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Article

Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway

  • Authors:
    • Chanthasone Vongphouttha
    • Jie Zhu
    • Shuhao Deng
    • Wenlin Tai
    • Wenjuan Wu
    • Zhenkun Li
    • Wen Lei
    • Yin Wang
    • Zhaoxing Dong
    • Tao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China, Department of Internal Medicine, Beijing Capital International Airport Hospital, Beijing 100621, P.R. China, Department of Clinical Laboratory, Yunnan Molecular Diagnostic Center, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650101, P.R. China
  • Pages: 3045-3051
    |
    Published online on: January 24, 2018
       https://doi.org/10.3892/etm.2018.5795
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Abstract

Paraquat (PQ) is a herbicide that is widely used in developing countries, and pulmonary fibrosisis one of the most typical features of PQ poisoning. The molecular mechanism underlying PQ toxicity is largely unknown, which makes it difficult to treat. In the present study, western blot analysis, reverse transcription‑quantitative polymerase chain reaction and fluorescent immunostaining were used to analyze the effects of rapamycin on PQ‑induced epithelial‑mesenchymal transition (EMT) in A549 and MRC‑5 cells. It was revealed that rapamycin significantly downregulated the mesenchymal cell marker, α‑smooth muscle actin, and significantly upregulated the epithelial cell marker, E‑cadherin, at mRNA and protein expression levels compared with the PQ group. Treatment with PQ significantly increased Wnt1, low‑density lipoprotein receptor‑related protein (LRP)5, LRP6 and β‑catenin expression levels in A549 cells, while rapamycin significantly inhibited these effects of PQ. Activation of the Wnt signaling pathway using lithium chloride attenuated the inhibitory effects of rapamycin on PQ‑induced EMT. In conclusion, rapamycin protects against PQ‑induced pulmonary EMT via the Wnt/β‑catenin signaling pathway.
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1 

Weng CH, Chen HH, Hu CC, Huang WH, Hsu CW, Fu JF, Lin WR, Wang IK and Yen TH: Predictors of acute kidney injury after paraquat intoxication. Oncotarget. 8:51345–51354. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Sabzghabaee AM, Eizadi-Mood N, Montazeri K, Yaraghi A and Golabi M: Fatality in paraquat poisoning. Singapore Med J. 51:496–500. 2010.PubMed/NCBI

3 

Li T, Yang X, Xin S, Cao Y and Wang N: Paraquat poisoning induced pulmonary epithelial mesenchymal transition through Notch1 pathway. Sci Rep. 7:9242017. View Article : Google Scholar : PubMed/NCBI

4 

Suntres ZE: Role of antioxidants in paraquat toxicity. Toxicology. 180:65–77. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Willis BC and Borok Z: TGF-beta-induced EMT: Mechanisms and implications for fibrotic lung disease. Am J Physiol Lung Cell Mol Physiol. 293:L525–L534. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Li H, Hao Y, Zhang H, Ying W, Li D, Ge Y, Ying B, Cheng B, Lian Q and Jin S: Posttreatment with Protectin DX ameliorates bleomycin-induced pulmonary fibrosis and lung dysfunction in mice. Sci Rep. 7:467542017. View Article : Google Scholar : PubMed/NCBI

7 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Xia MY, Zhao XY, Huang QL, Sun HY, Sun C, Yuan J, He C, Sun Y, Huang X, Kong W and Kong WJ: Activation of Wnt/β-catenin signaling by lithium chloride attenuates d-galactose-induced neurodegeneration in the auditory cortex of a rat model of aging. FEBS Open Bio. 7:759–776. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Yang X, Tang S, Dai C, Li D, Zhang S, Deng S, Zhou Y and Xiao X: Quinocetone induces mitochondrial apoptosis in HepG2 cells through ROS-dependent promotion of VDAC1 oligomerization and suppression of Wnt1/β-catenin signaling pathway. Food Chem Toxicol. 105:161–176. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Lucarini L, Durante M, Lanzi C, Pini A, Boccalini G, Calosi L, Moroni F, Masini E and Mannaioni G: HYDAMTIQ, a selective PARP-1 inhibitor, improves bleomycin-induced lung fibrosis by dampening the TGF-β/SMAD signalling pathway. J Cell Mol Med. 21:324–335. 2017. View Article : Google Scholar : PubMed/NCBI

11 

Wuyts WA, Agostini C, Antoniou KM, Bouros D, Chambers RC, Cottin V, Egan JJ, Lambrecht BN, Lories R, Parfrey H, et al: The pathogenesis of pulmonary fibrosis: A moving target. Eur Respir J. 41:1207–1218. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Borensztajn K, Crestani B and Kolb M: Idiopathic pulmonary fibrosis: From epithelial injury to biomarkers-insights from the bench side. Respiration. 86:441–452. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Kim KK, Kugler MC, Wolters PJ, Robillard L, Galvez MG, Brumwell AN, Sheppard D and Chapman HA: Alveolar epithelial cell mesenchymal transition develops in vivo during pulmonary fibrosis and is regulated by the extracellular matrix. Proc Natl Acad Sci USA. 103:pp. 13180–13185. 2006; View Article : Google Scholar : PubMed/NCBI

