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Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells

  • Authors:
    • Tiangang Ma
    • Danhua Qu
    • Bingdi Yan
    • Qinghua Zhang
    • Jin Ren
    • Yanbing Hu
  • View Affiliations / Copyright

    Affiliations: Department of Pneumology, The Second Hospital of Jilin University, Changchun, Jilin 130041, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 310-314
    |
    Published online on: October 27, 2017
       https://doi.org/10.3892/etm.2017.5380
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Abstract

A mutation in the IIb sodium phosphate transporter SLC34A2 gene has recently been described in pulmonary alveolar microlithiasis (PAM) patients. Experiments in this study were aimed at confirming the role of the gene product in PAM by comparing phosphorylated products in extracellular fluid of alveolar epithelial cells overexpressing the SLC34A2 gene or its mutated version. Eukaryotic expression vectors were constructed and transfected into A549 human alveolar epithelial cells. There were three groups of cells including those transfected with empty vector plasmid pcDNA3.1(+) (plasmid control group), those transfected with normal SLC34A2 gene expressed from pcDNA3.1 (normal control group), and those transfected with a version of the PAM SLC34A2 gene linked to the pcDNA3.1(+) (PAM group). Transfection efficiencies were detected by reverse transcription-polymerase chain reaction (RT-PCR). At 48 h after transfection, the concentration of inorganic phosphorus in the culture medium was detected using an automatic biochemical analyzer. Our results showed the concentration of inorganic phosphorus in the supernatant of the normal control group was significantly lower than that in the plasmid control and PAM groups (P<0.01), and the concentration in the PAM group was significantly lower than that in the plasmid control group (P<0.01). Based on our findings it is possible that the SLC34A2 gene mutation is the cause of the pathogenic changes observed in PAM patients, given that the function of the phosphate transporter seems to be affected and it is conceivable that it would lead to extracellular fluid alterations in vivo.
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1 

Samano MN, Waisberg DR, Canzian M, Campos SV, Pêgo-Fernandes PM and Jatene FB: Lung transplantation for pulmonary alveolar microlithiasis: A case report. Clinics (Sao Paulo). 65:233–236. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Jankovic S, Pavlov N, Ivkosic A, Erceg I, Glavina-Durdov M, Tocilj J, Dragisic-Ivulic S and Primorac D: Pulmonary alveolar microlithiasis in childhood: Clinical and radiological follow-up. Pediatr Pulmonol. 34:384–387. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Proesmans M, Boon M, Verbeken E, Ozcelik U, Kiper N, Van de Casseye W and De Boeck K: Pulmonary alveolar microlithiasis: A case report and review of the literature. Eur J Pediatr. 171:1069–1072. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Ishihara Y, Hagiwara K, Zen K, Huqun, Hosokawa Y and Natsuhara A: A case of pulmonary alveolar microlithiasis with an intragenetic deletion in SLC34A2 detected by a genome-wide SNP study. Thorax. 64:365–367. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Hagiwara K, Johkoh T and Tachibana T: Molecular basis of lung disease, insight from rare lung disordersPulmonary Alveolar Microlithiasis. Panos R, Trapnell B and McCormack F: Humana Press; Totowa, NJ: pp. 154–156. 2009

6 

Izumi S Huqun, Miyazawa H, Ishii K, Uchiyama B and Ishida T: The autozygous segments predicted by a genome-wide SNP typing revealed mutations in the type IIb sodium phosphate co-transporter (SLC34A2) causing pulmonary alveolar microlithiasis. Proc Am Thorac Soc. 3:pp. A1022006;

7 

Corut A, Senyigit A, Ugur SA, Altin S, Ozcelik U, Calisir H, Yildirim Z, Gocmen A and Tolun A: Mutations in SLC34A2 cause pulmonary alveolar microlithiasis and are possibly associated with testicular microlithiasis. Am J Hum Genet. 79:650–656. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Izumi S Huqun, Miyazawa H, Ishii K, Uchiyama B, Ishida T, Tanaka S, Tazawa R, Fukuyama S, Tanaka T, et al: Mutations in the SLC34A2 gene are associated with pulmonary alveolar microlithiasis. Am J Respir Crit Care Med. 175:263–268. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Izumi H, Kurai J, Kodani M, Watanabe M, Yamamoto A, Nanba E, Adachi K, Igishi T and Shimizu E: A novel SLC34A2 mutation in a patient with pulmonary alveolar microlithiasis. Hum Genome Var. 4:160472017. View Article : Google Scholar : PubMed/NCBI

10 

Poelma DL, Ju MR, Bakker SC, Zimmermann LJ, Lachmann BF and van Iwaarden JF: A common pathway for the uptake of surfactant lipids by alveolar cells. Am J Respir Cell Mol Biol. 30:751–758. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Dogan OT, Ozsahin SL, Gul E, Arslan S, Koksal B, Berk S, Ozdemir O and Akkurt I: A frame-shift mutation in the SLC34A2 gene in three patients with pulmonary alveolar microlithiasis in an inbred family. Intern Med. 49:45–49. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Yin X, Wang H, Wu D, Zhao G, Shao J and Dai Y: SLC34A2 Gene mutation of pulmonary alveolar microlithiasis: Report of four cases and review of literatures. Respir Med. 107:217–222. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Monabati A, Ghayumi MA and Kumar PV: Familial pulmonary alveolar microlithiasis diagnosed by bronchoalveolar lavage. A case report. Acta Cytol. 51:80–82. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Zhong YQ, Hu CP, Cai XD and Nie HP: A novel mutation of the SLC34A2 gene in a Chinese pedigree with pulmonary alveolar microlithiasis. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 26:365–368. 2009.(In Chinese). PubMed/NCBI

15 

Tachibana T, Hagiwara K and Johkoh T: Pulmonary alveolar microlithiasis: Review and management. Curr Opin Pulm Med. 15:486–490. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Ozcelik U, Yalcin E, Ariyurek M, Ersoz DD, Cinel G, Gulhan B and Kiper N: Long-term results of disodium etidronate treatment in pulmonary alveolar microlithiasis. Pediatr Pulmonol. 45:514–517. 2010.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma T, Qu D, Yan B, Zhang Q, Ren J and Hu Y: Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells. Exp Ther Med 15: 310-314, 2018.
APA
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., & Hu, Y. (2018). Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells. Experimental and Therapeutic Medicine, 15, 310-314. https://doi.org/10.3892/etm.2017.5380
MLA
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., Hu, Y."Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells". Experimental and Therapeutic Medicine 15.1 (2018): 310-314.
Chicago
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., Hu, Y."Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 310-314. https://doi.org/10.3892/etm.2017.5380
Copy and paste a formatted citation
x
Spandidos Publications style
Ma T, Qu D, Yan B, Zhang Q, Ren J and Hu Y: Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells. Exp Ther Med 15: 310-314, 2018.
APA
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., & Hu, Y. (2018). Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells. Experimental and Therapeutic Medicine, 15, 310-314. https://doi.org/10.3892/etm.2017.5380
MLA
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., Hu, Y."Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells". Experimental and Therapeutic Medicine 15.1 (2018): 310-314.
Chicago
Ma, T., Qu, D., Yan, B., Zhang, Q., Ren, J., Hu, Y."Effect of SLC34A2 gene mutation on extracellular phosphorus transport in PAM alveolar epithelial cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 310-314. https://doi.org/10.3892/etm.2017.5380
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