Article

Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster

  • Authors:
    • Meng Wang
    • Yangyang Ou Yang
    • Qingtao Jin
    • Linlin Shang
    • Jian Zhang
  • View Affiliations / Copyright

    Affiliations: Medical Department, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Gastroenterology, Jining No. 1 People's Hospital, Jining, Shandong 272011, P.R. China, Department of Gastroenterology, Jiaxiang People's Hospital, Jining, Shandong 272400, P.R. China, Department of Pharmacy, Jining No. 2 People's Hospital, Jining, Shandong 272011, P.R. China
  • Pages: 440-446
    |
    Published online on: October 23, 2017
       https://doi.org/10.3892/etm.2017.5358
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Abstract

MicroRNAs (miRNAs/miRs) are a class of small, non‑coding RNA molecules that serve a key function in carcinogenesis and tumor progression. Recent evidence indicates that miRNAs may act as powerful regulators of migration and invasion. The present study aimed to investigate the effect of miR‑25 on the invasion and metastasis of KYSE‑150 and EC109 esophageal squamous cell carcinoma (ESCC) cells, and predict the mechanism of this effect by bioinformatically analyzing the miR‑106b‑25 cluster. In order to alter the expression of miR‑25 in the two cell lines, a miR‑25 inhibitor or mimic were transfected into the cells, which were then studied via Transwell migration and invasion assays. Subsequently, the target genes of the miR‑106b‑25 cluster were predicted using miRanda, PicTar, TargetScan and miRTarbase, and the functions of the target genes were predicted via Gene Ontology term and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Then, a protein‑protein interaction (PPI) network was produced using the Search Tool for the Retrieval of Interacting Genes. The results revealed that overexpressing miR‑25 led to significantly increased cell migration and invasion in KYSE150 and EC109 cells. Suppressing miR‑25 resulted in significantly decreased cell migration and invasion in KYSE150 cells, while the result was not significant in EC109 cells. Target genes of the miR‑106b‑25 cluster were significantly enriched in the biological process regulation of cellular metabolic process and several cancer‑associated pathways, such as those for glioma and melanoma. The PPI network revealed that PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may serve core roles within the network and associate with one another during the pathogenesis of ESCC. These results indicate that a high expression of miR‑25 promotes the invasion and metastasis of ESCC cells, while the influence of low expression of miR‑25 differs with cells with different degrees of differentiation. Invasion and metastasis are not effected in cells with poor differentiation, while they were decreased in well differentiated cells. Furthermore, PTEN, TP53, MDM2, E2F1, PRMT5, MCM2, RB1, CDKN1A, SHAD7 and EZH2 may be targeted by the miR‑106b‑25 cluster, and act together to regulate the development of ESCC.
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1 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2008. View Article : Google Scholar

2 

Ning Z, Zhu H, Li F, Liu Q, Liu G, Tan T, Zhang B, Chen S, Li G, Huang D, et al: Tumor suppression by miR-31 in esophageal carcinoma is p21-dependent. Genes Cancer. 5:436–444. 2014.PubMed/NCBI

3 

Guohong Z, Min S, Duenmei W, Songnian H, Min L, Jinsong L, Hongbin L, Feng Z, Dongping T, Heling Y, et al: Genetic heterogeneity of oesophageal cancer in high-incidence areas of southern and northern China. PLoS One. 5:e96682010. View Article : Google Scholar : PubMed/NCBI

4 

Zhao Z, Liu J, Wang C, Wang Y, Jiang Y and Guo M: MicroRNA-25 regulates small cell lung cancer cell development and cell cycle through cyclin E2. Int J Clin Exp Pathol. 7:7726–7734. 2014.PubMed/NCBI

5 

Li HL, Xie SP, Yang YL, Cheng YX, Zhang Y, Wang J, Wang Y, Liu DL, Chen ZF, Zhou YN and Wu HY: Clinical significance of upregulation of mir-196a-5p in gastric cancer and enriched kegg pathway analysis of target genes. Asian Pac J Cancer Prev. 16:1781–1787. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Lee RC, Feinbaum RL and Ambros V: The C. Elegans Heterochronic Gene lin-4 Encodes Small RNAs with Antisense Complementarity to &II-14. Cell. 75:843–854. 1993. View Article : Google Scholar : PubMed/NCBI

7 

Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR and Ruvkun G: The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 403:901–906. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Lee NK, Lee JH, Park CH, Yu D, Lee YC, Cheong JH, Noh SH and Lee SK: Long non-coding HOTAIR promotes carcinogenesis and invasion of gastric adenocarcinoma. Biochem Biophys Res Commun. 451:171–178. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Carninci P, Kasukawa T, Katayama S, Gough J, Frith MC, Maeda N, Oyama R, Ravasi T, Lenhard B, Wells C, et al: The transcriptional landscape of the mammalian genome. Science. 309:1559–1563. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Lai EC, Tomancak P, Williams RW and Rubin GM: Computational identification of Drosophila microRNA genes. Genome Biology. 4:R422003. View Article : Google Scholar : PubMed/NCBI

11 

Axtell MJ, Westholm JO and Lai EC: Vive la différence: Biogenesis and evolution of microRNAs in plants and animals. Genome Biol. 12:2212011. View Article : Google Scholar : PubMed/NCBI

12 

Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T and Margalit H: Clustering and conservation patterns of human microRNAs. Nucleic Acids Res. 33:2697–2706. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Liu R, Liao J, Yang M, Sheng J, Yang H, Wang Y, Pan E, Guo W, Pu Y, Kim SJ and Yin L: The Cluster of miR-143 and miR-145 affects the risk for esophageal squamous cell carcinoma through co-regulating fascin homolog. PLoS One. 7:e339872012. View Article : Google Scholar : PubMed/NCBI

