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Article Open Access

MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie

  • Authors:
    • Shuichuan Huang
    • Zhibo Chen
    • Weibin Wu
    • Mian Wang
    • Rui Wang
    • Jin Cui
    • Wen Li
    • Shenming Wang
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Surgery, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Vascular Surgery, Sun Yat‑Sen Memorial Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Vascular Surgery, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. ChinaDepartment of Vascular Surgery, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China, Laboratory of General Surgery, The First Affiliated Hospital, Sun Yat‑Sen University, Guangzhou, Guangdong 510080, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 633-640
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/etm.2017.5453
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Abstract

MicroRNA (miR)‑31 serves a key role in various biological processes, including tumor development, angiogenesis and inflammation. Whether miR‑31 is involved in the pathological processes of arteriosclerosis obliterans (ASO) remains to be elucidated, as does the mechanism of miR‑31 regulation of arterial smooth muscle cells (ASMCs). In the present study, miR‑31 expression was detected by reverse transcription‑quantitative polymerase chain reaction and in situ hybridization, and was significantly upregulated in human ASO arterial walls compared with normal arterial walls (P<0.001). In addition, miR‑31 proliferation was detected by Cell Counting Kit‑8 and EdU assays; proliferation was significantly promoted in platelet‑derived growth factor (PDGF)‑BB‑induced human ASMCs (HASMCs) (P<0.001). miR‑31 migration was detected by transwell and wound closure assays, and was revealed to be promoted in PDGF‑BB‑induced HASMCs (P<0.001). Lastly, HASMCs were transfected with miR‑31 mimics and inhibitors, and negative controls. A dual‑luciferase reporter assay was performed to verify that mitofusin‑2 (MFN2) was a direct target of miR‑31 and that MFN2 expression was significantly downregulated by miR‑31 at a post‑transcriptional level in HASMCs as detected by western blotting (P<0.01). These findings suggest that miR‑31 is able to promote the proliferation and migration of HASMCs, at least in part, by targeting MFN2. The results of the present study provide novel insight into the underlying mechanisms and roles of miR‑31/MFN2 in the pathology of ASO, which may offer a potential therapeutic target for the treatment of ASO.
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Copy and paste a formatted citation
Spandidos Publications style
Huang S, Chen Z, Wu W, Wang M, Wang R, Cui J, Li W and Wang S: MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie. Exp Ther Med 15: 633-640, 2018.
APA
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J. ... Wang, S. (2018). MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie. Experimental and Therapeutic Medicine, 15, 633-640. https://doi.org/10.3892/etm.2017.5453
MLA
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J., Li, W., Wang, S."MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie". Experimental and Therapeutic Medicine 15.1 (2018): 633-640.
Chicago
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J., Li, W., Wang, S."MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie". Experimental and Therapeutic Medicine 15, no. 1 (2018): 633-640. https://doi.org/10.3892/etm.2017.5453
Copy and paste a formatted citation
x
Spandidos Publications style
Huang S, Chen Z, Wu W, Wang M, Wang R, Cui J, Li W and Wang S: MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie. Exp Ther Med 15: 633-640, 2018.
APA
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J. ... Wang, S. (2018). MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie. Experimental and Therapeutic Medicine, 15, 633-640. https://doi.org/10.3892/etm.2017.5453
MLA
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J., Li, W., Wang, S."MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie". Experimental and Therapeutic Medicine 15.1 (2018): 633-640.
Chicago
Huang, S., Chen, Z., Wu, W., Wang, M., Wang, R., Cui, J., Li, W., Wang, S."MicroRNA-31 promotes arterial smooth muscle cell proliferation and migration by targeting mitofusin‑2 in arteriosclerosis obliterans of the lower extremitie". Experimental and Therapeutic Medicine 15, no. 1 (2018): 633-640. https://doi.org/10.3892/etm.2017.5453
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