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Article

PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells

  • Authors:
    • Shuiling Jin
    • Yanfang Mi
    • Jing Song
    • Peipei Zhang
    • Yanyan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450014, P.R. China, Department of Internal Medicine, Henan Cancer Hospital and Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan 450008, P.R. China
  • Pages: 2563-2568
    |
    Published online on: January 4, 2018
       https://doi.org/10.3892/etm.2018.5693
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Abstract

Protein arginine methyltransferase 1 (PRMT1) serves pivotal roles in various cellular processes. However, its role in megakaryocytic differentiation has not been clearly reported. The aim of the present study was to explore the role of the PRMT‑RNA binding motif protein 15 (RBM15) axis in human MK differentiation and the feasibility of targeting PRMT1 for leukemia treatment. In the present study, PRMT1 was overexpressed and the RBM15 protein was knocked down in human umbilical cord blood cluster of differentiation (CD)34+ cells and the cells were then cultured in megakaryocytic differentiation medium. Flow cytometry was used to analyze CD41 and CD42 double‑positive cells, as well as the protein expression levels of PRMT1 and RBM15. The results demonstrated that human cord blood CD34+ cells differentiate into mature MKs in high thrombopoitin medium, as demonstrated by CD41 and CD42 expression. Overexpression of PRMT1 in human umbilical cord blood CD34+ cells blocked the maturation of megakaryocytic cells. Knockdown of RBM15 by short hairpin RNA produced less mature MKs. PRMT1 inhibitor rescued PRMT1‑blocked megakaryocytic differentiation. These results provide evidence for a novel role of PRMT1 in the negative regulation of megakaryocytic differentiation. PRMT1 may be a therapeutic target for leukemia treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Jin S, Mi Y, Song J, Zhang P and Liu Y: PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Exp Ther Med 15: 2563-2568, 2018.
APA
Jin, S., Mi, Y., Song, J., Zhang, P., & Liu, Y. (2018). PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Experimental and Therapeutic Medicine, 15, 2563-2568. https://doi.org/10.3892/etm.2018.5693
MLA
Jin, S., Mi, Y., Song, J., Zhang, P., Liu, Y."PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells". Experimental and Therapeutic Medicine 15.3 (2018): 2563-2568.
Chicago
Jin, S., Mi, Y., Song, J., Zhang, P., Liu, Y."PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2563-2568. https://doi.org/10.3892/etm.2018.5693
Copy and paste a formatted citation
x
Spandidos Publications style
Jin S, Mi Y, Song J, Zhang P and Liu Y: PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Exp Ther Med 15: 2563-2568, 2018.
APA
Jin, S., Mi, Y., Song, J., Zhang, P., & Liu, Y. (2018). PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells. Experimental and Therapeutic Medicine, 15, 2563-2568. https://doi.org/10.3892/etm.2018.5693
MLA
Jin, S., Mi, Y., Song, J., Zhang, P., Liu, Y."PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells". Experimental and Therapeutic Medicine 15.3 (2018): 2563-2568.
Chicago
Jin, S., Mi, Y., Song, J., Zhang, P., Liu, Y."PRMT1-RBM15 axis regulates megakaryocytic differentiation of human umbilical cord blood CD34+ cells". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2563-2568. https://doi.org/10.3892/etm.2018.5693
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