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Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism

  • Authors:
    • Ying Qin
    • Shutong Zhuang
    • Jianfeng Wen
    • Kai Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518029, P.R. China
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 191-197
    |
    Published online on: October 24, 2017
       https://doi.org/10.3892/etm.2017.5370
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Abstract

Gastric cancer is the third leading cause of cancer‑associated mortality worldwide and is one of the most common malignancies in China. However, the molecular mechanisms underlying the tumorigenesis of gastric cancer remain largely unclear. Long non‑coding (Lnc)RNAs have been demonstrated to serve significant roles in the tumorigenesis of various types of cancer. The present study aimed to explore the role of the LncRNA mediator of DNA damage checkpoint protein 1‑antisense RNA (MDC1‑AS), the antisense transcript of MDC1, in human gastric cancer. The results revealed that the expression of MDC1‑AS in human gastric cancer was significantly suppressed in vivo and in vitro. In addition, overexpression of MDC1‑AS in the poorly differentiated gastric cancer cell line MKN28 significantly inhibited cell prolife­ration and metastasis, while the knockdown of MDC1‑AS in well‑differentiated MKN45 gastric cancer cells significantly increased proliferation and metastasis. The knockdown of MDC1 relieved the inhibitory effect of MDC1‑AS on MKN28 cell proliferation and metastasis, while the overexpression of MDC1 attenuated the stimulatory effect of MDC1‑AS knockdown in MKN45 cells. Thus, the present study demonstrated that MDC1‑AS had an inhibitory on gastric tumorigenesis through an MDC1‑dependent mechanism. This indicates that MDC1‑AS is a potential novel therapeutic target for the diagnosis and treatment of human gastric cancer in the clinic.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Cunningham D, Okines AF and Ashley S: Capecitabine and oxaliplatin for advanced esophagogastric cancer. N Engl J Med. 362:858–859. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Nagini S: Carcinoma of the stomach: A review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol. 4:156–169. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Kamangar F, Dores GM and Anderson WF: Patterns of cancer incidence, mortality, and prevalence across five continents: Defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 24:2137–2150. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Li R, Zhu H and Luo Y: Understanding the functions of long non-coding RNAs through their higher-order structures. Int J Mol Sci. 17:pii: E7022016. View Article : Google Scholar

9 

Qi P, Zhou XY and Du X: Circulating long non-coding RNAs in cancer: Current status and future perspectives. Mol Cancer. 15:392016. View Article : Google Scholar : PubMed/NCBI

10 

Harrow J, Frankish A, Gonzalez JM, Tapanari E, Diekhans M, Kokocinski F, Aken BL, Barrell D, Zadissa A, Searle S, et al: GENCODE: The reference human genome annotation for the ENCODE project. Genome Res. 22:1760–1774. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Fatica A and Bozzoni I: Long non-coding RNAs: New players in cell differentiation and development. Nat Rev Genet. 15:7–21. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Pauli A, Rinn JL and Schier AF: Non-coding RNAs as regulators of embryogenesis. Nat Rev Genet. 12:136–149. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Qi P, Zhou XY and Du X: Circulating long non-coding RNAs in cancer: Current status and future perspectives. Mol Cancer. 15:392016. View Article : Google Scholar : PubMed/NCBI

14 

Nie F, Yu X, Huang M, Wang Y, Xie M, Ma H, Wang Z, De W and Sun M: Long noncoding RNA ZFAS1 promotes gastric cancer cells proliferation by epigenetically repressing KLF2 and NKD2 expression. Oncotarget. 8:38227–38238. 2017.PubMed/NCBI

15 

Shao Y, Ye M, Li Q, Sun W, Ye G, Zhang X, Yang Y, Xiao B and Guo J: LncRNA-RMRP promotes carcinogenesis by acting as a miR-206 sponge and is used as a novel biomarker for gastric cancer. Oncotarget. 7:37812–37824. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Xue Y, Ma G, Zhang Z, Hua Q, Chu H, Tong N, Yuan L, Qin C, Yin C, Zhang Z and Wang M: A novel antisense long noncoding RNA regulates the expression of MDC1 in bladder cancer. Oncotarget. 6:484–493. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Yue H, Zhu J, Xie S, Li F and Xu Q: MDC1-AS, an antisense long noncoding RNA, regulates cell proliferation of glioma. Biomed Pharmacother. 81:203–209. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Chang AH and Parsonnet J: Role of bacteria in oncogenesis. Clin Microbiol Rev. 23:837–857. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Ruddon RW: Introduction to the molecular biology of cancer: Translation to the clinic. Prog Mol Biol Transl Sci. 95:1–8. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Montenegro RC, Clark PG, Howarth A, Wan X, Ceroni A, Siejka P, Nunez-Alonso GA, Monteiro O, Roger C, Gamble V, et al: BET inhibition as a new strategy for the treatment of gastric cancer. Oncotarget. 7:43997–44012. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Stewart GS, Wang B, Bignell CR, Taylor AM and Elledge SJ: MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature. 421:961–966. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Nakanishi M, Ozaki T, Yamamoto H, Hanamoto T, Kikuchi H, Furuya K, Asaka M, Delia D and Nakagawara A: NFBD1/MDC1 associates with p53 and regulates its function at the crossroad between cell survival and death in response to DNA damage. J Biol Chem. 282:22993–23004. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Derrien T, Johnson R, Bussotti G, Tanzer A, Djebali S, Tilgner H, Guernec G, Martin D, Merkel A, Knowles DG, et al: The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression. Genome Res. 22:1775–1789. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Morris KV and Vogt PK: Long antisense non-coding RNAs and their role in transcription and oncogenesis. Cell Cycle. 9:2544–2547. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Qin Y, Zhuang S, Wen J and Zheng K: Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism. Exp Ther Med 15: 191-197, 2018.
APA
Qin, Y., Zhuang, S., Wen, J., & Zheng, K. (2018). Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism. Experimental and Therapeutic Medicine, 15, 191-197. https://doi.org/10.3892/etm.2017.5370
MLA
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15.1 (2018): 191-197.
Chicago
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15, no. 1 (2018): 191-197. https://doi.org/10.3892/etm.2017.5370
Copy and paste a formatted citation
x
Spandidos Publications style
Qin Y, Zhuang S, Wen J and Zheng K: Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism. Exp Ther Med 15: 191-197, 2018.
APA
Qin, Y., Zhuang, S., Wen, J., & Zheng, K. (2018). Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism. Experimental and Therapeutic Medicine, 15, 191-197. https://doi.org/10.3892/etm.2017.5370
MLA
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15.1 (2018): 191-197.
Chicago
Qin, Y., Zhuang, S., Wen, J., Zheng, K."Long non‑coding RNA MDC1‑AS inhibits human gastric cancer cell proliferation and metastasis through an MDC1‑dependent mechanism". Experimental and Therapeutic Medicine 15, no. 1 (2018): 191-197. https://doi.org/10.3892/etm.2017.5370
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