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Article

Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway

  • Authors:
    • Junjian Wang
    • Shaoxiang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Tianjin 5th Central Hospital, Tianjin 300450, P.R. China
  • Pages: 2667-2673
    |
    Published online on: December 21, 2017
       https://doi.org/10.3892/etm.2017.5666
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Abstract

Lung cancer is the most prevalent malignant tumor type in the developed world and the discovery of novel anti‑tumor drugs is a research hotspot. Fisetin, a naturally occurring flavonoid, has been reported to have anti‑cancer effects in multiple tumor types. The present study found that fisetin inhibited the growth and migration of non‑small cell lung cancer in vitro. MTT, wound‑healing, cell‑matrix adhesion and Transwell assays were performed and demonstrated that fisetin suppressed proliferation, migration, adhesion and invasion, respectively. Flow cytometric analysis indicated that fisetin induced apoptosis in the A549 cell line by decreasing the expression of c‑myc, cyclin‑D1, cyclooxygenase‑2, B cell lymphoma-2, CXC chemokine receptor type 4, cluster of differentiation 44 and metalloproteinase‑2/9, increasing the expression of cyclin dependent kinase inhibitor (CDKN) 1A/B, CDKN2D and E‑cadherin and increasing the activity of caspase‑3/9 via targeting the extracellular signal‑regulated kinase signaling pathway. The results provided comprehensive evidence for the anti‑tumor effects of fisetin in non‑small cell lung cancer in vitro, which may provide a novel approach for clinical treatment.
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1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Sullivan I and Planchard D: ALK inhibitors in non-small cell lung cancer: The latest evidence and developments. Ther Adv Med Oncol. 8:32–47. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Luque M, Díez FJ and Disdier C: Optimal sequence of tests for the mediastinal staging of non-small cell lung cancer. BMC Med Inform Decis Mak. 16:92016. View Article : Google Scholar : PubMed/NCBI

4 

Kawasaki K, Sato Y, Suzuki Y, Saito H, Nomura Y and Yoshida Y: Prognostic factors for surgically resected N2 non-small cell lung cancer. Ann Thorac Cardiovasc Surg. 21:217–222. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Hsu YL, Kuo PL and Lin CC: Proliferative inhibition, cell-cycle dysregulation, and induction of apoptosis by ursolic acid in human non-small cell lung cancer A549 cells. Life Sci. 75:2303–2316. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Arai Y, Watanabe S, Kimira M, Shimoi K, Mochizuki R and Kinae N: Dietary intakes of flavonols, flavones and isoflavones by Japanese women and the inverse correlation between quercetin intake and plasma LDL cholesterol concentration. J Nutr. 130:2243–2250. 2000.PubMed/NCBI

7 

Haddad AQ, Venkateswaran V, Viswanathan L, Teahan SJ, Fleshner NE and Klotz LH: Novel antiproliferative flavonoids induce cell cycle arrest in human prostate cancer cell lines. Prostate Cancer Prostatic Dis. 9:68–76. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Suh Y, Afaq F, Khan N, Johnson JJ, Khusro FH and Mukhtar H: Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells. Carcinogenesis. 31:1424–1433. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Pal HC, Sharma S, Elmets CA, Athar M and Afaq F: Fisetin inhibits growth, induces G2/M arrest and apoptosis of human epidermoid carcinoma A431 cells: Role of mitochondrial membrane potential disruption and consequent caspases activation. Exp Dermatol. 22:470–475. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Suh Y, Afaq F, Johnson JJ and Mukhtar H: A plant flavonoid fisetin induces apoptosis in colon cancer cells by inhibition of COX2 and Wnt/EGFR/NF-kappaB-signaling pathways. Carcinogenesis. 30:300–307. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Khan N, Asim M, Afaq F, Abu Zaid M and Mukhtar H: A novel dietary flavonoid fisetin inhibits androgen receptor signaling and tumor growth in athymic nude mice. Cancer Res. 68:8555–8563. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Khan N, Afaq F, Syed DN and Mukhtar H: Fisetin, a novel dietary flavonoid, causes apoptosis and cell cycle arrest in human prostate cancer LNCaP cells. Carcinogenesis. 29:1049–1056. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Chen YC, Shen SC, Lee WR, Lin HY, Ko CH, Shih CM and Yang LL: Wogonin and fisetin induction of apoptosis through activation of caspase 3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1. Arch Toxicol. 76:351–359. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Maher P, Akaishi T and Abe K: Flavonoid fisetin promotes ERK-dependent long-term potentiation and enhances memory. Proc Natl Acad Sci USA. 103:pp. 16568–16573. 2006; View Article : Google Scholar : PubMed/NCBI

15 

Hou DX, Fukuda M, Johnson JA, Miyamori K, Ushikai M and Fujii M: Fisetin induces transcription of NADPH: Quinone oxidoreductase gene through an antioxidant responsive element-involved activation. Int J Oncol. 18:1175–1179. 2001.PubMed/NCBI

