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Article

Roles of microRNA-539 and osteopontin in rheumatoid arthritis

  • Authors:
    • Yan Liu
    • Kai Qian
    • Chunmei Li
    • Yi Ma
    • Xingguo Chen
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, Jiangsu 223300, P.R. China, Department of Rheumatology, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China
  • Pages: 2681-2687
    |
    Published online on: December 21, 2017
       https://doi.org/10.3892/etm.2017.5665
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Abstract

The present study aimed to investigate the role and mechanism of microRNA-539 (miR-539) in rheumatoid arthritis (RA). A total of 68 RA patients and 46 osteoarthritis patients were enrolled into the current study. Peripheral blood and joint fluid were collected prior to treatment. Reverse transcription‑quantitative polymerase chain reaction was performed to detect osteopontin (OPN) mRNA and miR‑539 expression levels, while ELISA and western blot analysis were applied to detect OPN protein expression. In addition, bioinformatics analysis predicted that miR‑539 directly targeted OPN, while dual‑luciferase assay was used to validate this finding. Furthermore, agomiR‑539 transfection and OPN knockdown by siRNA were conducted in MH7A cells, and MTT assay was used to detect MH7A cell proliferation. The results indicated that OPN was significantly increased in the blood and joint fluid of RA patients, while miR‑539 expression was significantly decreased in the two types of specimens (P<0.05). Subsequent to silencing OPN by siRNA, the proliferation of MH7A cells was decreased (P<0.05). Following upregulation of miR‑539, OPN expression was significantly decreased and cell proliferation was inhibited. Dual‑luciferase assay revealed that miR‑539 regulated OPN expression through complementary binding to 3'‑untranslated region. OPN was also significantly increased in the blood and joint fluid of RA patients, which may be associated with the downregulation of miR‑539. Thus, miR‑539 may promote the development and progression of RA through regulating OPN.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Qian K, Li C, Ma Y and Chen X: Roles of microRNA-539 and osteopontin in rheumatoid arthritis. Exp Ther Med 15: 2681-2687, 2018.
APA
Liu, Y., Qian, K., Li, C., Ma, Y., & Chen, X. (2018). Roles of microRNA-539 and osteopontin in rheumatoid arthritis. Experimental and Therapeutic Medicine, 15, 2681-2687. https://doi.org/10.3892/etm.2017.5665
MLA
Liu, Y., Qian, K., Li, C., Ma, Y., Chen, X."Roles of microRNA-539 and osteopontin in rheumatoid arthritis". Experimental and Therapeutic Medicine 15.3 (2018): 2681-2687.
Chicago
Liu, Y., Qian, K., Li, C., Ma, Y., Chen, X."Roles of microRNA-539 and osteopontin in rheumatoid arthritis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2681-2687. https://doi.org/10.3892/etm.2017.5665
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Qian K, Li C, Ma Y and Chen X: Roles of microRNA-539 and osteopontin in rheumatoid arthritis. Exp Ther Med 15: 2681-2687, 2018.
APA
Liu, Y., Qian, K., Li, C., Ma, Y., & Chen, X. (2018). Roles of microRNA-539 and osteopontin in rheumatoid arthritis. Experimental and Therapeutic Medicine, 15, 2681-2687. https://doi.org/10.3892/etm.2017.5665
MLA
Liu, Y., Qian, K., Li, C., Ma, Y., Chen, X."Roles of microRNA-539 and osteopontin in rheumatoid arthritis". Experimental and Therapeutic Medicine 15.3 (2018): 2681-2687.
Chicago
Liu, Y., Qian, K., Li, C., Ma, Y., Chen, X."Roles of microRNA-539 and osteopontin in rheumatoid arthritis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2681-2687. https://doi.org/10.3892/etm.2017.5665
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