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Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation

  • Authors:
    • Shenglong Li
    • Jing Wang
    • Yudi Han
    • Xiaoteng Li
    • Changjian Liu
    • Zhengshuai Lv
    • Xiuhui Wang
    • Xin Tang
    • Zhe Wang
  • View Affiliations / Copyright

    Affiliations: Department of Bone and Soft Tissue Tumor Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China, Department of Plastic and Cosmetic Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Plastic and Reconstructive Surgery, General Hospital of Chinese PLA, Beijing 100853, P.R. China, Department of Orthopedic Trauma, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China, Department of Orthopedics, Shanghai Zhoupu Hospital, Shanghai 201318, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2798-2803
    |
    Published online on: January 17, 2018
       https://doi.org/10.3892/etm.2018.5757
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Abstract

The aim of the present study was to explore the effects of pannexin1 (Px1) protein channels on osteogenic differentiation of mesenchymal stem cells (MSCs) under mechanical stress stimulation. MSCs were isolated from Sprague Dawley rats (3 weeks old, weighing 100‑120 g) and cultured in vitro. A safe concentration of carbenoxolone was determined (CBX, an inhibitor of Px1 channels; 100 µM) on MSCs using the Cell Counting Kit‑8 (CCK8) method. MSCs were divided into 6 groups: Control, stress (4,000 µ strain), and stress following 3, 6, 12, and 24 h pretreatment with CBX. Stress groups were stimulated with mechanical stress for 15 min. Alkaline phosphatase (ALP) activity, type I collagen expression, intracellular calcium ion (Ca2+) concentration, Px1 expression, p38 mitogen‑activated protein kinase (MAPK) and extracellular signal‑regulated phosphorylation were determined. ALP activity was increased in the stress group, and this was prevented by pretreatment with CBX. Similarly, stress‑induced increases in type I collagen expression, Ca2+ concentration, Px1 expression, and p38 MAPK phosphorylation decreased in the presence of CBX. ERK phosphorylation was decreased by stress, however was not affected by CBX treatment. Altogether, the results suggest that mechanical stress promoted the osteogenic differentiation of MSCs, and this promotion was inhibited by pretreatment with CBX, possibly through regulating the phosphorylation of p38 MAPK.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Wang J, Han Y, Li X, Liu C, Lv Z, Wang X, Tang X and Wang Z: Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Exp Ther Med 15: 2798-2803, 2018.
APA
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z. ... Wang, Z. (2018). Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Experimental and Therapeutic Medicine, 15, 2798-2803. https://doi.org/10.3892/etm.2018.5757
MLA
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z., Wang, X., Tang, X., Wang, Z."Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation". Experimental and Therapeutic Medicine 15.3 (2018): 2798-2803.
Chicago
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z., Wang, X., Tang, X., Wang, Z."Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2798-2803. https://doi.org/10.3892/etm.2018.5757
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Wang J, Han Y, Li X, Liu C, Lv Z, Wang X, Tang X and Wang Z: Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Exp Ther Med 15: 2798-2803, 2018.
APA
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z. ... Wang, Z. (2018). Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation. Experimental and Therapeutic Medicine, 15, 2798-2803. https://doi.org/10.3892/etm.2018.5757
MLA
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z., Wang, X., Tang, X., Wang, Z."Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation". Experimental and Therapeutic Medicine 15.3 (2018): 2798-2803.
Chicago
Li, S., Wang, J., Han, Y., Li, X., Liu, C., Lv, Z., Wang, X., Tang, X., Wang, Z."Carbenoxolone inhibits mechanical stress-induced osteogenic differentiation of mesenchymal stem cells by regulating p38 MAPK phosphorylation". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2798-2803. https://doi.org/10.3892/etm.2018.5757
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