16 

Haddad AQ, Venkateswaran V, Viswanathan L, Teahan SJ, Fleshner NE and Klotz LH: Novel antiproliferative flavonoids induce cell cycle arrest in human prostate cancer cell lines. Prostate Cancer Prostatic Dis. 9:68–76. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Chen YC, Shen SC, Lee WR, Lin HY, Ko CH, Shih CM and Yang LL: Wogonin and fisetin induction of apoptosis through activation of caspase 3 cascade and alternative expression of p21 protein in hepatocellular carcinoma cells SK-HEP-1. Arch Toxicol. 76:351–359. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Lu H, Chang DJ, Baratte B, Meijer L and Schulze-Gahmen U: Crystal structure of a human cyclin-dependent kinase 6 complex with a flavonol inhibitor, fisetin. J Med Chem. 48:737–743. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Lee WR, Shen SC, Lin HY, Hou WC, Yang LL and Chen YC: Wogonin and fisetin induce apoptosis in human promyeloleukemic cells, accompanied by a decrease of reactive oxygen species, and activation of caspase 3 and Ca(2+)-dependent endonuclease. Biochem Pharmacol. 63:225–236. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Yao K, Zhang L, Zhang Y, Ye P and Zhu N: The flavonoid, fisetin, inhibits UV radiation-induced oxidative stress and the activation of NF-kappaB and MAPK signaling in human lens epithelial cells. Mol Vis. 14:1865–1871. 2008.PubMed/NCBI

21 

Murtaza I, Adhami VM, Hafeez BB, Saleem M and Mukhtar H: Fisetin, a natural flavonoid, targets chemoresistant human pancreatic cancer AsPC-1 cells through DR3-mediated inhibition of NF-kappaB. Int J Cancer. 125:2465–2473. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Kim SC, Kang SH, Jeong SJ and Kim SH, Ko HS and Kim SH: Inhibition of c-Jun N-terminal kinase and nuclear factor κB pathways mediates fisetin-exerted anti-inflammatory activity in lipopolysccharide-treated RAW264. 7 cells. Immunopharmacol Immunotoxicol. 34:645–650. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Goh FY, Upton N, Guan S, Cheng C, Shanmugam MK, Sethi G, Leung BP and Wong WF: Fisetin, a bioactive flavonol, attenuates allergic airway inflammation through negative regulation of NF-κB. Eur J Pharmacol. 679:109–116. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Léotoing L, Wauquier F, Guicheux J, Miot-Noirault E, Wittrant Y and Coxam V: The polyphenol fisetin protects bone by repressing NF-κB and MKP-1-dependent signaling pathways in osteoclasts. PLoS One. 8:e683882013. View Article : Google Scholar : PubMed/NCBI

25 

Liao YC, Shih YW, Chao CH, Lee XY and Chiang TA: Involvement of the ERK signaling pathway in fisetin reduces invasion and migration in the human lung cancer cell line A549. J Agric Food Chem. 57:8933–8941. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Lin R, Maeda S, Liu C, Karin M and Edgington TS: A large noncoding RNA is a marker for murine hepatocellular carcinomas and a spectrum of human carcinomas. Oncogene. 26:851–858. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Li S, Dong P, Wang J, Zhang J, Gu J, Wu X, Wu W, Fei X, Zhang Z, Wang Y, et al: Icariin, a natural flavonol glycoside, induces apoptosis in human hepatoma SMMC-7721 cells via a ROS/JNK-dependent mitochondrial pathway. Cancer Lett. 298:222–230. 2010. View Article : Google Scholar : PubMed/NCBI

29 

Pal HC, Sharma S, Strickland LR, Katiyar SK, Ballestas ME, Athar M, Elmets CA and Afaq F: Fisetin inhibits human melanoma cell invasion through promotion of mesenchymal to epithelial transition and by targeting MAPK and NFκB signaling pathways. PLoS One. 9:e863382014. View Article : Google Scholar : PubMed/NCBI

30 

Ishikawa Y and Kitamura M: Dual potential of extracellular signal-regulated kinase for the control of cell survival. Biochem Biophys Res Commun. 264:696–701. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Ellinger-Ziegelbauer H, Brown K, Kelly K and Siebenlist U: Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 (MEKK) derivative. J Biol Chem. 272:2668–2674. 1997. View Article : Google Scholar : PubMed/NCBI

32 

Chou RH, Hsieh SC, Yu YL, Huang MH, Huang YC and Hsieh YH: Fisetin inhibits migration and invasion of human cervical cancer cells by down-regulating urokinase plasminogen activator expression through suppressing the p38 MAPK-dependent NF-κB signaling pathway. PLoS One. 8:e719832013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang J and Huang S: Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway. Exp Ther Med 15: 2667-2673, 2018.
APA
Wang, J., & Huang, S. (2018). Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway. Experimental and Therapeutic Medicine, 15, 2667-2673. https://doi.org/10.3892/etm.2017.5666
MLA
Wang, J., Huang, S."Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway". Experimental and Therapeutic Medicine 15.3 (2018): 2667-2673.
Chicago
Wang, J., Huang, S."Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2667-2673. https://doi.org/10.3892/etm.2017.5666
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J and Huang S: Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway. Exp Ther Med 15: 2667-2673, 2018.
APA
Wang, J., & Huang, S. (2018). Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway. Experimental and Therapeutic Medicine, 15, 2667-2673. https://doi.org/10.3892/etm.2017.5666
MLA
Wang, J., Huang, S."Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway". Experimental and Therapeutic Medicine 15.3 (2018): 2667-2673.
Chicago
Wang, J., Huang, S."Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2667-2673. https://doi.org/10.3892/etm.2017.5666
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