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Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2

  • Authors:
    • Lei Tan
    • Zhongyu Hou
    • Yanzhi Gao
  • View Affiliations / Copyright

    Affiliations: Traumatology Ward I, Zhangqiu Hospital of Chinese Medicine, Zhangqiu, Shandong 250200, P.R. China, Traumatology Ward II, Laiwu Iron and Steel Group Co. Ltd. Hospital, Laiwu, Shandong 271126, P.R. China, Department of Emergency, The Central Hospital of Shengli Oilfield, Dongying, Shandong 257034, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 288-294
    |
    Published online on: October 24, 2017
       https://doi.org/10.3892/etm.2017.5360
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Abstract

The objective of this study was to investigate the efficacy of combined treatment with vacuum sealing drainage (VSD) and recombinant human epidermal growth factor (rhEGF) for refractory wounds in the extremities, and its effect on serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and IL-2. Ninety-eight patients with refractory wounds in the extremities were recruited and randomly divided into the combined treatment group (underwent VSD and rhEGF treatment) and control group (underwent VSD only) with 49 cases each. Formation of granulation tissue on the wound surface was assessed and scored. The wound healing rate was calculated after 1 week of treatment, and the time of complete healing was recorded. Serum levels of IL-6, IL-2, and TNF-α were measured using enzyme-linked immunosorbent assay. After 1 week of treatment, granulation tissue formation on wound surfaces was significantly improved (p<0.05) compared with that before treatment in both groups. Moreover, granulation tissue formation on wound surfaces was superior in the combined treatment group than in the control group (p<0.05). The wound healing rate was 63.50±4.75% in the combined treatment group and 31.79±3.52% in the control group, and the difference was statistically significant (p<0.05). The time of complete healing was 15.11±2.24 days in the combined treatment group and 19.63±2.76 days in the control group, and the difference was statistically significant (p<0.05). The serum levels of IL-6, IL-2, and TNF-α, in the two groups were significantly lower than those before treatment (p<0.05). Moreover, the levels in the combined treatment group were significantly lower than those in the control group (p<0.05). In conclusion, combined treatment with VSD and rhEGF reduced inflammation and shortened the time of complete healing of refractory wounds in the extremities. Measurement of the levels of related inflammatory factors provided a reference for the prognosis of refractory wounds.
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Copy and paste a formatted citation
Spandidos Publications style
Tan L, Hou Z and Gao Y: Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2. Exp Ther Med 15: 288-294, 2018.
APA
Tan, L., Hou, Z., & Gao, Y. (2018). Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2. Experimental and Therapeutic Medicine, 15, 288-294. https://doi.org/10.3892/etm.2017.5360
MLA
Tan, L., Hou, Z., Gao, Y."Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2". Experimental and Therapeutic Medicine 15.1 (2018): 288-294.
Chicago
Tan, L., Hou, Z., Gao, Y."Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2". Experimental and Therapeutic Medicine 15, no. 1 (2018): 288-294. https://doi.org/10.3892/etm.2017.5360
Copy and paste a formatted citation
x
Spandidos Publications style
Tan L, Hou Z and Gao Y: Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2. Exp Ther Med 15: 288-294, 2018.
APA
Tan, L., Hou, Z., & Gao, Y. (2018). Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2. Experimental and Therapeutic Medicine, 15, 288-294. https://doi.org/10.3892/etm.2017.5360
MLA
Tan, L., Hou, Z., Gao, Y."Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2". Experimental and Therapeutic Medicine 15.1 (2018): 288-294.
Chicago
Tan, L., Hou, Z., Gao, Y."Efficacy of combined treatment with vacuum sealing drainage and recombinant human epidermal growth factor for refractory wounds in the extremities and its effect on serum levels of IL-6, TNF-α and IL-2". Experimental and Therapeutic Medicine 15, no. 1 (2018): 288-294. https://doi.org/10.3892/etm.2017.5360
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