Open Access

Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury

  • Authors:
    • Xuewen Su
    • Wenyan Tang
    • Zuo Luan
    • Yinxiang Yang
    • Zhaoyan Wang
    • Yu Zhang
    • Qian Wang
    • Lei Suo
    • Zhen Huang
    • Xiue Wang
    • Haifeng Yuan
  • View Affiliations

  • Published online on: January 8, 2018     https://doi.org/10.3892/etm.2018.5717
  • Pages: 2443-2449
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the protective effects of miconazole on myelin sheaths following cerebral white matter damage (WMD) in premature infant rats. Sprague Dawley rats (3‑days‑old) were randomly divided into four groups (n=30 each) as follows: Sham surgery group, WMD model group, 10 mg/kg/day treatment group and 40 mg/kg/day treatment group. A cerebral white matter lesion model was created by ligating the right common carotid artery for 80 min. Treatment groups were administered with 10 or 40 mg/kg miconazole at 4‑8 days following birth (early treatment group) or 5‑11 days following birth (late treatment group). Rats in the model group received the same concentration of dimethylsulfoxide. Myelin basic protein (MBP) immunohistochemical staining and western blotting were used to detect the expression of cerebral white matter‑specific MBP, and changes in myelin structure were observed using transmission electron microscopy. No swelling or necrosis was observed in the corpus callosum of the sham group rats, whereas rats in the model group demonstrated edema, loose structure, fiber disorder, inflammatory gliocytes and selective white matter lesions. Following treatment with miconazole, MBP expression in the corpus callosum was significantly higher compared with the model group. Furthermore, in the model group, myelin sheaths in the corpus callosum were loose with small vacuoles, there was a marked decrease in thickness and structural damage was observed. Conversely, a marked improvement in myelination was observed in the treatment group. The results of the present study suggest that miconazole is able to promote formation of the myelin sheath to ameliorate premature cerebral white matter lesions caused by ischemia or hypoxia in rats.
View Figures
View References

Related Articles

Journal Cover

March-2018
Volume 15 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Su X, Tang W, Luan Z, Yang Y, Wang Z, Zhang Y, Wang Q, Suo L, Huang Z, Wang X, Wang X, et al: Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury. Exp Ther Med 15: 2443-2449, 2018
APA
Su, X., Tang, W., Luan, Z., Yang, Y., Wang, Z., Zhang, Y. ... Yuan, H. (2018). Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury. Experimental and Therapeutic Medicine, 15, 2443-2449. https://doi.org/10.3892/etm.2018.5717
MLA
Su, X., Tang, W., Luan, Z., Yang, Y., Wang, Z., Zhang, Y., Wang, Q., Suo, L., Huang, Z., Wang, X., Yuan, H."Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury". Experimental and Therapeutic Medicine 15.3 (2018): 2443-2449.
Chicago
Su, X., Tang, W., Luan, Z., Yang, Y., Wang, Z., Zhang, Y., Wang, Q., Suo, L., Huang, Z., Wang, X., Yuan, H."Protective effect of miconazole on rat myelin sheaths following premature infant cerebral white matter injury". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2443-2449. https://doi.org/10.3892/etm.2018.5717