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Article

Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection

  • Authors:
    • Xiaohong Zhang
    • Tie Zhao
    • Tiebing Zeng
    • Ning Wu
    • Yongjian Xiao
    • Shuangquan Liu
    • Jian Yu
    • Chuanhao Jiang
    • Lin Gan
    • Meixia Deng
    • Xi Luo
    • Feijun Zhao
  • View Affiliations / Copyright

    Affiliations: Institute of Pathogenic Biology and Key Laboratory of Special Pathogen Prevention and Control of Hunan Province, University of South China, Hengyang, Hunan 421001, P.R. China, Department of Clinical Laboratory, Hengyang No. 1 People's Hospital, Hengyang, Hunan 421001, P.R. China, Department of Clinical Laboratory, The Second Hospital Affiliated to University of South China, Hengyang, Hunan 421001, P.R. China, Department of Clinical Laboratory, The First Hospital Affiliated to University of South China, Hengyang, Hunan 421001, P.R. China, Department of Laboratory Animals, University of South China, Hengyang, Hunan 421001, P.R. China
  • Pages: 2533-2540
    |
    Published online on: January 4, 2018
       https://doi.org/10.3892/etm.2018.5689
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Abstract

The present study aimed to evaluate the immune effect of intramuscular primary immunization by the nucleic acid vaccine pcDNA/glycerophosphodiester phosphodiesterase‑interleukin‑2 (pcDNA/Gpd‑IL‑2) and enhanced immunization 2 weeks later with the combination of mucosal adjuvant CpG‑oligodeoxynucleotides (ODN) and Gpd‑IL‑2 recombinant protein on skin infection caused by Treponema pallidum (Tp) in New Zealand rabbits. At week 8 following immunization, MTT assay was used to detect spleen cell proliferation, while enzyme‑linked immunosorbent assay was performed to detect the cytokine and secretory immunoglobulin A (SIgA) levels. At week 10 after primary immunization, rabbits were inoculated with 105 Tp (Nichols strain). Alterations in the skin redness, swelling and ulceration were recorded for 0‑60 days. In addition, positive rate of Tp in skin lesions and ulcer formation rate were examined using dark field and silver staining. The results indicated that intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 followed by enhanced immunization via nasal feeding with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein induced the higher levels of Tp Gpd specific antibodies, increased the secretion of IL‑2 and interferon‑γ, and promoted the proliferation of T cells in the first 8 weeks after immunization. Furthermore, this immunization strategy stimulated the production of mucosa specific SIgA antibody. Thus, this strategy led to the lowest Tp positive and ulcer formation rates at the Tp infection sites, as well as healing of skin lesions on the earliest time point (day 42). In conclusion, immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 followed by enhanced immunization with a combination of mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein is an effective immune strategy to induce strong mucosal immune responses and immune protective effects.
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1 

Shah BJ, Karia DR and Pawara CL: Syphilis: Is it making resurgence? Indian J Sex Transm Dis. 36:178–181. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Wang L, Zeng L, Ren X, Geng M, Li Z and Yu H: Analysis of morbidity and mortality characteristics of the notifiable diseases reported in China. Zhonghua Liu Xing Bing Xue Za Zhi. 36:194–198. 2015.(In Chinese). PubMed/NCBI

3 

Zheng N, Guo Y, Padmadas S, Wang B and Wu Z: The increase of sexually transmitted infections calls for simultaneous preventive intervention for more effectively containing HIV epidemics in China. BJOG. 121:35–44. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Hiramatsu K, Serada S, Kobiyama K, Nakagawa S, Morimoto A, Matsuzaki S, Ueda Y, Fujimoto M, Yoshino K, Ishii KJ, et al: CpG oligodeoxynucleotides potentiate the antitumor activity of anti-BST2 antibody. Cancer Sci. 106:1474–1478. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Li HT, Zhang TT, Chen ZG, Ye J, Liu H, Zou XL, Wang YH and Yang HL: Intranasal administration of CpG oligodeoxynucleotides reduces lower airway inflammation in a murine model of combined allergic rhinitis and asthma syndrome. Int Immunopharmacol. 28:390–398. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Iho S, Maeyama J and Suzuki F: CpG oligodeoxynucleotides as mucosal adjuvants. Hum Vaccin Immunother. 11:755–760. 2015. View Article : Google Scholar : PubMed/NCBI

