The role of miR-766-5p in cell migration and invasion in colorectal cancer

Jia,Bin; Xia,Lei; Cao,Fang

doi:10.3892/etm.2018.5716

The role of miR-766-5p in cell migration and invasion in colorectal cancer

Authors:
- Bin Jia
- Lei Xia
- Fang Cao
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Affiliations: Department of Gastroenterology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China, Department of Oncology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250014, P.R. China
Published online on: January 8, 2018 https://doi.org/10.3892/etm.2018.5716
Pages: 2569-2574

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Abstract

Colorectal cancer (CRC) develops from the colon or rectum and is the fourth highest inducer of cancer mortality. In the present study, cancer tissues and normal tissues were extracted from patients with CRC who were treated in the Affiliated Hospital of Shandong University of Traditional Chinese Medicine (Jinan, China). Reverse transcription-quantitative polymerase chain reaction demonstrated that the expression level of miR‑766‑5p was significantly higher (P<0.01) in cancer tissue than that in normal tissue. SW480 cells were used for in vitro study and randomly separated into the miR‑negative control (NC) inhibitor treatment group and miR‑766‑5p inhibitor treatment group. SW480 cell behaviors were evaluated. Results demonstrated that in the miR‑766‑5p inhibitor group, there was a decreased level of cell proliferation/migration/invasion and higher cell apoptosis compared with that in the miR‑NC inhibitor group. miR‑766‑5p was predicted and verified to target the 3' untranslated region of suppressor of cancer cell invasion (SCAI) in SW480 cells. Protein expression levels of matrix metalloproteinase‑2/phosphoinositide 3‑kinase/AKT were decreased and SCAI was increased following miR‑766‑5p inhibitor treatment. In conclusion, the present study indicated that miR‑766‑5p inhibitor repressed the process of CRC by targeting SCAI.

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