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Article

Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes

  • Authors:
    • Xiaoliu Liu
    • Sufang Liu
    • Jian Lei
    • Lixin Zou
    • Le Xiao
    • Guangsen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The Affiliated Changsha Hospital, Hunan Normal University, Changsha, Hunan 410006, P.R. China, Division of Hematology, Institute of Molecular Hematology, The Second Xiang‑Ya Hospital, Central South University, Changsha, Hunan 410011, P.R. China, Department of Pathology, The Affiliated Tumor Hospital, Xiangya School of Medicine, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 500-505
    |
    Published online on: October 30, 2017
       https://doi.org/10.3892/etm.2017.5402
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Abstract

As a highly heterogeneous disease, the pathogenesis of myelodysplastic syndrome (MDS) has not been well defined. In the present study, human mutS homolog 2 (hMSH2) promoter methylation was detected with methylation‑specific polymerase chain reaction (PCR). The function of hMSH2 was analyzed by microsatellite instability (MSI) detection of BAT‑26, and hMSH2 expression was evaluated using reverse transcription‑quantitative PCR in 60 patients with MDS. The results revealed methylation of the hMSH2 promoter in 18 patients with MDS who have an overall prevalence of 30% (95% confidence interval, 18.4‑41.6%). Among the patients with hMSH2 methylation, 2 patients exhibited MSI. It was demonstrated that hMSH2 promoter methylation was increased in MDS with an increase in Revised International Prognostic Scoring System (IPSS‑R) risk, and patients with higher hMSH2 promoter methylation had shorter overall survival by Kaplan‑Meier analysis (P=0.011). In addition, it was also observed that decreased hMSH2 mRNA expression was associated with high IPSS‑R risk group (high/very high vs. intermediate, P=0.003), and hMSH2 mRNA expression in CD34 positive bone marrow cells was lower compared with that in CD34 negative cells of patients with MDS (P=0.029). Methylation of hMSH2 may be valuable for prognostic evaluation and progression prediction of MDS. Furthermore, hMSH2 may serve a key function in the pathogenesis and prognosis of MDS.
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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Liu S, Lei J, Zou L, Xiao L and Zhang G: Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes. Exp Ther Med 15: 500-505, 2018.
APA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., & Zhang, G. (2018). Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes. Experimental and Therapeutic Medicine, 15, 500-505. https://doi.org/10.3892/etm.2017.5402
MLA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15.1 (2018): 500-505.
Chicago
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15, no. 1 (2018): 500-505. https://doi.org/10.3892/etm.2017.5402
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Liu S, Lei J, Zou L, Xiao L and Zhang G: Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes. Exp Ther Med 15: 500-505, 2018.
APA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., & Zhang, G. (2018). Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes. Experimental and Therapeutic Medicine, 15, 500-505. https://doi.org/10.3892/etm.2017.5402
MLA
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15.1 (2018): 500-505.
Chicago
Liu, X., Liu, S., Lei, J., Zou, L., Xiao, L., Zhang, G."Methylation and expression of mismatch repair gene human mutS homolog 2 in myelodysplastic syndromes". Experimental and Therapeutic Medicine 15, no. 1 (2018): 500-505. https://doi.org/10.3892/etm.2017.5402
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