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Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord

  • Authors:
    • Hao Zhang
    • Yanling Tao
    • Saisai Ren
    • Haihui Liu
    • Hui Zhou
    • Jiangwei Hu
    • Yongyong Tang
    • Bin Zhang
    • Hu Chen
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, Affiliated Hospital of Jining Medical University, Jining, Shandong 272000, P.R. China, Graduate Department, School of Medicine, Jining Medical University, Jining, Shandong 272000, P.R. China, Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital to Academy of Military Medical Sciences, Beijing 100071, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 806-812
    |
    Published online on: November 13, 2017
       https://doi.org/10.3892/etm.2017.5502
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Abstract

The human umbilical cord (UC) is usually discarded as biological waste. However, it has attracted interest as a source of cells including endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs), which have demonstrated enormous potential in regenerative medicine. The present study describes a convenient protocol that has been developed to sequentially extract these two cell types from a single UC. EPCs which had properties of progenitor cells were successfully isolated from the UC vein. These cells had cobble‑shaped morphology and expressed Flt‑1, KDR, VE‑cadherin, von Willebrand factor and CD31 mRNA, in addition to CD73, CD105 and vascular endothelial growth factor receptor‑2. In addition to absorbing fluorescent‑labeled acetylated low density protein and binding to fluorescein isothiocyanate‑UEA‑l, they were able to form vascular tube‑like structures on Matrigel. Typical fibroblast‑like cells, which were isolated from the Wharton's jelly, were confirmed to be MSCs by their expression of CD73, CD90 and CD105, and their ability to differentiate into adipocytes and osteoblasts. Thus, the human UC‑derived cells may be suitable for use in tissue engineering and cell therapy.
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1 

Timmermans F, Plum J, Yöder MC, Ingram DA, Vandekerckhove B and Case J: Endothelial progenitor cells: Identity defined? J Cell Mol Med. 13:87–102. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Kang SD, Carlon TA, Jantzen AE, Lin FH, Ley MM, Allen JD, Stabler TV, Haley NR, Truskey GA and Achneck HE: Isolation of functional human endothelial cells from small volumes of umbilical cord blood. Ann Biomed Eng. 41:2181–2192. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Kalka C, Masuda H, Takahashi T, Kalka-Moll WM, Silver M, Kearney M, Li T, Isner JM and Asahara T: Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci USA. 97:pp. 3422–3427. 2000; View Article : Google Scholar : PubMed/NCBI

4 

Fan CL, Li Y, Gao PJ, Liu JJ, Zhang XJ and Zhu DL: Differentiation of endothelial progenitor cells from human umbilical cord blood CD 34+ cells in vitro. Acta Pharmacol Sin. 24:212–218. 2003.PubMed/NCBI

5 

Rafii S and Lyden D: Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med. 9:702–712. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S and Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 284:143–147. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Toma C, Pittenger MF, Cahill KS, Byrne BJ and Kessler PD: Human mesenchymal stem cells differentiate to a cardiomyocyte phenotype in the adult murine heart. Circulation. 105:93–98. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Secco M, Zucconi E, Vieira NM, Fogaça LL, Cerqueira A, Carvalho MD, Jazedje T, Okamoto OK, Muotri AR and Zatz M: Multipotent stem cells from umbilical cord: Cord is richer than blood! Stem Cells. 26:1–150. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Bongso A, Fong CY and Gauthaman K: Taking stem cells to the clinic: Major challenges. J Cell Biochem. 105:1352–1360. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Hayward CJ, Fradette J, Galbraith T, Rémy M, Guignard R, Gauvin R, Germain L and Auger FA: Harvesting the potential of the human umbilical cord: Isolation and characterisation of four cell types for tissue engineering applications. Cells Tissues Organs. 197:37–54. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Wang SH, Lin SJ, Chen YH, Lin FY, Shih JC, Wu CC, Wu HL and Chen YL: Late outgrowth endothelial cells derived from Wharton jelly in human umbilical cord reduce neointimal formation after vascular injury: Involvement of pigment epithelium-derived factor. Arterioscler Thromb Vasc Biol. 29:816–822. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Chao KC, Chao KF, Fu YS and Liu SH: Islet-like clusters derived from mesenchymal stem cells in Wharton's Jelly of the human umbilical cord for transplantation to control type 1 diabetes. PLoS One. 3:e14512008. View Article : Google Scholar : PubMed/NCBI

