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Article

Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway

  • Authors:
    • Dan Gao
    • Sanhui Jing
    • Qian Zhang
    • Ge Wu
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1029-1035
    |
    Published online on: November 10, 2017
       https://doi.org/10.3892/etm.2017.5479
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Abstract

Previous studies have demonstrated that pterostilbene (Pter) prevents oxidative stress, suppresses cell growth and exhibits anti‑fungal and anti‑inflammatory effects. Pter is used to treat a number of clinical diseases, including Alzheimer's disease, various malignancies and hypercholesteremia. The aim of the present study was to investigate whether Pter protects against acute renal ischemia reperfusion injury (IRI) and inhibits oxidative stress, inducible nitric oxide synthase (iNOS) expression and inflammation in rats. A total of 40 adult male Sprague Dawley rats were divided into the following 5 groups at random: Control group, where rats were not subjected to renal IRI; IRI group, where rats were subjected to renal IRI; Pter 10 group, where rats underwent renal IRI and were treated with 10 mg/kg Pter; Pter 20 group, where rats underwent renal IRI and were treated with 20 mg/kg Pter; Pter 30 group, where rats underwent renal IRI and were treated with 30 mg/kg Pter. The results demonstrated that Pter treatment improved renal function following acute renal IRI. Compared with the untreated renal IRI group, myeloperoxidase, iNOS, interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α expression levels were significantly decreased (P<0.01), whereas IL‑10 expression levels were significantly increased (P<0.01) following treatment with Pter in acute renal IRI rats. In addition, Pter significantly attenuated caspase‑3 activity and the Toll‑like receptor 4 (TLR4)/nuclear factor (NF)‑κB signaling pathway induced by acute renal IRI (P<0.01). These results provide evidence to suggest that administration of Pter may protect against acute renal IRI and inhibit oxidative stress, iNOS expression and inflammation in rats via the TLR4/NF‑κB signaling pathway.
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View References

1 

Zhang N, Cheng GY, Liu XZ and Zhang FJ: Expression of Bcl-2 and NF-κB in brain tissue after acute renal ischemia-reperfusion in rats. Asian Pac J Trop Med. 7:386–389. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Novak KB, Le HD, Christison-Lagay ER, Nose V, Doiron RJ, Moses MA and Puder M: Effects of metalloproteinase inhibition in a murine model of renal ischemia-reperfusion injury. Pediatr Res. 67:257–262. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Ogawa T and Mimura Y: Antioxidant effect of zinc on acute renal failure induced by ischemia-reperfusion injury in rats. Am J Nephrol. 19:609–614. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Wu H, Steenstra R, de Boer EC, Zhao CY, Ma J, van der Stelt JM and Chadban SJ: Preconditioning with recombinant high-mobility group box 1 protein protects the kidney against ischemia-reperfusion injury in mice. Kidney Int. 85:824–832. 2014. View Article : Google Scholar : PubMed/NCBI

5 

He W, Zhang Y, Zhang J, Yu Q, Wang P, Wang Z and Smith AJ: Cytidine-phosphate-guanosine oligonucleotides induce interleukin-8 production through activation of TLR9, MyD88, NF-κB, and ERK pathways in odontoblast cells. J Endod. 38:780–785. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Andreucci M, Faga T, Lucisano G, Uccello F, Pisani A, Memoli B, Sabbatini M, Fuiano G and Michael A: Mycophenolic acid inhibits the phosphorylation of NF-kappaB and JNKs and causes a decrease in IL-8 release in H2O2-treated human renal proximal tubular cells. Chem Biol Interact. 185:253–262. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Li Y, Qiang X, Li Y, Yang X, Luo L, Xiao G, Cao Z, Tan Z and Deng Y: Pterostilbene-O-acetamidoalkylbenzylamines derivatives as novel dual inhibitors of cholinesterase with anti-β-amyloid aggregation and antioxidant properties for the treatment of Alzheimer's disease. Bioorg Med Chem Lett. 26:2035–2039. 2016. View Article : Google Scholar : PubMed/NCBI