7 

Dai Y, Zhu Y, Harn DA, Wang X, Tang J, Zhao S, Lu F and Guan X: DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica. Clin Vaccine Immunol. 16:1796–1803. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Bae JY, Moon SH, Choi JA, Park JS, Hahn BS, Kim KY, Kim B, Song JY, Kwon DH, Lee SC, et al: Recombinant DNA and protein vaccines for foot-and-mouth disease induce humoral and cellular immune responses in mice. Immune Netw. 9:265–273. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Endmann A, Klünder K, Kapp K, Riede O, Oswald D, Talman EG, Schroff M, Kleuss C, Ruiters MH and Juhls C: Cationic lipid-formulated DNA vaccine against hepatitis B virus: Immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine. PLoS One. 9:e1017152014. View Article : Google Scholar : PubMed/NCBI

10 

Nchinda G, Amadu D, Trumpfheller C, Mizenina O, Uberla K and Steinman RM: Dendritic cell targeted HIV gag protein vaccine provides help to a DNA vaccine including mobilization of protective CD8+ T cells. Proc Natl Acad Sci USA. 107:pp. 4281–4286. 2010; View Article : Google Scholar : PubMed/NCBI

11 

Li W, Wang S and Lu S: Pilot study on the use of DNA priming immunization to Enhance Y. pestis LcrV-Specific B cell responses elicited by a recombinant LcrV protein vaccine. Vaccines (Basel). 2:36–48. 2014. View Article : Google Scholar

12 

Zhao F, Wang S, Zhang X, Gu W, Yu J, Liu S, Zeng T, Zhang Y and Wu Y: Protective efficacy of a Treponema pallidum Gpd DNA vaccine vectored by chitosan nanoparticles and fused with interleukin-2. Can J Microbiol. 58:117–123. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Zhao F, Liu S, Zhang X, Yu J, Zeng T, Gu W, Cao X, Chen X and Wu Y: CpG adjuvant enhances the mucosal immunogenicity and efficacy of a Treponema pallidum DNA vaccine in rabbits. Hum Vaccin Immunother. 9:753–760. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Zhao Feijun, Zhang Xiaohong, Yu Jin, Liu Shuangquan, Zhang Yuejun and Wu Yimou: Expression and identification of Gpd-IL-2 recombinant protein of treponema pallidum and its immuno-competence analysis. J Med Sci Central South China. 40:131–135. 2012.(In Chinese).

15 

Syphilis. Nurs Stand. 29:172015. View Article : Google Scholar

16 

Janier M, Hegyi V, Dupin N, Unemo M, Tiplica GS, Potočnik M, French P and Patel R: 2014 European guideline on the management of syphilis. J Eur Acad Dermatol Venereol. 28:1581–1593. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Stamm LV and Drapp RL: A synthetic lymph node containing inactivated Treponema pallidum cells elicits strong, antigen-specifichumoral and cellular immune responses in mice. Pathog Dis. 70:88–94. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Khera AK, Afkhami S, Lai R, Jeyanathan M, Zganiacz A, Mandur T, Hammill J, Damjanovic D and Xing Z: Role of B cells in mucosal vaccine-induced protective CD8+ T cell immunity against pulmonary tuberculosis. J Immunol. 195:2900–2907. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Stary G, Olive A, Radovic-Moreno AF, Gondek D, Alvarez D, Basto PA, Perro M, Vrbanac VD, Tager AM, Shi J, et al: VACCINES. A mucosal vaccine against Chlamydia trachomatis generates two waves of protective memory T cells. Science. 348:aaa82052015. View Article : Google Scholar : PubMed/NCBI

20 

Ye T, Yue Y, Fan X, Dong C, Xu W and Xiong S: M cell-targeting strategy facilitates mucosal immune response and enhances protection against CVB3-induced viral myocarditis elicited by chitosan-DNA vaccine. Vaccine. 32:4457–4465. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Umthong S, Buaklin A, Jacquet A, Sangjun N, Kerdkaew R, Patarakul K and Palaga T: Immunogenicity of a DNA and recombinant protein vaccine combining LipL32 and Loa22 for leptospirosis using chitosan as a delivery system. J Microbiol Biotechnol. 25:526–536. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Birk R, Aderhold C, Hörmann K, Wenzel A, Kramer B, Eschenhagen T and Sommer JU: CpG-Oligodeoxynucleotides in Chronic Rhinosinusitis Cell Culture. In Vivo. 30:47–52. 2016.PubMed/NCBI