14 

Yamada A, Aki T, Unuma K, Funakoshi T and Uemura K: Paraquat induces epithelial-mesenchymal transition-like cellular response resulting in fibrogenesis and the prevention of apoptosis in human pulmonary epithelial cells. PLoS One. 10:e01201922015. View Article : Google Scholar : PubMed/NCBI

15 

Han YY, Shen P and Chang WX: Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-β/Smad signaling in paraquat-induced pulmonary fibrosis. Mol Med Rep. 12:7979–7984. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Xie L, Zhou D, Xiong J, You J, Zeng Y and Peng L: Paraquat induce pulmonary epithelial-mesenchymal transition through transforming growth factor-β1-dependent mechanism. Exp Toxicol Pathol. 68:69–76. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Wang J, Zhu Y, Tan J, Meng X, Xie H and Wang R: Lysyl oxidase promotes epithelial-to-mesenchymal transition during paraquat-induced pulmonary fibrosis. Mol Biosyst. 12:499–507. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Zhu Y, Tan J, Xie H, Wang J, Meng X and Wang R: HIF-1α regulates EMT via the Snail and β-catenin pathways in paraquat poisoning-induced early pulmonary fibrosis. J Cell Mol Med. 20:688–697. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Yang W, Zhao X, Liang R and Chen D: Effects of small RNA interference targeting mammalian target of rapamycin on paraquat-induced pulmonary fibrosis in rats. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 29:830–835. 2017.(In Chinese). PubMed/NCBI

20 

Chen LM, Song TJ, Xiao JH, Huang ZH, Li Y and Lin TY: Tripchlorolide induces autophagy in lung cancer cells by inhibiting the PI3K/AKT/mTOR pathway and improves cisplatin sensitivity in A549/DDP cells. Oncotarget. 8:63911–63922. 2017.PubMed/NCBI

21 

Song Y, Zhang P, Sun Y, Li X, Chen L, Xiao Y and Xing Y: AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells. Oncotarget. 8:67942–67954. 2017.PubMed/NCBI

22 

Chung EJ, Sowers A, Thetford A, McKay-Corkum G, Chung SI, Mitchell JB and Citrin DE: Mammalian target of rapamycin inhibition with rapamycin mitigates radiation-induced pulmonary fibrosis in a murine model. Int J Radiat Oncol Biol Phys. 96:857–866. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Mercer PF, Woodcock HV, Eley JD, Platé M, Sulikowski MG, Durrenberger PF, Franklin L, Nanthakumar CB, Man Y, Genovese F, et al: Exploration of a potent PI3 kinase/mTOR inhibitor as a novel anti-fibrotic agent in IPF. Thorax. 71:701–711. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Xu X, Dai H, Geng J, Wan X, Huang X, Li F, Jiang D and Wang C: Rapamycin increases CCN2 expression of lung fibroblasts via phosphoinositide 3-kinase. Lab Invest. 95:846–859. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Xu Y, Tai W, Qu X, Wu W, Li Z, Deng S, Vongphouttha C and Dong Z: Rapamycin protects against paraquat-induced pulmonary fibrosis: Activation of Nrf2 signaling pathway. Biochem Biophys Res Commun. 490:535–540. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Pozharskaya V, Torres-Gonzalez E, Rojas M, Gal A, Amin M, Dollard S, Roman J, Stecenko AA and Mora AL: Twist: A regulator of epithelial-mesenchymal transition in lung fibrosis. PLoS One. 4:e75592009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Vongphouttha C, Zhu J, Deng S, Tai W, Wu W, Li Z, Lei W, Wang Y, Dong Z, Zhang T, Zhang T, et al: Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway. Exp Ther Med 15: 3045-3051, 2018.
APA
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z. ... Zhang, T. (2018). Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 15, 3045-3051. https://doi.org/10.3892/etm.2018.5795
MLA
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z., Lei, W., Wang, Y., Dong, Z., Zhang, T."Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 15.3 (2018): 3045-3051.
Chicago
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z., Lei, W., Wang, Y., Dong, Z., Zhang, T."Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 3045-3051. https://doi.org/10.3892/etm.2018.5795
Copy and paste a formatted citation
x
Spandidos Publications style
Vongphouttha C, Zhu J, Deng S, Tai W, Wu W, Li Z, Lei W, Wang Y, Dong Z, Zhang T, Zhang T, et al: Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway. Exp Ther Med 15: 3045-3051, 2018.
APA
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z. ... Zhang, T. (2018). Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway. Experimental and Therapeutic Medicine, 15, 3045-3051. https://doi.org/10.3892/etm.2018.5795
MLA
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z., Lei, W., Wang, Y., Dong, Z., Zhang, T."Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 15.3 (2018): 3045-3051.
Chicago
Vongphouttha, C., Zhu, J., Deng, S., Tai, W., Wu, W., Li, Z., Lei, W., Wang, Y., Dong, Z., Zhang, T."Rapamycin protects against paraquat‑induced pulmonary epithelial‑mesenchymal transition via the Wnt/β‑catenin signaling pathway". Experimental and Therapeutic Medicine 15, no. 3 (2018): 3045-3051. https://doi.org/10.3892/etm.2018.5795
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