Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer

Wang,Yan; Li,Jiao‑Jiao; Chen,Yu‑Min

doi:10.3892/etm.2018.5731

Biochanin A extirpates the epithelial‑mesenchymal transition in a human lung cancer

Authors:
- Yan Wang
- Jiao‑Jiao Li
- Yu‑Min Chen
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Affiliations: Department of Cardiovascular and Thoracic Surgery, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Intensive Care Unit, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
Published online on: January 10, 2018 https://doi.org/10.3892/etm.2018.5731
Pages: 2830-2836
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The natural iso-flavonoid, biochanin A, is categorized as a phytoestrogen and has been demonstrated to exhibit various pharmacological properties. However, no effects of biochanin A on lung cancer cell lines have been reported. Therefore, the present study aimed to demonstrate whether biochanin A has the ability to reduce lung cancer triggered pro‑inflammatory effects from leukemic monocytes. We studied the release of cytokines, tumor necrosis factor (TNF)‑α and interleukin (IL)‑6, from the cocultured cells of A427:AML‑193. In addition to this, epithelial‑mesenchymal transition was also monitored. In the cocultured A427 and AML‑193, AML‑193 was stimulated by A427 cells assisting the release of TNF‑α and IL‑6 cytokines, but the addition of A427 with biochanin A reduced A427‑triggered generation of cytokines by AML‑193. Moreover, this non‑functional A427:AML‑193 coculture reduced the metastasis effects of A427 cells, as determined by wound healing assays and migration/invasion assays. These results were further confirmed by a reduction in Snail and E‑cadherin expression levels, which are indicators of the epithelial‑mesenchymal transition. These findings suggest the therapeutic effect of biochanin A against lung cancer evoked inflammation and pro‑inflammatory functions from monocytic cells.

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