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MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures

  • Authors:
    • Kai‑Bao Ji
    • Ling Ling
    • Qian Zhang
    • Jing‑Jing Chou
    • Xia‑Ling Yang
    • Zhi‑Hong Wang
    • Li Yin
    • Shu‑Fang Wu
    • Yi‑Feng Yu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Affiliated Eye Hospital of Nanchang University, Jiangxi Provincial Key Laboratory for Ophthalmology, Nanchang, Jiangxi 330006, P.R. China , Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 139-144
    |
    Published online on: October 31, 2017
       https://doi.org/10.3892/etm.2017.5408
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Abstract

Alkali burns of the cornea may lead to permanent visual impairment or complete blindness. In the current study, the role of microRNA 296 (miR‑296) was explored in mouse corneal neovascularization induced by alkali burns. An alkali burn model in Balb/c mice was developed to study chemical corneal injuries. The expression of the miR‑296 gene was measured by reverse‑transcription‑quantitative polymerase chain reaction. Fibroblast growth factor 23 (FGF23) protein expression was measured by western blot analysis. Possible impacted pathways were analyzed by Kyoto Encyclopedia of Genes and Genomes pathway analysis. miR‑296 gene expression was examined following chemical corneal injury and it was demonstrated that different topical eye medications decreased miR‑296 gene expression. miR‑296 may participate in cytokine‑cytokine receptor interaction pathways to influence corneal inflammatory responses. It was also revealed that FGF23 was expressed following chemical corneal injury and that different treatments with topical eye drops decreased its expression. miR‑296 is a novel molecular modulator for alkali burns in the mouse cornea.
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1 

Xie Y, Tan Y and Tang S: Epidemiology of 377 patients with chemical burns in Guangdong province. Burns. 30:569–572. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Zhang C and Xu J: Indications for penetrating keratoplasty in East China, 1994–2003. Graefes Arch Clin Exp Ophthalmol. 243:1005–1009. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Hong J, Qiu T, Wei A, Sun X and Xu J: Clinical characteristics and visual outcome of severe ocular chemical injuries in Shanghai. Ophthalmology. 117:2268–2272. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Saika S, Kobata S, Hashizume N, Okada Y and Yamanaka O: Epithelial basement membrane in alkali-burned corneas in rats. Immunohistochemical study. Cornea. 12:383–390. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Ishizaki M, Zhu G, Haseba T, Shafer SS and Kao WW: Expression of collagen I, smooth muscle alpha-actin, and vimentin during the healing of alkali-burned and lacerated corneas. Invest Ophthalmol Vis Sci. 34:3320–3328. 1993.PubMed/NCBI

6 

Pandya NM, Dhalla NS and Santani DD: Angiogenesis-a new target for future therapy. Vascul Pharmacol. 44:265–274. 2006. View Article : Google Scholar : PubMed/NCBI

7 

de Groot H, Schmit-Eilenberger V, Kirchhof J and Augustin AJ: Angiostatic and angiogenic factors. Dev Ophthalmol. 46:1–3. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Ranganathan K and Sivasankar V: MicroRNAs-Biology and clinical applications. J Oral Maxillofac Pathol. 18:229–234. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Ryan DG, Oliveira-Fernandes M and Lavker RM: MicroRNAs of the mammalian eye display distinct and overlapping tissue specificity. Mol Vis. 12:1175–1184. 2006.PubMed/NCBI

11 

Karali M, Peluso I, Gennarino VA, Bilio M, Verde R, Lago G, Dollé P and Banfi S: miRNeye: A microRNA expression atlas of the mouse eye. BMC Genomics. 11:7152010. View Article : Google Scholar : PubMed/NCBI

12 

Würdinger T, Tannous BA, Saydam O, Skog J, Grau S, Soutschek J, Weissleder R, Breakefield XO and Krichevsky AM: miR-296 regulates growth factor receptor overexpression in angiogenic endothelial cells. Cancer Cell. 14:382–393. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Wei JJ, Wu X, Peng Y, Shi G, Basturk O, Yang X, Daniels G, Osman I, Ouyang J, Hernando E, et al: Regulation of HMGA1 expression by microRNA-296 affects prostate cancer growth and invasion. Clin Cancer Res. 17:1297–1305. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Feng J, Huang T, Huang Q, Chen H, Li Y, He W, Wang GB, Zhang L, Xia J, Zhang N and Liu Y: Pro-angiogenic microRNA-296 upregulates vascular endothelial growth factor and downregulates Notch1 following cerebral ischemic injury. Mol Med Rep. 12:8141–8147. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Bian F, Pelegrino FS, Henriksson JT, Pflugfelder SC, Volpe EA, Li DQ and de Paiva CS: Differential effects of dexamethasone and doxycycline on inflammation and MMP production in alkali-burned corneas associated with dry eye. Ocul Surf. 14:242–254. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Draghici S, Khatri P, Tarca AL, Amin K, Done A, Voichita C, Georgescu C and Romero R: A systems biology approach for pathway level analysis. Genome Res. 17:1537–1545. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Kanehisa M, Goto S, Kawashima S, Okuno Y and Hattori M: The KEGG resource for deciphering the genome. Nucleic Acids Res. 32(Database issue): D277–D280. 2004. View Article : Google Scholar : PubMed/NCBI

