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Article

TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells

  • Authors:
    • Jihua Pei
    • Na Song
    • Limin Wu
    • Jinbo Qi
    • Shenglong Xia
    • Changlong Xu
    • Bo Zheng
    • Jun Yang
    • Yanyan Qiu
    • Haijun Wang
    • Yi Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Molecular Biology and Biochemistry, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Pathology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
  • Pages: 1041-1047
    |
    Published online on: November 13, 2017
       https://doi.org/10.3892/etm.2017.5493
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Abstract

Fibroblast growth factor 21 (FGF21) as a member of the FGFs serves a key role in glucose homeostasis and protection of the liver, heart, kidney and skin from damage as well as cancer cell development. In addition, transcription of FGF21 is sensitive to diverse damages; however, the role of the transcriptional regulator of FGF21 in cancer cells remains to be elucidated. FGFs were identified to have dominant expression in cancer cells; therefore, mouse forestomach carcinoma (MFC) cells were used in the present study, which is a mouse stomach cancer cell strain for identifying the FGF21 regulators. In promoter analysis of FGF21, the putative transcription factor 4 (TCF4) binding motifs (T/AC/GAAAG) were observed within 1.5 kb of the promoter region. Further chromatin immunoprecipitation and yeast‑one hybrid assays identified that TCF4 directly bound to one of the two putative binding motifs observed. A co‑immunoprecipitation assay identified that β‑catenin interacts with TCF4 in MFC cells, and the β‑catenin/TCF4 complex bound to the promoter of FGF21. In order to examine the function of TCF4 and β‑catenin in transcriptional regulation of FGF21, TCF4 and β‑catenin was transiently expressed in MFC cells. Reverse transcription‑quantitative polymerase chain reaction results revealed that overexpression of TCF4 and β‑catenin activated FGF21 transcription. Besides, suppression of β‑catenin via a specific short interfering RNA resulted in reduction of FGF21 expression. Together these findings suggest that the β‑catenin/TCF complex directly activates FGF21 via promoter binding. The observations of the present study may help elucidate the regulatory mechanism of FGF21, which is a key pharmaceutical protein.
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Copy and paste a formatted citation
Spandidos Publications style
Pei J, Song N, Wu L, Qi J, Xia S, Xu C, Zheng B, Yang J, Qiu Y, Wang H, Wang H, et al: TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells. Exp Ther Med 15: 1041-1047, 2018.
APA
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C. ... Jiang, Y. (2018). TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells. Experimental and Therapeutic Medicine, 15, 1041-1047. https://doi.org/10.3892/etm.2017.5493
MLA
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C., Zheng, B., Yang, J., Qiu, Y., Wang, H., Jiang, Y."TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells". Experimental and Therapeutic Medicine 15.1 (2018): 1041-1047.
Chicago
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C., Zheng, B., Yang, J., Qiu, Y., Wang, H., Jiang, Y."TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1041-1047. https://doi.org/10.3892/etm.2017.5493
Copy and paste a formatted citation
x
Spandidos Publications style
Pei J, Song N, Wu L, Qi J, Xia S, Xu C, Zheng B, Yang J, Qiu Y, Wang H, Wang H, et al: TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells. Exp Ther Med 15: 1041-1047, 2018.
APA
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C. ... Jiang, Y. (2018). TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells. Experimental and Therapeutic Medicine, 15, 1041-1047. https://doi.org/10.3892/etm.2017.5493
MLA
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C., Zheng, B., Yang, J., Qiu, Y., Wang, H., Jiang, Y."TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells". Experimental and Therapeutic Medicine 15.1 (2018): 1041-1047.
Chicago
Pei, J., Song, N., Wu, L., Qi, J., Xia, S., Xu, C., Zheng, B., Yang, J., Qiu, Y., Wang, H., Jiang, Y."TCF4/β‑catenin complex is directly upstream of FGF21 in mouse stomach cancer cells". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1041-1047. https://doi.org/10.3892/etm.2017.5493
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