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Article

Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis

  • Authors:
    • Lili Liu
    • Dan Wang
    • Ying Qiu
    • Hongyan Dong
    • Xuemei Zhan
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China
  • Pages: 2655-2660
    |
    Published online on: December 22, 2017
       https://doi.org/10.3892/etm.2017.5675
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Abstract

Overcoming chemoresistance is a challenge in clinical treatment. It has been reported that microRNAs (miRNAs) are involved in regulating chemosensitivity. Therefore, the present study aimed to identify the effect and mechanism of miR‑15 on colon cancer chemotherapy. Reverse transcription‑quantitative polymerase chain reaction was performed to measure miR‑15 level sin62‑paired colon cancer and para‑cancerous colon tissues. The overexpression of miR‑15 in HCT116 cells was induced by transfection. The effect of miR‑15 on the chemosensitivity of colon cancer cells to 5‑fluorouracil (5‑FU) and Oxaliplatin (OX) was determined using a luminescent cell viability assay. Flow cytometry, dual‑luciferase assay and western blot analysis were used to determine the potential mechanism of miR‑15. The results suggested that the expression of miR‑15 was decreased in tumour tissues and that overexpression of miR‑15 increased the chemosensitivity of colon cancer cells to 5‑Fu and OX. miR‑15 promoted apoptosis in colon cancer cells treated with 5‑Fu and OX by inhibiting the expression of p50, which repressed the expression of B cell lymphoma‑2 and B cell lymphoma‑extra large; two direct target genes of nuclear factor‑κB with anti‑apoptotic functions. Thus, the current study demonstrated that miR‑15 increased the chemosensitivity of colon cancer cells to 5‑FU and OX by inhibiting the NF‑κB signalling pathway and inducing apoptosis.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Li L and Ma BB: Colorectal cancer in Chinese patients: Current and emerging treatment options. Onco Targets Ther. 7:1817–1828. 2014.PubMed/NCBI

3 

Loree JM and Kopetz S: Recent developments in the treatment of metastatic colorectal cancer. Ther Adv Med Oncol. 9:551–564. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Boland PM and Ma WW: Immunotherapy for colorectal cancer. Cancers. 9:pii: E502017. View Article : Google Scholar

5 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Ha M and Kim VN: Regulation of microRNA biogenesis. Nat Rev Mol Cell Biol. 15:509–524. 2014. View Article : Google Scholar : PubMed/NCBI

7 

King TD, Suto MJ and Li Y: The Wnt/β-catenin signaling pathway: A potential therapeutic target in the treatment of triple negative breast cancer. J Cell Biochem. 113:13–18. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Magee P, Shi L and Garofalo M: Role of microRNAs in chemoresistance. Ann Transl Med. 3:3322015.PubMed/NCBI

10 

Zhao C, Wang G, Zhu Y, Li X, Yan F, Zhang C, Huang X and Zhang Y: Aberrant regulation of miR-15b in human malignant tumors and its effects on the hallmarks of cancer. Tumour Biol. 37:177–183. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Zhang WL, Zhang JH, Wu XZ, Yan T and Lv W: miR-15b promotes epithelial-mesenchymal transition by inhibiting SMURF2 in pancreatic cancer. Int J Oncol. 47:1043–1053. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Lovat F, Fassan M, Gasparini P, Rizzotto L, Cascione L, Pizzi M, Vicentini C, Balatti V, Palmieri D, Costinean S and Croce CM: miR-15b/16-2 deletion promotes B-cell malignancies. Proc Natl Acad Sci USA. 112:pp. 11636–11641. 2015; View Article : Google Scholar : PubMed/NCBI

13 

Zhao Z, Zhang L, Yao Q and Tao Z: miR-15b regulates cisplatin resistance and metastasis by targeting PEBP4 in human lung adenocarcinoma cells. Cancer Gene Ther. 22:108–114. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Sun L, Yao Y, Liu B, Lin Z, Lin L, Yang M, Zhang W, Chen W, Pan C, Liu Q, et al: miR-200b and miR-15b regulate chemotherapy-induced epithelial-mesenchymal transition in human tongue cancer cells by targeting BMI1. Oncogene. 31:432–445. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Konishi T, Sasaki S, Watanabe T, Kitayama J and Nagawa H: Overexpression of hRFI inhibits 5-fluorouracil-induced apoptosis in colorectal cancer cells via activation of NF-kappaB and upregulation of BCL-2 and BCL-XL. Oncogene. 25:3160–3169. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Cimmino A, Calin GA, Fabbri M, Iorio MV, Ferracin M, Shimizu M, Wojcik SE, Aqeilan RI, Zupo S, Dono M, et al: miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA. 102:pp. 13944–13949. 2005; View Article : Google Scholar : PubMed/NCBI

18 

Cunningham D, Allum WH, Stenning SP, Thompson JN, Van de Velde CJ, Nicolson M, Scarffe JH, Lofts FJ, Falk SJ, Iveson TJ, et al: Perioperative chemotherapy versus surgery alone for resectable gastroesophageal cancer. N Engl J Med. 355:11–20. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Wilson TR, Longley DB and Johnston PG: Chemoresistance in solid tumours. Ann Oncol. 17 Suppl 10:x315–x324. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Gottesman MM, Fojo T and Bates SE: Multidrug resistance in cancer: Role of ATP-dependent transporters. Nat Rev Cancer. 2:48–58. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Barnes MJ, Estlin EJ, Taylor GA, Aherne GW, Hardcastle A, McGuire JJ, Calvete JA, Lunec J, Pearson AD and Newell DR: Impact of polyglutamation on sensitivity to raltitrexed and methotrexate in relation to drug-induced inhibition of de novo thymidylate and purine biosynthesis in CCRF-CEM cell lines. Clin Cancer Res. 5:2548–2558. 1999.PubMed/NCBI

