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Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model

  • Authors:
    • Yongjie Yuan
    • Si Yang
    • Chao Li
    • Kan Xu
    • Jinlu Yu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
    Copyright: © Yuan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 145-151
    |
    Published online on: November 1, 2017
       https://doi.org/10.3892/etm.2017.5423
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Abstract

In the present study, a recombinant adeno‑associated virus vector containing the calcitonin gene related peptide gene (rAAV‑CGRP) was constructed and the therapeutic effect of rAAV‑CGRP on a chick umbilical artery vasospasm model induced by chick embryo allantoic cavity hemorrhage was investigated. Fresh specific pathogen‑free fertilized chicken eggs were randomly divided into a rAAV‑CGRP group, an empty vector virus (AAV) group, and a control group, with 24 eggs in each group. An umbilical arterial vasospasm model was established using a needle puncture method on a vein in the chorioallantoic membrane to induce a hemorrhage in the allantoic cavity of 11‑day‑old chicken embryonated eggs. A total of 24 h after model establishment, 1 ml of rAAV‑CGRP and empty vector virus solution of rAAV‑CGRP and empty vector virus solution was, respectively, injected into the allantoic cavity in the rAAV‑CGRP and AAV groups. Experimental results showed that after 72 h of model establishment, the mortality rates of the 3‑, 5‑ and 7‑day subgroups in the rAAV‑CGRP group were lower than in the subgroups of the AAV injection group. After 3, 5 and 7 days of model establishment in the rAAV‑CGRP group, the cross‑sectional area of the inner diameter of the umbilical arteries was larger than that of the AAV group; the vessel wall thicknesses of the rAAV‑CGRP group were thinner than in the AAV group. In addition, the concentration of CGRP in chick embryo allantoic fluid significantly increased and was several times higher than in the AAV group (P<0.05). In conclusion, administration of rAAV‑CGRP through the allantoic cavity may increase the viability of a vasospasm model induced by chick allantoic cavity hemorrhage, significantly improve umbilical artery vasospasm, and increase CGRP expression in the chick embryo allantoic cavity. This approach also provides a novel experimental model for identifying other target genes for the gene therapy of vasospasm.
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1 

Becker KJ: Epidemiology and clinical presentation of aneurysmal subarachnoid hemorrhage. Neurosurg Clin N Am. 9:435–444. 1998.PubMed/NCBI

2 

Przybycien-Szymanska MM and Ashley WW Jr: Biomarker discovery in cerebral vasospasm after aneurysmal subarachnoid hemorrhage. J Stroke Cerebrovasc Dis. 24:1453–1464. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Budohoski KP, Guilfoyle M, Helmy A, Huuskonen T, Czosnyka M, Kirollos R, Menon DK, Pickard JD and Kirkpatrick PJ: The pathophysiology and treatment of delayed cerebral ischaemia following subarachnoid haemorrhage. J Neurol Neurosurg Psychiatry. 85:1343–1353. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Gathier CS, van den Bergh WM and Slooter AJ; HIMALAIA-Study Group, : HIMALAIA (Hypertension Induction in the Management of AneurysmaL subArachnoid haemorrhage with secondary IschaemiA): A randomized single-blind controlled trial of induced hypertension vs. no induced hypertension in the treatment of delayed cerebral ischemia after subarachnoid hemorrhage. Int J Stroke. 9:375–380. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Francoeur CL and Mayer SA: Management of delayed cerebral ischemia after subarachnoid hemorrhage. Crit Care. 20:2772016. View Article : Google Scholar : PubMed/NCBI

6 

Sun BL, Shen FP, Wu QJ, Chi SM, Yang MF, Yuan H, Xie FM, Zhang YB, Chen J and Zhang F: Intranasal delivery of calcitonin gene-related peptide reduces cerebral vasospasm in rats. Front Biosci (Elite Ed). 2:1502–1513. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Tian XH, Wang ZG, Meng H, Wang YH, Feng W, Wei F, Huang ZC, Lin XN and Ren L: Tat peptide-decorated gelatin-siloxane nanoparticles for delivery of CGRP transgene in treatment of cerebral vasospasm. Int J Nanomedicine. 8:865–876. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Kolias AG, Sen J and Belli A: Pathogenesis of cerebral vasospasm following aneurysmal subarachnoid hemorrhage: Putative mechanisms and novel approaches. J Neurosci Res. 87:1–11. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Hu N, Wu Y, Chen BZ, Han JF and Zhou MT: Protective effect of stellate ganglion block on delayed cerebral vasospasm in an experimental rat model of subarachnoid hemorrhage. Brain Res. 1585:63–71. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Nozaki K, Uemura Y, Okamoto S, Kikuchi H and Mizuno N: Relaxant effect of calcitonin gene-related peptide on cerebral arterial spasm induced by experimental subarachnoid hemorrhage in dogs. J Neurosurg. 71:558–564. 1989. View Article : Google Scholar : PubMed/NCBI