8 

McCormack D and McFadden D: A review of pterostilbene antioxidant activity and disease modification. Oxid Med Cell Longev. 2013:5754822013. View Article : Google Scholar : PubMed/NCBI

9 

McCormack D and McFadden D: Pterostilbene and cancer: Current review. J Surg Res. 173:e53–e61. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Chang J, Rimando A, Pallas M, Camins A, Porquet D, Reeves J, Shukitt-Hale B, Smith MA, Joseph JA and Casadesus G: Low-dose pterostilbene, but not resveratrol, is a potent neuromodulator in aging and Alzheimer's disease. Neurobiol Aging. 33:2062–2071. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Pari L and Satheesh MA: Effect of pterostilbene on hepatic key enzymes of glucose metabolism in streptozotocin- and nicotinamide-induced diabetic rats. Life Sci. 79:641–645. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Li YY, Zhai W, Yang X, Ding J and Kan L: Effects of Panax notoginseng saponins on proliferation, apoptosis and cell cycle of K562 cells in vitro and the mechanisms. Nan Fang Yi Ke Da Xue Xue Bao. 35:1103–1109. 2015.(In Chinese). PubMed/NCBI

13 

Hilmi IA, Peng Z, Planinsic RM, Damian D, Dai F, Tyurina YY, Kagan VE and Kellum JA: N-acetylcysteine does not prevent hepatorenal ischaemia-reperfusion injury in patients undergoing orthotopic liver transplantation. Nephrol Dial Transplant. 25:2328–2333. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Freitas MC, Uchida Y, Lassman C, Danovitch GM, Busuttil RW and Kupiec-Weglinski JW: Type I interferon pathway mediates renal ischemia/reperfusion injury. Transplantation. 92:131–138. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Asaga T, Ueki M, Chujo K and Taie S: JTE-607, an inflammatory cytokine synthesis inhibitor, attenuatesischemia/reperfusion-induced renal injury by reducing neutrophil activation in rats. J Biosci Bioeng. 106:22–26. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Lafci G, Gedik HS, Korkmaz K, Erdem H, Cicek OF, Nacar OA, Yildirim L, Kaya E and Ankarali H: Efficacy of iloprost and montelukast combination on spinal cord ischemia/reperfusion injury in a rat model. J Cardiothorac Surg. 8:642013. View Article : Google Scholar : PubMed/NCBI

17 

Pan MH, Chang YH, Tsai ML, Lai CS, Ho SY, Badmaev V and Ho CT: Pterostilbene suppressed lipopolysaccharide-induced up-expression of iNOS and COX-2 in murine macrophages. J Agric Food Chem. 56:7502–7509. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wan X, Huang WJ, Chen W, Xie HG, Wei P, Chen X and Cao CC: IL-10 deficiency increases renal ischemia-reperfusion injury. Nephron Exp Nephrol. 128:37–45. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Zhang F, Lu M, Wang H and Ren T: Aspirin attenuates angiotensin II-induced inflammation in bone marrow mesenchymal stem cells via the inhibition of ERK1/2 and NF-κB activation. Biomed Rep. 1:930–934. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Tsushima F, Tanaka K, Otsuki N, Youngnak P, Iwai H, Omura K and Azuma M: Predominant expression of B7-H1 and its immunoregulatory roles in oral squamous cell carcinoma. Oral Oncol. 42:268–274. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Jung M, Sola A, Hughes J, Kluth DC, Vinuesa E, Viñas JL, Pérez-Ladaga A and Hotter G: Infusion of IL-10-expressing cells protects against renal ischemia through induction of lipocalin-2. Kidney Int. 81:969–982. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Tsai ML, Lai CS, Chang YH, Chen WJ, Ho CT and Pan MH: Pterostilbene, a natural analogue of resveratrol, potently inhibits 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse skin carcinogenesis. Food Funct. 3:1185–1194. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Wang BC and Ma J: Role of microRNAs in malignant glioma. Chin Med J (Engl). 128:1238–1244. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Wang C, Sun H, Song Y, Ma Z, Zhang G, Gu X and Zhao L: Pterostilbene attenuates inflammation in rat heart subjected to ischemia-reperfusion: Role of TLR4/NF-κB signaling pathway. Int J Clin Exp Med. 8:1737–1746. 2015.PubMed/NCBI

