Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
March-2018 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2018 Volume 15 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia

  • Authors:
    • Houyang Chen
    • Tao Luo
    • Tingtao Chen
    • Gongxian Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Institute of Life Science, Nanchang University, Nanchang, Jiangxi 330031, P.R. China, Institute of Translational Medicine, Nanchang University, Nanchang, Jiangxi 330031, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2884-2890
    |
    Published online on: January 19, 2018
       https://doi.org/10.3892/etm.2018.5778
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non‑obstructive azoospermia. A high‑throughput sequencing method was adopted to sequence genomic DNA extracted from the semen of healthy people (C group), patients with obstructive azoospermia (OA group) and patients with non‑obstructive azoospermia (NOA group). The results revealed that Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria species comprised the majority of bacteria in the C (98.14%), OA (98.26%) and NOA (90.96%) groups. Patients in the OA and NOA groups exhibited an increase in Bacteroidetes and Firmicutes, whereas the number of Proteobacteria and Actinobacteria were decreased compared with the C group. A total of 398 common operational taxonomic units were identified, of which 27 belonged to the genus Lactobacillus. Furthermore, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States analysis indicated that the pathogenic species and reduced biodiversity in the semen of patients with azoospermia may result in an increased risk of metabolic, infectious and immune diseases. In the present study, the seminal microbiome of patients with obstructive or non‑obstructive azoospermia was explored, which may be useful for developing novel treatments against azoospermia as well as for its diagnosis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Luo T, Zou QX, He YQ, Wang HF, Wang T, Liu M, Chen Y and Wang B: Matrine compromises mouse sperm functions by a [Ca(2+)]i-related mechanism. Reprod Toxicol. 60:69–75. 2016. View Article : Google Scholar : PubMed/NCBI

2 

He Y, Zou Q, Li B, Chen H, Du X, Weng S, Luo T and Zeng X: Ketamine inhibits human sperm function by Ca(2+)-related mechanism. Biochem Biophys Res Commun. 478:501–506. 2016. View Article : Google Scholar : PubMed/NCBI

3 

He Y, Zou Q, Chen H, Weng S, Luo T and Zeng X: Lead inhibits human sperm functions by reducing the levels of intracellular calcium, cAMP and tyrosine phosphorylation. Tohoku J Exp Med. 238:295–303. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Hooper LV, Littman DR and Macpherson AJ: Interactions between the microbiota and the immune system. Science. 336:1268–1273. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Nelson MH, Diven MA, Huff LW and Paulos CM: Harnessing the microbiome to enhance cancer immunotherapy. J Immunol Res. 2015:3687362015. View Article : Google Scholar : PubMed/NCBI

6 

Nakatsuji T, Chen TH, Narala S, Chun KA, Two AM, Yun T, Shafiq F, Kotol PF, Bouslimani A, Melnik AV, et al: Antimicrobials from human skin commensal bacteria protect against Staphylococcus aureus and are deficient in atopic dermatitis. Sci Transl Med. 9:pii: eaah46802017. View Article : Google Scholar

7 

De Croo I, Van der Elst J, Everaert K, De Sutter P and Dhont M: Fertilization, pregnancy and embryo implantation rates after ICSI in cases of obstructive and non-obstructive azoospermia. Hum Reprod. 15:1383–1388. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Franasiak JM and Scott RT Jr: Reproductive tract microbiome in assisted reproductive technologies. Fertil Steril. 104:1364–1371. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Javurek AB, Spollen WG, Ali AM, Johnson SA, Lubahn DB, Bivens NJ, Bromert KH, Ellersieck MR, Givan SA and Rosenfeld CS: Discovery of a novel seminal fluid microbiome and influence of estrogen receptor alpha genetic status. Sci Rep. 6:230272016. View Article : Google Scholar : PubMed/NCBI

10 

Tuddenham S and Ghanem KG: A microbiome variable in the HIV-prevention equation. Science. 356:907–908. 2017. View Article : Google Scholar : PubMed/NCBI

11 

De Francesco MA, Negrini R, Ravizzola G, Galli P and Manca N: Bacterial species present in the lower male genital tract: A five-year retrospective study. Eur J Contracept Reprod Health Care. 16:47–53. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Younes JA, Lievens E, Hummelen R, van der Westen R, Reid G and Petrova MI: Women and their microbes: The unexpected friendship. Trends Microbiol. 26:16–32. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Weng SL, Chiu CM, Lin FM, Huang WC, Liang C, Yang T, Yang TL, Liu CY, Wu WY, Chang YA, et al: Bacterial communities in semen from men of infertile couples: Metagenomic sequencing reveals relationships of seminal microbiota to semen quality. PLoS One. 9:e1101522014. View Article : Google Scholar : PubMed/NCBI

14 

Yu X, Wu X, Qiu L, Wang D, Gan M, Chen X, Wei H and Xu F: Analysis of the intestinal microbial community structure of healthy and long-living elderly residents in Gaotian village of liuyang city. Appl Microbiol Biot. 99:9085–9095. 2015. View Article : Google Scholar

15 

Sivan A, Corrales L, Hubert N, Williams JB, Aquino-Michaels K, Earley ZM, Benyamin FW, Lei YM, Jabri B, Alegre ML, et al: Commensal Bifidobacterium promotes antitumor immunity and facilitates anti-PD-L1 efficacy. Science. 350:1084–1089. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Zhernakova A, Kurilshikov A, Bonder MJ, Tigchelaar EF, Schirmer M, Vatanen T, Mujagic Z, Vila AV, Falony G, Vieira-Silva S, et al: Population-based metagenomics analysis reveals markers for gut microbiome composition and diversity. Science. 352:565–569. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Derrien M and van Hylckama Vlieg JE: Fate, activity and impact of ingested bacteria within the human gut microbiota. Trends Microbiol. 1–366. 2015.