11 

Toshima M, Kassell NF, Tanaka Y and Dougherty DA: Effect of intracisternal and intravenous calcitonin gene-related peptide on experimental cerebral vasospasm in rabbits. Acta Neurochir (Wien). 119:134–138. 1992. View Article : Google Scholar : PubMed/NCBI

12 

Juul R, Aakhus S, Bjornstad K, Gisvold SE, Brubakk AO and Edvinsson L: Calcitonin gene-related peptide (human alpha-CGRP) counteracts vasoconstriction in human subarachnoid haemorrhage. Neurosci Lett. 170:67–70. 1994. View Article : Google Scholar : PubMed/NCBI

13 

Juul R, Edvinsson L, Gisvold SE, Ekman R, Brubakk AO and Fredriksen TA: Calcitonin gene-related peptide-LI in subarachnoid haemorrhage in man. Signs of activation of the trigemino-cerebrovascular system? Br J Neurosurg. 4:171–179. 1990.PubMed/NCBI

14 

Effect of calcitonin-gene-related peptide in patients with delayed postoperative cerebral ischaemia after aneurysmal subarachnoid haemorrhage. European CGRP in Subarachnoid Haemorrhage Study Group. Lancet. 339:831–834. 1992.PubMed/NCBI

15 

Kokkoris S, Andrews P and Webb DJ: Role of calcitonin gene-related peptide in cerebral vasospasm, and as a therapeutic approach to subarachnoid hemorrhage. Front Endocrinol (Lausanne). 3:1352012.PubMed/NCBI

16 

Yuan Y, Yang S, Li C, Xu K, Luo Q and Yu J: The chicken embryo umbilical artery is a promising in vivo model system for the study of vasospasm. Int J Clin Exp Med. 9:1139–1149. 2016.

17 

Pallás V, Más P and Sánchez-Navarro JA: Detection of plant RNA viruses by nonisotopic dot-blot hybridization. Methods Mol Biol. 81:461–468. 1998.PubMed/NCBI

18 

Edvinsson L, Ekman R, Jansen I, McCulloch J, Mortensen A and Uddman R: Reduced levels of calcitonin gene-related peptide-like immunoreactivity in human brain vessels after subarachnoid haemorrhage. Neurosci Lett. 121:151–154. 1991. View Article : Google Scholar : PubMed/NCBI

19 

Schebesch KM, Herbst A, Bele S, Schödel P, Brawanski A, Stoerr EM, Lohmeier A, Kagerbauer SM, Martin J and Proescholdt M: Calcitonin-gene related peptide and cerebral vasospasm. J Clin Neurosci. 20:584–586. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Juul R, Hara H, Gisvold SE, Brubakk AO, Fredriksen TA, Waldemar G, Schmidt JF, Ekman R and Edvinsson L: Alterations in perivascular dilatory neuropeptides (CGRP, SP, VIP) in the external jugular vein and in the cerebrospinal fluid following subarachnoid haemorrhage in man. Acta Neurochir (Wien). 132:32–41. 1995. View Article : Google Scholar : PubMed/NCBI

21 

Edvinsson L, Delgado-Zygmunt T, Ekman R, Jansen I, Svendgaard NA and Uddman R: Involvement of perivascular sensory fibers in the pathophysiology of cerebral vasospasm following subarachnoid hemorrhage. J Cereb Blood Flow Metab. 10:602–607. 1990. View Article : Google Scholar : PubMed/NCBI

22 

Imaizumi S, Shimizu H, Ahmad I, Kaminuma T, Tajima M and Yoshimoto T: Effect of calcitonin gene-related peptide on delayed cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits. Surg Neurol. 46:263–271. 1996. View Article : Google Scholar : PubMed/NCBI