23 

Meng Z, Zhang X, Pei R, Zhang E, Kemper T, Vollmer J, Davis HL, Glebe D, Gerlich W, Roggendorf M, et al: Combination therapy including CpG oligodeoxynucleotides and entecavir induces early viral response and enhanced inhibition of viral replication in a woodchuck model of chronic hepadnaviral infection. Antiviral Res. 125:14–24. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Xiang XX, Zhou XQ, Wang JX, Xie Q, Cai X, Yu H and Zhou HJ: Effects of CpG-ODNs on phenotype and function of monocyte-derived dendritic cells in chronic hepatitis B. World J Gastroenterol. 17:4825–4830. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Lei J, Osen W, Gardyan A, Hotz-Wagenblatt A, Wei G, Gissmann L, Eichmüller S and Löchelt M: Replication-competent foamy virus vaccine vectors as novel epitope scaffolds for immunotherapy. PLoS One. 10:e01384582015. View Article : Google Scholar : PubMed/NCBI

26 

San R, Omán B, Gómez S, Irache JM and Espuelas S: Co-encapsulated CpG oligodeoxynucleotides and ovalbumin in PLGA microparticles; an in vitro and in vivo study. J Pharm Pharm Sci. 17:541–553. 2014. View Article : Google Scholar : PubMed/NCBI

27 

De Cesare M, Sfondrini L, Pennati M, De Marco C, Motta V, Tagliabue E, Deraco M, Balsari A and Zaffaroni N: CpG-oligodeoxynucleotides exert remarkable antitumor activity against diffuse malignant peritoneal mesothelioma orthotopic xenografts. J Transl Med. 14:252016. View Article : Google Scholar : PubMed/NCBI

28 

Zhang C, Zheng M, Zhu XH, Li S, Ni J, Li BL, Liu H, Gao F and Cai JM: Protective effect of CpG-oligodeoxynucleotides against low- and high-LET irradiation. Cell Physiol Biochem. 34:1663–1674. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Zhao T, Zeng T, Wu N, Xiao Y, Liu S, Yu J, Jiang C, Gan L, Deng M, Deng M, et al: Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection. Exp Ther Med 15: 2533-2540, 2018.
APA
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S. ... Zhao, F. (2018). Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection. Experimental and Therapeutic Medicine, 15, 2533-2540. https://doi.org/10.3892/etm.2018.5689
MLA
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S., Yu, J., Jiang, C., Gan, L., Deng, M., Luo, X., Zhao, F."Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection". Experimental and Therapeutic Medicine 15.3 (2018): 2533-2540.
Chicago
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S., Yu, J., Jiang, C., Gan, L., Deng, M., Luo, X., Zhao, F."Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2533-2540. https://doi.org/10.3892/etm.2018.5689
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Zhao T, Zeng T, Wu N, Xiao Y, Liu S, Yu J, Jiang C, Gan L, Deng M, Deng M, et al: Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection. Exp Ther Med 15: 2533-2540, 2018.
APA
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S. ... Zhao, F. (2018). Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection. Experimental and Therapeutic Medicine, 15, 2533-2540. https://doi.org/10.3892/etm.2018.5689
MLA
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S., Yu, J., Jiang, C., Gan, L., Deng, M., Luo, X., Zhao, F."Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection". Experimental and Therapeutic Medicine 15.3 (2018): 2533-2540.
Chicago
Zhang, X., Zhao, T., Zeng, T., Wu, N., Xiao, Y., Liu, S., Yu, J., Jiang, C., Gan, L., Deng, M., Luo, X., Zhao, F."Intramuscular primary immunization by nucleic acid vaccine pcDNA/Gpd‑IL‑2 and enhanced immunization with mucosal adjuvant CpG‑ODN and Gpd‑IL‑2 recombinant protein effectively induced strong mucosal immune responses and immune protective effects against Treponema pallidum skin infection". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2533-2540. https://doi.org/10.3892/etm.2018.5689
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