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Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells

  • Authors:
    • Fafeng Cheng
    • Chongyang Ma
    • Liangming Sun
    • Xiaoyu Zhang
    • Changming Zhai
    • Changxiang Li
    • Shuang Zhang
    • Beida Ren
    • Shuling Liu
    • Songnan Liu
    • Xiangjun Yin
    • Xueqian Wang
    • Qingguo Wang
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, Beijing University of Chinese Medicine, School of Basic Medical Sciences, Chao Yang, Beijing 100029, P.R. China, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, P.R. China, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 320-326
    |
    Published online on: October 30, 2017
       https://doi.org/10.3892/etm.2017.5395
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Abstract

Endoplasmic reticulum stress (ERS) and autophagy activation play important roles in the process of cerebral ischemia/reperfusion (I/R) injury. The synergistic protective effects of Geniposide and ursodeoxycholic acid against cellular apoptosis caused by oxygen‑glucose deprivation‑reoxygenation (OGD/R) were investigated using a Cell Counting Kit‑8 assay, lactate dehydrogenase (LDH) assay, flow cytometry, quantitative polymerase chain reaction (qPCR), and western blotting to examine cellular viability, apoptosis, reactive oxygen species (ROS) levels, mRNA and protein levels, respectively, in relation to ERS and autophagy. We found that pretreatment with Geniposide improved cellular viability. Moreover, treatment with a combination of Geniposide and Tauroursodeoxycholic acid (TUDCA) (GT) protected injured cells better than Geniposide alone. Further studies showed that the increase in cellular ROS levels, and the overexpression of mRNA and proteins related to OGD/R‑induced ERS and autophagy, were both counteracted by GT. Our study indicates that the protective effects of GT on OGD/R‑induced apoptosis in SH‑SY5Y cells are associated with the inhibition of ERS and autophagy.
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1 

Fan YY, Hu WW, Nan F and Chen Z: Postconditioning-induced neuroprotection, mechanisms and applications in cerebral ischemia. Neurochem Int. 107:43–56. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Portt L, Norman G, Clapp C, Greenwood M and Greenwood MT: Anti-apoptosis and cell survival: A review. Biochim Biophys Acta. 1813:238–259. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kong FJ, Wu JH, Sun SY and Zhou JQ: The endoplasmic reticulum stress/autophagy pathway is involved in cholesterol-induced pancreatic β-cell injury. Sci Rep. 7:447462017. View Article : Google Scholar : PubMed/NCBI

4 

Cheng F, Zhong X, Lu Y, Wang X, Song W, Guo S, Wang X, Liu D and Wang Q: Refined qingkailing protects MCAO mice from endoplasmic reticulum stress-induced apoptosis with a broad time window. Evid Based Complement Alternat Med. 2012:5678722012. View Article : Google Scholar : PubMed/NCBI

5 

Xu XL, Ma CY, Wang XQ, Wang GL, Zhai CM, Yue WC, Li CX, Zhang XY, Shen XD, Mu J, et al: Comparative study of cholic acid compounds of bezoar on anti-cerebral infarction and regulating endoplasmic reticulum stress. Drug Evaluation Res. 40:11–19. 2017.(In Chinese).

6 

Cao K and Tong CW: Research progress of pharmacological effects and application of pine needle. J Mianyang Norm Univ. 2015.(In Chinese).

7 

Li C, Pan Y and Jia X: Effects of Huangqin (dried root of scutellaria baicalensis) and Zhizi (dried fruit of Gardenia jasminoides) used in combination on ischemic cascade reaction in the rat model of focal cerebral ischemia and reperfusion. J Beijing Univ Tradit Chin Med. 2002.

8 

Liu J, Yin F, Zheng X, Jing J and Hu Y: Geniposide, a novel agonist for GLP-1 receptor, prevents PC12 cells from oxidative damage via MAP kinase pathway. Neurochem Int. 51:361–369. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Zhao C, Lv C, Li H, Du S, Liu X, Li Z, Xin W and Zhang W: Geniposide protects primary cortical neurons against oligomeric Aβ1-42-induced neurotoxicity through a mitochondrial pathway. PLoS One. 11:e01525512016. View Article : Google Scholar : PubMed/NCBI

10 

Lv C, Wang L, Liu X, Yan S, Yan SS, Wang Y and Zhang W: Multi-faced neuroprotective effects of geniposide depending on the RAGE-mediated signaling in an Alzheimer mouse model. Neuropharmacology. 89:175–184. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Keene CD, Rodrigues CM, Eich T, Chhabra MS, Steer CJ and Low WC: Tauroursodeoxycholic acid, a bile acid, is neuroprotective in a transgenic animal model of Huntington's disease. Proc Natl Acad Sci USA. 99:pp. 10671–10676. 2002; View Article : Google Scholar : PubMed/NCBI

12 

Rodrigues CM, Sola S, Nan Z, Castro RE, Ribeiro PS, Low WC and Steer CJ: Tauroursodeoxycholic acid reduces apoptosis and protects against neurological injury after acute hemorrhagic stroke in rats. Proc Natl Acad Sci USA. 100:pp. 6087–6092. 2003; View Article : Google Scholar : PubMed/NCBI