22 

Dabholkar M, Vionnet J, Bostick-Bruton F, Yu JJ and Reed E: Messenger RNA levels of XPAC and ERCC1 in ovarian cancer tissue correlate with response to platinum-based chemotherapy. J Clin Invest. 94:703–708. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Amirkhah R, Farazmand A, Irfan-Maqsood M, Wolkenhauer O and Schmitz U: The role of microRNAs in the resistance to colorectal cancer treatments. Cell Mol Biol (Noisy-le-grand). 61:17–23. 2015.PubMed/NCBI

24 

Dehghanzadeh R, Jadidi-Niaragh F, Gharibi T and Yousefi M: MicroRNA-induced drug resistance in gastric cancer. Biomed Pharmacother. 74:191–199. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Wang G, Zhao R, Zhao X, Chen XI, Wang D, Jin Y, Liu XI, Zhao CI, Zhu Y, Ren C, et al: MicroRNA-181a enhances the chemotherapeutic sensitivity of chronic myeloid leukemia to imatinib. Oncol Lett. 10:2835–2841. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Yang H, Kong W, He L, Zhao JJ, O'Donnell JD, Wang J, Wenham RM, Coppola D, Kruk PA, Nicosia SV and Cheng JQ: MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Res. 68:425–433. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Chen X, Jiang Y, Huang Z, Li D, Chen X, Cao M, Meng Q, Pang H, Sun L, Zhao Y and Cai L: miRNA-378 reverses chemoresistance to cisplatin in lung adenocarcinoma cells by targeting secreted clusterin. Sci Rep. 6:194552016. View Article : Google Scholar : PubMed/NCBI

28 

Xi Y, Formentini A, Chien M, Weir DB, Russo JJ and Ju J, Kornmann M and Ju J: Prognostic values of microRNAs in colorectal cancer. Biomarker Insights. 2:113–121. 2006.PubMed/NCBI

29 

Wang X, Wang J, Ma H, Zhang J and Zhou X: Downregulation of miR-195 correlates with lymph node metastasis and poor prognosis in colorectal cancer. Med Oncol. 29:919–927. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Wu CS, Yen CJ, Chou RH, Chen JN, Huang WC, Wu CY and Yu YL: Downregulation of microRNA-15b by hepatitis B virus X enhances hepatocellular carcinoma proliferation via fucosyltransferase 2-induced Globo H expression. Int J Cancer. 134:1638–1647. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Kedmi M, Ben-Chetrit N, Körner C, Mancini M, Ben-Moshe NB, Lauriola M, Lavi S, Biagioni F, Carvalho S, Cohen-Dvashi H, et al: EGF induces microRNAs that target suppressors of cell migration: miR-15b targets MTSS1 in breast cancer. Sci Signal. 8:ra292015. View Article : Google Scholar : PubMed/NCBI

32 

Zheng X, Chopp M, Lu Y, Buller B and Jiang F: miR-15b and miR-152 reduce glioma cell invasion and angiogenesis via NRP-2 and MMP-3. Cancer Lett. 329:146–154. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Li J, Chen Y, Guo X, Zhou L, Jia Z, Tang Y, Lin L, Liu W and Ren C: Inhibition of miR-15b decreases cell migration and metastasis in colorectal cancer. Tumour Biol. 37:8765–8773. 2016. View Article : Google Scholar : PubMed/NCBI

34 

Xia L, Zhang D, Du R, Pan Y, Zhao L, Sun S, Hong L, Liu J and Fan D: miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer. 123:372–379. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Rayet B and Gelinas C: Aberrant rel/nfkb genes and activity in human cancer. Oncogene. 18:6938–6947. 1999. View Article : Google Scholar : PubMed/NCBI

36 

Barkett M and Gilmore TD: Control of apoptosis by Rel/NF-kappaB transcription factors. Oncogene. 18:6910–6924. 1999. View Article : Google Scholar : PubMed/NCBI

37 

Cheng Q, Lee HH, Li Y, Parks TP and Cheng G: Upregulation of Bcl-x and Bfl-1 as a potential mechanism of chemoresistance, which can be overcome by NF-kappaB inhibition. Oncogene. 19:4936–4940. 2000. View Article : Google Scholar : PubMed/NCBI

38 

Xia Y, Shen S and Verma IM: NF-κB, an active player in human cancers. Cancer Immunol Res. 2:823–830. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Wang D, Qiu Y, Dong H and Zhan X: Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Exp Ther Med 15: 2655-2660, 2018.
APA
Liu, L., Wang, D., Qiu, Y., Dong, H., & Zhan, X. (2018). Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Experimental and Therapeutic Medicine, 15, 2655-2660. https://doi.org/10.3892/etm.2017.5675
MLA
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15.3 (2018): 2655-2660.
Chicago
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2655-2660. https://doi.org/10.3892/etm.2017.5675
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Wang D, Qiu Y, Dong H and Zhan X: Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Exp Ther Med 15: 2655-2660, 2018.
APA
Liu, L., Wang, D., Qiu, Y., Dong, H., & Zhan, X. (2018). Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis. Experimental and Therapeutic Medicine, 15, 2655-2660. https://doi.org/10.3892/etm.2017.5675
MLA
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15.3 (2018): 2655-2660.
Chicago
Liu, L., Wang, D., Qiu, Y., Dong, H., Zhan, X."Overexpression of microRNA-15 increases the chemosensitivity of colon cancer cells to 5-fluorouracil and oxaliplatin by inhibiting the nuclear factor-κB signalling pathway and inducing apoptosis". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2655-2660. https://doi.org/10.3892/etm.2017.5675
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