13 

Zhang H, Zhang B, Tao Y, Cheng M, Hu J, Xu M and Chen H: Isolation and characterization of mesenchymal stem cells from whole human umbilical cord applying a single enzyme approach. Cell Biochem Funct. 30:643–649. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC and Chen CC: Mesenchymal stem cells in the Wharton's jelly of the human umbilical cord. Stem Cells. 22:1330–1337. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Sarugaser R, Lickorish D, Baksh D, Hosseini MM and Davies JE: Human umbilical cord perivascular (HUCPV) cells: A source of mesenchymal progenitors. Stem Cells. 23:220–229. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Wang J, Kimura T, Asada R, Harada S, Yokota S, Kawamoto Y, Fujimura Y, Tsuji T, Ikehara S and Sonoda Y: SCID-repopulating cell activity of human cord blood-derived CD34-cells assured by intra-bone marrow injection. Blood. 101:2924–2931. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Yahata T, Ando K, Sato T, Miyatake H, Nakamura Y, Muguruma Y, Kato S and Hotta T: A highly sensitive strategy for SCID-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow. Blood. 101:2905–2913. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Hur J, Yoon CH, Kim HS, Choi JH, Kang HJ, Hwang KK, Oh BH, Lee MM and Park YB: Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 24:288–293. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Murga M, Yao L and Tosato G: Derivation of endothelial cells from CD34-umbilical cord blood. Stem Cells. 22:385–395. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Moon SH, Kim SM, Park SJ, Kim H, Bae D, Choi YS and Chung HM: Development of a xeno-free autologous culture system for endothelial progenitor cells derived from human umbilical cord blood. PLoS One. 8:e752242013. View Article : Google Scholar : PubMed/NCBI

21 

Huang ZW, Liu N, Li D, Zhang HY, Wang Y, Liu Y, Zhang LL and Ju XL: Angiopoietin-1 modified human umbilical cord mesenchymal stem cell therapy for endotoxin-induced acute lung injury in rats. Yonsei Med J. 58:206–216. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Lee MJ, Yoon TG, Kang M, Kim HJ and Kang KS: Effect of subcutaneous treatment with human umbilical cord blood-derived multipotent stem cells on peripheral neuropathic pain in rats. Korean J Physiol Pharmacol. 21:153–160. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Kumbhar SG and Pawar SH: Synthesis and characterization of chitosan-alginate scaffolds for seeding human umbilical cord derived mesenchymal stem cells. Biomed Mater Eng. 27:561–575. 2016.PubMed/NCBI

24 

Marupanthorn K, Tantrawatpan C, Kheolamai P, Tantikanlayaporn D and Manochantr S: Bone morphogenetic protein-2 enhances the osteogenic differentiation capacity of mesenchymal stromal cells derived from human bone marrow and umbilical cord. Int J Mol Med. 39:654–662. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Yin C, Liang Y, Zhang J, Ruan G, Li Z, Pang R and Pan X: Umbilical cord-derived mesenchymal stem cells relieve hindlimb ischemia through enhancing Angiogenesis in tree shrews. Stem Cells Int. 2016:97420342016. View Article : Google Scholar : PubMed/NCBI

26 

Zayed SA, Gaafar TM, Samy RM, Sabry D, Nasr AS and Maksoud FA: Production of endothelial progenitor cells obtained from human Wharton's jelly using different culture conditions. Biotech Histochem. 91:532–539. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Meng X, Xue M, Xu P, Hu F, Sun B and Xiao Z: MicroRNA profiling analysis revealed different cellular senescence mechanisms in human mesenchymal stem cells derived from different origin. Genomics. 109:147–157. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Lo Iacono M, Anzalone R, La Rocca G, Baiamonte E, Maggio A and Acuto S: Wharton's jelly mesenchymal stromal cells as a feeder layer for the ex vivo expansion of hematopoietic stem and progenitor cells: A review. Stem Cell Rev. 13:35–49. 2017. View Article : Google Scholar : PubMed/NCBI

29 

Fellows CR, Matta C, Zakany R, Khan IM and Mobasheri A: Adipose, bone marrow and synovial joint-derived mesenchymal stem cells for cartilage repair. Front Genet. 7:2132016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang H, Tao Y, Ren S, Liu H, Zhou H, Hu J, Tang Y, Zhang B and Chen H: Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord. Exp Ther Med 15: 806-812, 2018.
APA
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J. ... Chen, H. (2018). Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord. Experimental and Therapeutic Medicine, 15, 806-812. https://doi.org/10.3892/etm.2017.5502
MLA
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J., Tang, Y., Zhang, B., Chen, H."Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord". Experimental and Therapeutic Medicine 15.1 (2018): 806-812.
Chicago
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J., Tang, Y., Zhang, B., Chen, H."Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord". Experimental and Therapeutic Medicine 15, no. 1 (2018): 806-812. https://doi.org/10.3892/etm.2017.5502
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Tao Y, Ren S, Liu H, Zhou H, Hu J, Tang Y, Zhang B and Chen H: Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord. Exp Ther Med 15: 806-812, 2018.
APA
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J. ... Chen, H. (2018). Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord. Experimental and Therapeutic Medicine, 15, 806-812. https://doi.org/10.3892/etm.2017.5502
MLA
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J., Tang, Y., Zhang, B., Chen, H."Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord". Experimental and Therapeutic Medicine 15.1 (2018): 806-812.
Chicago
Zhang, H., Tao, Y., Ren, S., Liu, H., Zhou, H., Hu, J., Tang, Y., Zhang, B., Chen, H."Simultaneous harvesting of endothelial progenitor cells and mesenchymal stem cells from the human umbilical cord". Experimental and Therapeutic Medicine 15, no. 1 (2018): 806-812. https://doi.org/10.3892/etm.2017.5502
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