14 

Khuu C, Utheim TP and Sehic A: The Three Paralogous MicroRNA Clusters in Development and Disease, miR-17-92, miR-106a-363, andmiR-106b-25. Scientifica (Cairo). 2016:13796432016.PubMed/NCBI

15 

Smith AL, Iwanaga R, Drasin DJ, Micalizzi DS, Vartuli RL, Tan AC and Ford HL: The miR-106b-25 cluster targets Smad7, activates TGF-β signaling and induces EMT and tumor initiating cell characteristics downstream of Six1 in human breast cancer. Oncogene. 31:5162–5171. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Feber A, Xi L, Luketich JD, Pennathur A, Landreneau RJ, Wu M, Swanson SJ, Godfrey TE and Litle VR: MicroRNA Expression Profiles of Esophageal Cancer. J Thorac Cardiovasc Surg. 135:255–260. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Antonov AV, Dietmann S and Mewes HW: KEGG spider: interpretation of genomics data in the context of the global gene metabolic network. Genome Biol. 9:R1792008. View Article : Google Scholar : PubMed/NCBI

19 

Kimura S, Naganuma S, Susuki D, Hirono Y, Yamaguchi A, Fujieda S, Sano K and Itoh H: Expression of microRNA in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC. Oncol Rep. 23:1625–1633. 2010.PubMed/NCBI

20 

Matsumura Y, Hiraoka K, Ishikawa K, Shoji Y, Noji T, Hontani K, Itoh T, Nakamura T, Tsuchikawa T, Shichinohe T and Hirano S: CD40 expression in human esophageal squamous cell carcinoma is associated with tumor progression and lymph node metastasis. Anticancer Res. 36:4467–4475. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Li Y, Tan W, Neo TW, Aung MO, Wasser S, Lim SG and Tan TM: Role of the miR-106b-25 microRNA cluster in hepatocellular carcinoma. Cancer Sci. 100:1234–1242. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Kan T, Sato F, Ito T, Matsumura N, David S, Cheng Y, Agarwal R, Paun BC, Jin Z, Olaru AV, et al: The miR-106b-25 polycistron, activated by genomic amplification, functions as an oncogene by suppressing p21 and Bim. Gastroenterology. 136:1689–1700. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Petrocca F, Visone R, Onelli MR, Shah MH, Nicoloso MS, de Martino I, Iliopoulos D, Pilozzi E, Liu CG, Negrini M, et al: E2F1-regulated microRNAs impair TGFbeta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell. 13:272–286. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Spender LC and Inman GJ: TGF-beta induces growth arrest in Burkitt lymphoma cells via transcriptional repression of E2F-1. J Biol Chem. 284:1435–1442. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Chen HZ, Tsai SY and Leone G: Emerging roles of E2Fs in cancer: An exit from cell cycle control. Nat Rev Cancer. 9:785–797. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Saha MN, Qiu L and Chang H: Targeting p53 by small molecules in hematological malignancies. J Hematol Oncol. 6:232013. View Article : Google Scholar : PubMed/NCBI

27 

Xu X, Chen Z, Zhao X, Wang J, Ding D, Wang Z, Tan F, Tan X, Zhou F, Sun J, et al: MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma. Biochem Biophys Res Commun. 421:640–645. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Fu X, Tian J, Zhang L, Chen Y and Hao Q: Involvement of microRNA-93, a new regulator of PTEN/Akt signaling pathway, in regulation of chemotherapeutic drug cisplatin chemosensitivity in ovarian cancer cells. FEBS Lett. 586:1279–1286. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Poliseno L, Salmena L, Riccardi L, Fornari A, Song MS, Hobbs RM, Sportoletti P, Varmeh S, Egia A, Fedele G, et al: Identification of the miR-106b~25 microRNA cluster as a proto-oncogenic PTEN-targeting intron that cooperates with its host gene MCM7 in transformation. Sci Signal. 3:ra292010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang M, Yang YO, Jin Q, Shang L and Zhang J: Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster. Exp Ther Med 15: 440-446, 2018.
APA
Wang, M., Yang, Y.O., Jin, Q., Shang, L., & Zhang, J. (2018). Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster. Experimental and Therapeutic Medicine, 15, 440-446. https://doi.org/10.3892/etm.2017.5358
MLA
Wang, M., Yang, Y. O., Jin, Q., Shang, L., Zhang, J."Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster". Experimental and Therapeutic Medicine 15.1 (2018): 440-446.
Chicago
Wang, M., Yang, Y. O., Jin, Q., Shang, L., Zhang, J."Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster". Experimental and Therapeutic Medicine 15, no. 1 (2018): 440-446. https://doi.org/10.3892/etm.2017.5358
Copy and paste a formatted citation
x
Spandidos Publications style
Wang M, Yang YO, Jin Q, Shang L and Zhang J: Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster. Exp Ther Med 15: 440-446, 2018.
APA
Wang, M., Yang, Y.O., Jin, Q., Shang, L., & Zhang, J. (2018). Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster. Experimental and Therapeutic Medicine, 15, 440-446. https://doi.org/10.3892/etm.2017.5358
MLA
Wang, M., Yang, Y. O., Jin, Q., Shang, L., Zhang, J."Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster". Experimental and Therapeutic Medicine 15.1 (2018): 440-446.
Chicago
Wang, M., Yang, Y. O., Jin, Q., Shang, L., Zhang, J."Function of miR‑25 in the invasion and metastasis of esophageal squamous carcinoma cells and bioinformatical analysis of the miR-106b-25 cluster". Experimental and Therapeutic Medicine 15, no. 1 (2018): 440-446. https://doi.org/10.3892/etm.2017.5358
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