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Article

Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling

  • Authors:
    • Yan Jiang
    • Wei Wang
    • Zong‑Yang Liu
    • Yi Xie
    • Yuan Qian
    • Xue‑Ni Cai
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The Cancer Hospital of Guizhou, Guiyang, Guizhou 550003, P.R. China, Department of Nephrology, The 455 Hospital of Chinese PLA, Nephrology Center of Nanjing Military Area Command of Chinese PLA, Shanghai 200052, P.R. China
  • Pages: 1021-1028
    |
    Published online on: November 8, 2017
       https://doi.org/10.3892/etm.2017.5465
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Abstract

Tumor necrosis factor (TNF)‑α has been reported to be important in glomerulonephritis, which is closely associated with podocyte dysfunction and apoptosis. However, the precise mechanisms by which TNF‑α expression are regulated remain unclear. The purpose of the present study was to investigate the role of microRNA (miR)‑130a‑3p/301a‑3p in the post‑transcriptional control of TNF‑α expression and high glucose (HG)‑induced podocyte dysfunction. Mice MPC5 podocytes were incubated with HG and transfected with miR‑130a‑3p/301a‑3p mimics or inhibitors, reactive oxygen species (ROS) levels were measured by flow cytometry assay, and the mRNA and protein levels were assayed by using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. The targeted genes were predicted by a bioinformatics algorithm and verified using a dual luciferase reporter assay. It was observed that miR‑130a‑3p/301a‑3p was a novel regulator of TNF‑α in mouse podocytes. miR‑130a‑3p/301a‑3p mimics inhibited TNF‑α 3'‑untranslated region luciferase reporter activity, in addition to endogenous TNF‑α protein expression. Furthermore, forced expression of miR‑130a‑3p or miR‑301a‑3p resulted in the downregulation of ROS and malondialdehyde (MDA) and the upregulation of superoxide dismutase (SOD) 1 in the presence of HG. Inhibition of TNF‑α level prevented a remarkable reduc­tion in SOD activity and a marked increase in ROS and MDA levels in HG‑treated podocytes. Furthermore, TNF‑α loss‑of‑function significantly reversed HG‑induced podocyte apoptosis. These data demonstrated a novel up‑stream role for miR‑130a‑3p/301a‑3p in TNF‑α‑mediated podocyte dysfunction and apoptosis in the presence of HG.
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Copy and paste a formatted citation
Spandidos Publications style
Jiang Y, Wang W, Liu ZY, Xie Y, Qian Y and Cai XN: Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling. Exp Ther Med 15: 1021-1028, 2018.
APA
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., & Cai, X. (2018). Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling. Experimental and Therapeutic Medicine, 15, 1021-1028. https://doi.org/10.3892/etm.2017.5465
MLA
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., Cai, X."Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling". Experimental and Therapeutic Medicine 15.1 (2018): 1021-1028.
Chicago
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., Cai, X."Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1021-1028. https://doi.org/10.3892/etm.2017.5465
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Wang W, Liu ZY, Xie Y, Qian Y and Cai XN: Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling. Exp Ther Med 15: 1021-1028, 2018.
APA
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., & Cai, X. (2018). Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling. Experimental and Therapeutic Medicine, 15, 1021-1028. https://doi.org/10.3892/etm.2017.5465
MLA
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., Cai, X."Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling". Experimental and Therapeutic Medicine 15.1 (2018): 1021-1028.
Chicago
Jiang, Y., Wang, W., Liu, Z., Xie, Y., Qian, Y., Cai, X."Overexpression of miR‑130a‑3p/301a‑3p attenuates high glucose‑induced MPC5 podocyte dysfunction through suppression of TNF‑α signaling". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1021-1028. https://doi.org/10.3892/etm.2017.5465
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