23 

Ahmad I, Imaizumi S, Shimizu H, Kaminuma T, Ochiai N, Tajima M and Yoshimoto T: Development of calcitonin gene-related peptide slow-release tablet implanted in CSF space for prevention of cerebral vasospasm after experimental subarachnoid haemorrhage. Acta Neurochir (Wien). 138:1230–1240. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Inoue T, Shimizu H, Kaminuma T, Tajima M, Watabe K and Yoshimoto T: Prevention of cerebral vasospasm by calcitonin gene-related peptide slow-release tablet after subarachnoid hemorrhage in monkeys. Neurosurgery. 39:984–990. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Toyoda K, Faraci FM, Watanabe Y, Ueda T, Andresen JJ, Chu Y, Otake S and Heistad DD: Gene transfer of calcitonin gene-related peptide prevents vasoconstriction after subarachnoid hemorrhage. Circ Res. 87:818–824. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Satoh M, Perkins E, Kimura H, Tang J, Chun Y, Heistad DD and Zhang JH: Posttreatment with adenovirus-mediated gene transfer of calcitonin gene-related peptide to reverse cerebral vasospasm in dogs. J Neurosurg. 97:136–142. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Yuan YJ, Xu K, Wu W, Luo Q and Yu JL: Application of the chick embryo chorioallantoic membrane in neurosurgery disease. Int J Med Sci. 11:1275–1281. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Tufan AC and Satiroglu-Tufan NL: The chick embryo chorioallantoic membrane as a model system for the study of tumor angiogenesis, invasion and development of anti-angiogenic agents. Curr Cancer Drug Targets. 5:249–266. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Tutykhina IL, Bezborodova OA, Shmarov MM, Logunov DY, Neugodova GL, Nemtsova ER, Naroditsky BS, Yakubovskaya RI and Gintsburg AL: Production of recombinant human lactoferrin in the allantoic fluid of embryonated chicken eggs and its characteristics. Protein Expr Purif. 65:100–107. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Wang Y, Sun H, Shen P, Zhang X and Xia X: Effective inhibition of infectious bursal disease virus replication by recombinant avian adeno-associated virus-delivered microRNAs. J Gen Virol. 90:1417–1422. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Ribatti D: Chicken chorioallantoic membrane angiogenesis model. Methods Mol Biol. 843:47–57. 2012. View Article : Google Scholar : PubMed/NCBI

32 

Palmer TD, Lewis J and Zijlstra A: Quantitative analysis of cancer metastasis using an avian embryo model. J Vis Exp. 28152011.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yuan Y, Yang S, Li C, Xu K and Yu J: Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model. Exp Ther Med 15: 145-151, 2018.
APA
Yuan, Y., Yang, S., Li, C., Xu, K., & Yu, J. (2018). Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model. Experimental and Therapeutic Medicine, 15, 145-151. https://doi.org/10.3892/etm.2017.5423
MLA
Yuan, Y., Yang, S., Li, C., Xu, K., Yu, J."Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model". Experimental and Therapeutic Medicine 15.1 (2018): 145-151.
Chicago
Yuan, Y., Yang, S., Li, C., Xu, K., Yu, J."Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model". Experimental and Therapeutic Medicine 15, no. 1 (2018): 145-151. https://doi.org/10.3892/etm.2017.5423
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Y, Yang S, Li C, Xu K and Yu J: Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model. Exp Ther Med 15: 145-151, 2018.
APA
Yuan, Y., Yang, S., Li, C., Xu, K., & Yu, J. (2018). Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model. Experimental and Therapeutic Medicine, 15, 145-151. https://doi.org/10.3892/etm.2017.5423
MLA
Yuan, Y., Yang, S., Li, C., Xu, K., Yu, J."Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model". Experimental and Therapeutic Medicine 15.1 (2018): 145-151.
Chicago
Yuan, Y., Yang, S., Li, C., Xu, K., Yu, J."Effect of recombinant adeno‑associated virus expressing calcitonin gene‑related peptide on chick embryo umbilical artery vasospasm model". Experimental and Therapeutic Medicine 15, no. 1 (2018): 145-151. https://doi.org/10.3892/etm.2017.5423
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