Protective effect of histatin 1 against ultraviolet‑induced damage to human corneal epithelial cells

Huang,Guo‑Qiang; Yi,Guo‑Guo; Wu,Lai‑Wei; Feng,Song‑Fu; Wu,Wei; Peng,Ling; Yi,Rui‑Wen; Ma,Wenbei; Lu,Xiaohe

doi:10.3892/etm.2017.5503

January-2018 Volume 15 Issue 1

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January-2018 Volume 15 Issue 1

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Article Open Access

Protective effect of histatin 1 against ultraviolet‑induced damage to human corneal epithelial cells

Authors:
- Guo‑Qiang Huang
- Guo‑Guo Yi
- Lai‑Wei Wu
- Song‑Fu Feng
- Wei Wu
- Ling Peng
- Rui‑Wen Yi
- Wenbei Ma
- Xiaohe Lu
View Affiliations / Copyright

Affiliations: Department of Ophthalmology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China

Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 679-684
|
Published online on: November 13, 2017

https://doi.org/10.3892/etm.2017.5503
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Abstract

The aim of the present study was to investigate the role of histatin 1 (Hst1) in human corneal epithelial cells (HCECs) exposed to ultraviolet (UV) radiation. Prior to UV irradiation for various durations, HCECs were pre‑treated with different concentrations of Hst1 and the effect on cell apoptosis and cell viability were examined by flow cytometry, alamarBlue® and MTT assays to determine the optimal concentration of Hst1 and UV dose. Cells were then subjected to quantitative PCR, ELISA and western blot analysis to determine the expression of cell damage‑associated genes. HCECs exposed to UV light for 1 h displayed decreased viability when compared to that of control cells, and a 3 h UV exposure markedly increased the apoptotic rate of HECEs, while apoptosis was inhibited by pre‑treatment with Hst1. UV radiation downregulated expression of insulin‑like growth factor (IGF)‑1 and B‑cell lymphoma 2 (Bcl‑2), while it upregulated Bcl‑2‑associated X protein (Bax) expression. Hst1 protected HCECs against UV‑induced damage by upregulating the expression of IGF‑1 protein and increasing the Bcl‑2/Bax ratio. In conclusion, Hst1 may prevent UV‑induced damage to corneal epithelial tissue injury and promote its healing.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Protective effect of histatin 1 against ultraviolet‑induced damage to human corneal epithelial cells

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