18 

Du J, Li M, Yuan Z, Guo M, Song J, Xie X and Chen Y: A decision analysis model for KEGG pathway analysis. BMC Bioinformatics. 17:4072016. View Article : Google Scholar : PubMed/NCBI

19 

Kanehisa M, Sato Y, Kawashima M, Furumichi M and Tanabe M: KEGG as a reference resource for gene and protein annotation. Nucleic Acids Res. 44:D457–D462. 2016. View Article : Google Scholar : PubMed/NCBI

20 

Khatri P, Sirota M and Butte AJ: Ten years of pathway analysis: Current approaches and outstanding challenges. PLoS Comput Biol. 8:e10023752012. View Article : Google Scholar : PubMed/NCBI

21 

Wilson SE, Mohan RR, Mohan RR, Ambrósio R Jr, Hong J and Lee J: The corneal wound healing response: Cytokine-mediated interaction of the epithelium, stroma, and inflammatory cells. Prog Retin Eye Res. 20:625–637. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Park JH, Joo CK and Chung SK: Comparative study of tacrolimus and bevacizumab on corneal neovascularization in rabbits. Cornea. 34:449–455. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Nakao S, Hata Y, Miura M, Noda K, Kimura YN, Kawahara S, Kita T, Hisatomi T, Nakazawa T, Jin Y, et al: Dexamethasone inhibits interleukin-1beta-induced corneal neovascularization: Role of nuclear factor-kappaB-activated stromal cells in inflammatory angiogenesis. Am J Pathol. 171:1058–1065. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Nakao S, Zandi S, Lara-Castillo N, Taher M, Ishibashi T and Hafezi-Moghadam A: Larger therapeutic window for steroid versus VEGF-A inhibitor in inflammatory angiogenesis: Surprisingly similar impact on leukocyte infiltration. Invest Ophthalmol Vis Sci. 53:3296–3302. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Yuan J, Zhai JJ, Chen JQ, Ye CT and Zhou SY: Preparation of 0.05% FK506 suspension eyedrops and its pharmacokinetics after topical ocular administration. J Ocul Pharmacol Ther. 25:345–350. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ji KB, Ling L, Zhang Q, Chou JJ, Yang XL, Wang ZH, Yin L, Wu SF and Yu YF: MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures. Exp Ther Med 15: 139-144, 2018.
APA
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z. ... Yu, Y. (2018). MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures. Experimental and Therapeutic Medicine, 15, 139-144. https://doi.org/10.3892/etm.2017.5408
MLA
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z., Yin, L., Wu, S., Yu, Y."MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures". Experimental and Therapeutic Medicine 15.1 (2018): 139-144.
Chicago
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z., Yin, L., Wu, S., Yu, Y."MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures". Experimental and Therapeutic Medicine 15, no. 1 (2018): 139-144. https://doi.org/10.3892/etm.2017.5408
Copy and paste a formatted citation
x
Spandidos Publications style
Ji KB, Ling L, Zhang Q, Chou JJ, Yang XL, Wang ZH, Yin L, Wu SF and Yu YF: MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures. Exp Ther Med 15: 139-144, 2018.
APA
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z. ... Yu, Y. (2018). MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures. Experimental and Therapeutic Medicine, 15, 139-144. https://doi.org/10.3892/etm.2017.5408
MLA
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z., Yin, L., Wu, S., Yu, Y."MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures". Experimental and Therapeutic Medicine 15.1 (2018): 139-144.
Chicago
Ji, K., Ling, L., Zhang, Q., Chou, J., Yang, X., Wang, Z., Yin, L., Wu, S., Yu, Y."MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures". Experimental and Therapeutic Medicine 15, no. 1 (2018): 139-144. https://doi.org/10.3892/etm.2017.5408
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