13 

Fernández-Sánchez L, Lax P, Pinilla I, Martín-Nieto J and Cuenca N: Tauroursodeoxycholic acid prevents retinal degeneration in transgenic P23H rats. Invest Ophthalmol Vis Sci. 52:4998–5008. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Rodrigues CM, Spellman SR, Solá S, Grande AW, Linehan-Stieers C, Low WC and Steer CJ: Neuroprotection by a bile acid in an acute stroke model in the rat. J Cereb Blood Flow Metab. 22:463–471. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Amaral JD, Viana RJ, Ramalho RM, Steer CJ and Rodrigues CM: Bile acids: Regulation of apoptosis by ursodeoxycholic acid. J Lipid Res. 50:1721–1734. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Gaspar JM, Martins A, Cruz R, Rodrigues CM, Ambrósio AF and Santiago AR: Tauroursodeoxycholic acid protects retinal neural cells from cell death induced by prolonged exposure to elevated glucose. Neuroscience. 253:380–388. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zhong XM, Ren XC, Lou YL, Chen MJ, Li GZ, Gong XY and Huang Z: Effects of in-vitro cultured calculus bovis on learning and memory impairments of hyperlipemia vascular dementia rats. J Ethnopharmacol. 192:390–397. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Fu WJ, Lei T, Yin Z, Pan JH, Chai YS, Xu XY, Yan YX, Wang ZH, Ke J, Wu G, et al: Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. J Ethnopharmacol. 195:118–126. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Wang J, Hou J, Lei H, Fu J, Pan Y and Liu J: Synergistic neuroprotective effect of microglial-conditioned media treated with geniposide and ginsenoside Rg1 on hypoxia injured neurons. Mol Med Rep. 12:5328–5334. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Jiang YQ, Chang GL, Wang Y, Zhang DY, Cao L and Liu J: Geniposide prevents hypoxia/reoxygenation-induced apoptosis in H9c2 cells: Improvement of mitochondrial dysfunction and activation of GLP-1R and the PI3K/AKT signaling pathway. Cell Physiol Biochem. 39:407–421. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Guo LX, Liu JH, Zheng XX, Yin ZY, Kosaraju J and Tam KY: Geniposide improves insulin production and reduces apoptosis in high glucose-induced glucotoxic insulinoma cells. Eur J Pharm Sci. pii:S0928-0987(17)30173-2. 2017.

22 

Li L, Zhao J, Liu K, Li GL, Han YQ and Liu YZ: Geniposide prevents rotenone-induced apoptosis in primary cultured neurons. Neural Regen Res. 10:1617–1621. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Gordeziani M, Adamia G, Khatisashvili G and Gigolashvili G: Programmed cell self-liquidation (apoptosis). Ann Agrarian Sci. 15:148–154. 2017. View Article : Google Scholar

24 

Narne P, Pandey V and Phanithi PB: Interplay between mitochondrial metabolism and oxidative stress in ischemic stroke: An epigenetic connection. Mol Cell Neurosci. 82:176–194. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Wu TS, Liao YC, Yu FY, Chang CH and Liu BH: Mechanism of patulin-induced apoptosis in human leukemia cells (HL-60). Toxicol Lett. 183:105–111. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Ogen-Shtern N, Ben David T and Lederkremer GZ: Protein aggregation and ER stress. Brain Res. 1648:658–666. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Senft D and Ronai ZA: UPR, autophagy, and mitochondria crosstalk underlies the ER stress response. Trends Biochem Sci. 40:141–148. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Towers CG and Thorburn A: Therapeutic targeting of autophagy. EBioMedicine. 14:15–23. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Maejima Y, Isobe M and Sadoshima J: Regulation of autophagy by beclin 1 in the heart. J Mol Cell Cardiol. 95:19–25. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cheng F, Ma C, Sun L, Zhang X, Zhai C, Li C, Zhang S, Ren B, Liu S, Liu S, Liu S, et al: Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells. Exp Ther Med 15: 320-326, 2018.
APA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C. ... Wang, Q. (2018). Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells. Experimental and Therapeutic Medicine, 15, 320-326. https://doi.org/10.3892/etm.2017.5395
MLA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15.1 (2018): 320-326.
Chicago
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 320-326. https://doi.org/10.3892/etm.2017.5395
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng F, Ma C, Sun L, Zhang X, Zhai C, Li C, Zhang S, Ren B, Liu S, Liu S, Liu S, et al: Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells. Exp Ther Med 15: 320-326, 2018.
APA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C. ... Wang, Q. (2018). Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells. Experimental and Therapeutic Medicine, 15, 320-326. https://doi.org/10.3892/etm.2017.5395
MLA
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15.1 (2018): 320-326.
Chicago
Cheng, F., Ma, C., Sun, L., Zhang, X., Zhai, C., Li, C., Zhang, S., Ren, B., Liu, S., Liu, S., Yin, X., Wang, X., Wang, Q."Synergistic neuroprotective effects of Geniposide and ursodeoxycholic acid in hypoxia‑reoxygenation injury in SH‑SY5Y cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 320-326. https://doi.org/10.3892/etm.2017.5395
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