(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1

  • Authors:
    • Sufang Liu
    • Hongde Li
    • Min Tang
    • Ya Cao
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/etm.2017.5495
  • Pages: 1105-1112
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Abstract

The Epstein‑Barr virus (EBV) lytic cycle contributes to the development of EBV‑associated diseases. EBV‑encoded latent membrane protein 1 (LMP1) is key to EBV lytic replication, and our previous work indicated that epigallocatechin‑3‑gallate (EGCG) inhibited constitutive EBV lytic infection through the suppression of LMP1‑activated phosphoinositide 3‑kinase/Akt and mitogen‑activated protein kinase kinase/extracellular signal‑related protein kinase 1/2 signaling. The present study demonstrated that LMP1 in CNE‑LMP1 constructed cells significantly induced the expression of the EBV lytic proteins BZLF1 (P<0.001) and BMRF1 (P<0.05) compared with CNE1 cells. Following treatment with a specific DNAzyme that targets LMP1, significantly reduced protein expression levels of BZLF1 and BMRF1 in EBV‑associated epithelial carcinoma CNE1‑LMP1 cells (P<0.001 and P<0.01, respectively) and lymphoma B95.8 cells (both P<0.01) were observed. Furthermore, EGCG significantly inhibited the mRNA and protein expression levels of LMP1 (P<0.05) in an apparent dose‑dependent manner in CNE1‑LMP1 and B95.8 cells. Thus, the present findings indicated that the molecular mechanism underlying EGCG inhibition of EBV lytic infection involves downregulation of LMP1.
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January-2018
Volume 15 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Liu S, Li H, Tang M and Cao Y: (-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1. Exp Ther Med 15: 1105-1112, 2018
APA
Liu, S., Li, H., Tang, M., & Cao, Y. (2018). (-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1. Experimental and Therapeutic Medicine, 15, 1105-1112. https://doi.org/10.3892/etm.2017.5495
MLA
Liu, S., Li, H., Tang, M., Cao, Y."(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1". Experimental and Therapeutic Medicine 15.1 (2018): 1105-1112.
Chicago
Liu, S., Li, H., Tang, M., Cao, Y."(-)-Epigallocatechin‑3‑gallate inhibition of Epstein‑Barr virus spontaneous lytic infection involves downregulation of latent membrane protein 1". Experimental and Therapeutic Medicine 15, no. 1 (2018): 1105-1112. https://doi.org/10.3892/etm.2017.5495