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Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway

  • Authors:
    • Jiang Wei
    • Yang Wenjie
    • Liu Ping
    • Wang Na
    • Ren Haixia
    • Zhao Xuequn
  • View Affiliations / Copyright

    Affiliations: Department of Infectious Disease, Tianjin First Center Hospital, Tianjin 300192, P.R. China, Laboratory of Microbiology of Tianjin First Center Hospital, Tianjin 300192, P.R. China, Department of Transplantation, Tianjin First Center Hospital, Tianjin 300192, P.R. China, Department of Pharmacy, Tianjin First Center Hospital, Tianjin 300192, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2247-2254
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    Published online on: January 9, 2018
       https://doi.org/10.3892/etm.2018.5728
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Abstract

Overuse and misuse of antibiotics leads to rapid evolution of antibiotic‑resistant bacteria and antibiotic resistance genes. Klebsiella pneumoniae has become the most common pathogenic bacterium accountable for nosocomial infections due to its high virulence factor and general occurrence of resistance to most antibiotics. The β‑lactamase signaling pathway has been suggested to be involved in antibiotic resistance against β‑lactams in Klebsiella pneumoniae. In the present study, the molecular mechanism of the antibiotic resistance of Klebsiella pneumoniae was investigated and the results indicated involvement of the β‑arrestin recruitment‑induced β‑lactamase signaling pathway. Antimicrobial susceptibility of Klebsiella pneumoniae was assessed using automated systems and extended‑spectrum β‑lactamase (ESBL) and β‑arrestin expression levels in Klebsiella pneumoniae were analyzed by reverse‑transcription quantitative PCR. β‑lactam resistance in Klebsiella pneumoniae was determined using β‑lactam agar screening plates. The results demonstrated that β‑arrestin recruitment was increased in Klebsiella pneumoniae with antibiotic resistance (AR‑K.P.) compared with that in the native Klebsiella pneumoniae strain (NB‑K.P.). Increased production of ESBL was observed in AR‑K.P. after treatment with the β‑lactam penicillin. Of note, inhibition of β‑arrestin recruitment significantly suppressed ESBL expression in AR‑K.P. and in addition, genes encoding β‑arrestin and ESBL were upregulated in Klebsiella pneumoniae. Restoration of endogenous β‑arrestin markedly increased antibiotic resistance of Klebsiella pneumoniae to β‑lactam. Knockdown of endogenous β‑arrestin downregulated antibiotic resistance genes and promoted the inhibitory effects of β‑lactam antibiotic treatment on Klebsiella pneumoniae growth. In conclusion, the present study identified that β‑arrestin recruitment was associated with growth and resistance to β‑lactams, which suggested that β‑arrestin regulating ESBL expression may be a potential target for addressing antibiotic resistance to β‑lactams in Klebsiella pneumoniae.
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Spandidos Publications style
Wei J, Wenjie Y, Ping L, Na W, Haixia R and Xuequn Z: Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway. Exp Ther Med 15: 2247-2254, 2018.
APA
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., & Xuequn, Z. (2018). Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway. Experimental and Therapeutic Medicine, 15, 2247-2254. https://doi.org/10.3892/etm.2018.5728
MLA
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., Xuequn, Z."Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway". Experimental and Therapeutic Medicine 15.3 (2018): 2247-2254.
Chicago
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., Xuequn, Z."Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2247-2254. https://doi.org/10.3892/etm.2018.5728
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Spandidos Publications style
Wei J, Wenjie Y, Ping L, Na W, Haixia R and Xuequn Z: Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway. Exp Ther Med 15: 2247-2254, 2018.
APA
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., & Xuequn, Z. (2018). Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway. Experimental and Therapeutic Medicine, 15, 2247-2254. https://doi.org/10.3892/etm.2018.5728
MLA
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., Xuequn, Z."Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway". Experimental and Therapeutic Medicine 15.3 (2018): 2247-2254.
Chicago
Wei, J., Wenjie, Y., Ping, L., Na, W., Haixia, R., Xuequn, Z."Antibiotic resistance of Klebsiella pneumoniae through β-arrestin recruitment-induced β-lactamase signaling pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2247-2254. https://doi.org/10.3892/etm.2018.5728
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