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Article

Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord

  • Authors:
    • Li Chai
    • Lin Bai
    • Li Li
    • Fei Chen
    • Jie Zhang
  • View Affiliations / Copyright

    Affiliations: Core Facility, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Regenerative Medicine Research Centre, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1076-1080
    |
    Published online on: November 7, 2017
       https://doi.org/10.3892/etm.2017.5456
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Abstract

Stem cell‑based therapy serves a key role in clinical treatments, and mesenchymal stem cells (MSCs) have been widely used in clinical tumor therapy trials. In the present study, MSCs were isolated from umbilical cord (UC) and co‑cultured with the lung cancer cell line H1299. The effects of UC‑derived MSCs (UCMSCs) on H1299 cell invasion and proliferation were evaluated using a Matrigel‑based Transwell assay and CCK8 assay, respectively. Apoptosis and cell cycle progression among H1299 cells were detected by flow cytometry, and kinase expression in H1299 cells was detected by western blotting. The results indicated that UCMSCs significantly inhibited H1299 cell invasion and significantly induced apoptosis of H1299 cells, but exhibited no effect on H1299 cell proliferation and cell cycle progression. It was also identified that H1299 cell expression of key kinases (AKT, phosphoinositide 3‑kinase, signal transducer and activator of transcription 3 and mechanistic target of rapamycin) was significantly suppressed in the presence of UCMSCs. To the best of our knowledge, the present study demonstrates for the first time that UCMSCs have an anti‑tumor effect against lung cancer cells, which may indicate that AKT/phosphoinositide 3‑kinase/signal transducer and activator of transcription 3 signaling is important in the UCMSC‑mediated regulation of H1299 cell functions.
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Copy and paste a formatted citation
Spandidos Publications style
Chai L, Bai L, Li L, Chen F and Zhang J: Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord. Exp Ther Med 15: 1076-1080, 2018.
APA
Chai, L., Bai, L., Li, L., Chen, F., & Zhang, J. (2018). Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord. Experimental and Therapeutic Medicine, 15, 1076-1080. https://doi.org/10.3892/etm.2017.5456
MLA
Chai, L., Bai, L., Li, L., Chen, F., Zhang, J."Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord". Experimental and Therapeutic Medicine 15.1 (2018): 1076-1080.
Chicago
Chai, L., Bai, L., Li, L., Chen, F., Zhang, J."Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1076-1080. https://doi.org/10.3892/etm.2017.5456
Copy and paste a formatted citation
x
Spandidos Publications style
Chai L, Bai L, Li L, Chen F and Zhang J: Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord. Exp Ther Med 15: 1076-1080, 2018.
APA
Chai, L., Bai, L., Li, L., Chen, F., & Zhang, J. (2018). Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord. Experimental and Therapeutic Medicine, 15, 1076-1080. https://doi.org/10.3892/etm.2017.5456
MLA
Chai, L., Bai, L., Li, L., Chen, F., Zhang, J."Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord". Experimental and Therapeutic Medicine 15.1 (2018): 1076-1080.
Chicago
Chai, L., Bai, L., Li, L., Chen, F., Zhang, J."Biological functions of lung cancer cells are suppressed in co‑culture with mesenchymal stem cells isolated from umbilical cord". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1076-1080. https://doi.org/10.3892/etm.2017.5456
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