25 

Zhang J, Xia J, Zhang Y, Xiao F, Wang J, Gao H, Liu Y, Rong S, Yao Y, Xu G and Li J: HMGB1-TLR4 signaling participates in renal ischemia reperfusion injury and could be attenuated by dexamethasone-mediated inhibition of the ERK/NF-κB pathway. Am J Transl Res. 8:4054–4067. 2016.PubMed/NCBI

26 

O'Neill S, Humphries D, Tse G, Marson LP, Dhaliwal K, Hughes J, Ross JA, Wigmore SJ and Harrison EM: Heat shock protein 90 inhibition abrogates TLR4-mediated NF-κB activity and reduces renal ischemia-reperfusion injury. Sci Rep. 5:129582015. View Article : Google Scholar : PubMed/NCBI

27 

Cichocki M, Paluszczak J, Szaefer H, Piechowiak A, Rimando AM and Baer-Dubowska W: Pterostilbene is equally potent as resveratrol in inhibiting 12-O-tetradecanoylphorbol-13-acetate activated NFkappaB, AP-1, COX-2, and iNOS in mouse epidermis. Mol Nutr Food Res. 52 Suppl 1:S62–S70. 2008.PubMed/NCBI

28 

Mankan AK, Lawless MW, Gray SG, Kelleher D and McManus R: NF-kappaB regulation: The nuclear response. J Cell Mol Med. 13:631–643. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Liang D, Sun Y, Shen Y, Li F, Song X, Zhou E, Zhao F, Liu Z, Fu Y, Guo M, et al: Shikonin exerts anti-inflammatory effects in a murine model of lipopolysaccharide-induced acute lung injury by inhibiting the nuclear factor-kappaB signaling pathway. Int Immunopharmacol. 16:475–480. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Xu D, Niu W, Luo Y, Zhang B, Liu M, Dong H, Liu Y and Li Z: Endogenous estrogen attenuates hypoxia-induced pulmonary hypertension by inhibiting pulmonary arterial vasoconstriction and pulmonary arterial smooth muscle cells proliferation. Int J Med Sci. 10:771–781. 2013. View Article : Google Scholar : PubMed/NCBI

31 

D'Hooghe TM and Debrock S: Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons. Hum Reprod Update. 8:84–88. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Gao D, Jing S, Zhang Q and Wu G: Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway. Exp Ther Med 15: 1029-1035, 2018.
APA
Gao, D., Jing, S., Zhang, Q., & Wu, G. (2018). Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway. Experimental and Therapeutic Medicine, 15, 1029-1035. https://doi.org/10.3892/etm.2017.5479
MLA
Gao, D., Jing, S., Zhang, Q., Wu, G."Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway". Experimental and Therapeutic Medicine 15.1 (2018): 1029-1035.
Chicago
Gao, D., Jing, S., Zhang, Q., Wu, G."Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1029-1035. https://doi.org/10.3892/etm.2017.5479
Copy and paste a formatted citation
x
Spandidos Publications style
Gao D, Jing S, Zhang Q and Wu G: Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway. Exp Ther Med 15: 1029-1035, 2018.
APA
Gao, D., Jing, S., Zhang, Q., & Wu, G. (2018). Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway. Experimental and Therapeutic Medicine, 15, 1029-1035. https://doi.org/10.3892/etm.2017.5479
MLA
Gao, D., Jing, S., Zhang, Q., Wu, G."Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway". Experimental and Therapeutic Medicine 15.1 (2018): 1029-1035.
Chicago
Gao, D., Jing, S., Zhang, Q., Wu, G."Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll‑like receptor 4/nuclear factor‑κB signaling pathway". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1029-1035. https://doi.org/10.3892/etm.2017.5479
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