18 

Xin F, Xin W, Yang S, Meng F, Wang X, Wei H and Chen T: Evaluation of the microbial diversity in amyotrophic lateral sclerosis using high-throughput sequencing. Front Microbiol. 7:14792016.PubMed/NCBI

19 

Cui M, Xiao H, Li Y, Zhou L, Zhao S, Luo D, Zheng Q, Dong J, Zhao Y, Zhang X, et al: Faecal microbiota transplantation protects against radiation-induced toxicity. EMBO Mol Med. 4:448–461. 2017. View Article : Google Scholar

20 

Chen T, Wu Q, Zhou H, Deng K, Wang X, Meng F, Yang S, Wang X, Shah NP and Wei H: Assessment of commercial probiotic products in China for labelling accuracy and probiotic characterisation of selected isolates. Int J Dairy Technol. 70:119–126. 2017. View Article : Google Scholar

21 

Xu J, Lian F, Zhao L, Zhao Y, Chen X, Zhang X, Guo Y, Zhang C, Zhou Q, Xue Z, et al: Structural modulation of gut microbiota during alleviation of type 2 diabetes with a Chinese herbal formula. Isme J. 9:552–562. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Bolger A, Lohse M and Usadel B: Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics. 30:2114–2120. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Edgar R: UPARSE: Highly accurate OTU sequences from microbial amplicon reads. Nat Methods. 10:996–998. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Steege HT: Measuring biological diversity. Environ Ecol Stat. 1:95–103. 2004.

25 

Cole JR, Wang Q, Fish JA, Chai B, McGarrell DM, Sun Y, Brown CT, Porras-Alfaro A, Kuske CR and Tiedje JM: Ribosomal database project: Data and tools for high throughput rRNA analysis. Nucleic Acids Res. 42(Database Issue): D633–D642. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, et al: QIIME allows analysis of high-throughput community sequencing data. Nat Methods. 7:3352010. View Article : Google Scholar : PubMed/NCBI

27 

Javurek AB, Spollen WG, Johnson SA, Bivens NJ, Bromert KH, Givan SA and Rosenfeld CS: Consumption of a high-fat diet alters the seminal fluid and gut microbiomes in male mice. Reprod Fert Devel. 29:1602–1612. 2017. View Article : Google Scholar

28 

Langille MG, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Vega Thurber RL, Knight R, et al: Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 31:814–821. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Wolf M, Müller T, Dandekar T and Pollack JD: Phylogeny of Firmicutes with special reference to Mycoplasma (Mollicutes) as inferred from phosphoglycerate kinase amino acid sequence data. Int J Syst Evol Microbiol. 54:871–875. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Donders GG, Bosmans E, Dekeersmaeckerb A, Vereecken A, Van Bulck B and Spitz B: Pathogenesis of abnormal vaginal bacterial flora. Am J Obstet Gyneco. 182:872–878. 2000. View Article : Google Scholar

31 

Kovachev SM: Obstetric and gynecological diseases and complications resulting from vaginal. dysbacteriosis. 68:1–184. 2014.

32 

Bae HS, Im WT and Lee ST: Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol. 55:1155–1161. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen H, Luo T, Chen T and Wang G: Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia. Exp Ther Med 15: 2884-2890, 2018.
APA
Chen, H., Luo, T., Chen, T., & Wang, G. (2018). Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia. Experimental and Therapeutic Medicine, 15, 2884-2890. https://doi.org/10.3892/etm.2018.5778
MLA
Chen, H., Luo, T., Chen, T., Wang, G."Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia". Experimental and Therapeutic Medicine 15.3 (2018): 2884-2890.
Chicago
Chen, H., Luo, T., Chen, T., Wang, G."Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2884-2890. https://doi.org/10.3892/etm.2018.5778
Copy and paste a formatted citation
x
Spandidos Publications style
Chen H, Luo T, Chen T and Wang G: Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia. Exp Ther Med 15: 2884-2890, 2018.
APA
Chen, H., Luo, T., Chen, T., & Wang, G. (2018). Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia. Experimental and Therapeutic Medicine, 15, 2884-2890. https://doi.org/10.3892/etm.2018.5778
MLA
Chen, H., Luo, T., Chen, T., Wang, G."Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia". Experimental and Therapeutic Medicine 15.3 (2018): 2884-2890.
Chicago
Chen, H., Luo, T., Chen, T., Wang, G."Seminal bacterial composition in patients with obstructive and non‑obstructive azoospermia". Experimental and Therapeutic Medicine 15, no. 3 (2018): 2884-2890. https://doi.org/10.3892/etm.2018.5778
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team