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Article Open Access

Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model

  • Authors:
    • Jun Komatsu
    • Nana Nagura
    • Hideaki Iwase
    • Mamoru Igarashi
    • Osamu Ohbayashi
    • Isao Nagaoka
    • Kazuo Kaneko
  • View Affiliations / Copyright

    Affiliations: Department of Medicine for Motor Organs, Juntendo University Graduate School of Medicine, Tokyo 113‑8421, Japan, Department of Bio‑Engineering, Juntendo University Institute of Casualty Center, Izunokuni, Shizuoka 410‑2295, Japan, Department of Host Defense and Biochemical Research, Juntendo University Graduate School of Medicine, Tokyo 113‑8421, Japan, Department of Orthopaedic Surgery, Juntendo University Shizuoka Hospital, Izunokuni, Shizuoka 410‑2295, Japan
    Copyright: © Komatsu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 19-30
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    Published online on: November 1, 2017
       https://doi.org/10.3892/etm.2017.5424
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Abstract

Grafting β‑tricalcium phosphate (TCP) is a well‑established method for restoring bone defects; however, there is concern that the mechanical stability of the grafted β‑TCP is not maintained during bone translation. Teriparatide has an anabolic effect, stimulating bone formation and increasing bone mineral density for the treatment of osteoporosis. The aim of the present study was to evaluate the effect of intermittent teriparatide treatment on changes in bone grafted with β‑TCP using a rabbit bone defect model. Bone defects (5x15 mm) were created in the distal femoral condyle of Japanese white rabbits, and β‑TCP granules of two different total porosities were manually grafted. Teriparatide (40 µg/kg) or 0.2% rabbit serum albumin solution as a vehicle control was subcutaneously injected three times per week following the surgery. At 4 or 8 weeks post‑surgery, serum samples were obtained and the levels of γ‑carboxylated osteocalcin (Gla‑OC) were quantified using ELISA. Histomorphometry was also performed using sections of graft sites following staining for tartrate resistant acid phosphatase. Activity and mechanical strength (maximum shear strength, maximum shear stiffness and total energy absorption) were evaluated using an axial push‑out load to failure test. Teriparatide treatment significantly increased (P<0.05) the serum levels of Gla‑OC, a specific marker for bone formation, suggesting that teriparatide enhances bone formation in β‑TCP‑grafted rabbits. Furthermore teriparatide increased the degradation of β‑TCP by bone remodeling (P<0.05) and promoted the formation of new bone following application of the graft compared with the control group (P<0.01). Furthermore, teriparatide suppressed the reduction in mechanical strength (P<0.05) during bone translation in bone defects grafted with β‑TCP. The results of the present study demonstrate that teriparatide is effective in maintaining the mechanical stability of grafted β‑TCP, possibly by promoting new bone formation.
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Copy and paste a formatted citation
Spandidos Publications style
Komatsu J, Nagura N, Iwase H, Igarashi M, Ohbayashi O, Nagaoka I and Kaneko K: Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model. Exp Ther Med 15: 19-30, 2018.
APA
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., & Kaneko, K. (2018). Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model. Experimental and Therapeutic Medicine, 15, 19-30. https://doi.org/10.3892/etm.2017.5424
MLA
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., Kaneko, K."Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model". Experimental and Therapeutic Medicine 15.1 (2018): 19-30.
Chicago
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., Kaneko, K."Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model". Experimental and Therapeutic Medicine 15, no. 1 (2018): 19-30. https://doi.org/10.3892/etm.2017.5424
Copy and paste a formatted citation
x
Spandidos Publications style
Komatsu J, Nagura N, Iwase H, Igarashi M, Ohbayashi O, Nagaoka I and Kaneko K: Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model. Exp Ther Med 15: 19-30, 2018.
APA
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., & Kaneko, K. (2018). Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model. Experimental and Therapeutic Medicine, 15, 19-30. https://doi.org/10.3892/etm.2017.5424
MLA
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., Kaneko, K."Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model". Experimental and Therapeutic Medicine 15.1 (2018): 19-30.
Chicago
Komatsu, J., Nagura, N., Iwase, H., Igarashi, M., Ohbayashi, O., Nagaoka, I., Kaneko, K."Effect of intermittent administration of teriparatide on the mechanical and histological changes in bone grafted with β‑tricalcium phosphate using a rabbit bone defect model". Experimental and Therapeutic Medicine 15, no. 1 (2018): 19-30. https://doi.org/10.3892/etm.2017.5424
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