The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes

Du,Zhaojin; Qiu,Zhilei; Wang,Zhankun; Wang,Xinsheng

doi:10.3892/etm.2017.5359

The inhibitory effects of soybean isoflavones on testicular cell apoptosis in mice with type 2 diabetes

Authors:
- Zhaojin Du
- Zhilei Qiu
- Zhankun Wang
- Xinsheng Wang
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Affiliations: Reproductive Medical Center, Qingdao Women and Children's Hospital, Qingdao University, Qingdao, Shandong 266034, P.R. China, Department of Urology, Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China, Department of Urology, The Eighth People's Hospital of Qingdao, Qingdao, Shandong 266121, P.R. China, Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
Published online on: October 24, 2017 https://doi.org/10.3892/etm.2017.5359
Pages: 305-309
Copyright: © Du et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the study was to investigate the inhibitory effects of soybean isoflavones (SI) on testicular cell apoptosis in mice with type-2 diabetes, as well as any possible mechanisms of action. Thirty male C57BL/6J mice were randomly divided into the control, diabetic (model), and treatment (SI) groups (n=10 each). After treatment for 20 weeks, testicular cell apoptosis was detected and evaluated using DAPI staining. The expression and distribution of caspase-3 protein in testicular tissues was detected via immunohistochemistry, while caspase-3 mRNA expression was detected using RT-PCR. Bax and Bcl-2 protein expression was detected by western blot analysis. At week 20, DAPI staining showed that SI treatment significantly decreased testicular tissue cell apoptosis in diabetic mice. Immunohistochemical staining revealed that caspase-3 expression in the SI group was significantly reduced relative to the model group. RT-PCR showed that SI treatment significantly decreased caspase-3 mRNA expression relative to the model group. Western blot analysis revealed that SI treatment significantly decreased Bax protein expression and increased Bcl-2 protein expression (P<0.01). SI exhibited an inhibitory effect on testicular tissue cell apoptosis in mice with type 2 diabetes, with this effect possibly mediated by a decreased expression of caspase-3 and Bax and increased Bcl-2 protein expression.

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