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Article

Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury

Corrigendum in: /10.3892/etm.2018.6361
  • Authors:
    • Yi‑Pa Sai
    • Yuan‑Chun Song
    • Xing‑Xing Chen
    • Xuan Luo
    • Jing Liu
    • Wei‑Jing Cui
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Gansu Province People's Hospital, Lanzhou, Gansu 730000, P.R. China
  • Pages: 4485-4490
    |
    Published online on: March 6, 2018
       https://doi.org/10.3892/etm.2018.5933
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Abstract

Nephrotic syndrome (NS) is the most common kidney disease in clinical practice and may lead to end‑stage renal failure. Astragalosides (AST) have been clinically tested for the treatment of NS, but their mechanism of action has remained to be elucidated. The aim of the present study was to investigate the effect of AST on the structure and function of podocytes with adriamycin (ADR)‑induced damage and to elucidate the underlying molecular mechanisms. The mouse podocyte clone 5 (MPC5) immortalized mouse podocyte cell line was treated with 0.5 µmol/l ADR to establish a podocyte injury model. The MPC5 podocytes were divided into a control group, a podocyte injury group and a low‑, medium‑ and high-concentration AST treatment group. The results indicated that the survival rate of the podocyte injury group was significantly decreased compared with that in the control group and each AST‑treated group had an increased survival rate compared with that in the podocyte injury group. Furthermore, each dose of AST significantly inhibited the ADR-associated increases the levels of lactate dehydrogenase and malondialdehyde and the decrease in the activity of superoxide dismutase in MPC5 podocytes. In addition, AST improved the migration ability of MPC5 podocytes and suppressed the cytoskeletal rearrangement associated with ADR‑induced damage. Furthermore, matrix metalloproteinase (MMP)-2 and ‑9 were decreased in the podocyte injury group, which was inhibited by different concentrations of AST. Thus, AST was able to maintain the balance of oxidative stress in podocytes cultured with ADR and protect them from ADR-induced injury. The mechanism may be associated with the upregulation of MMPs.
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Copy and paste a formatted citation
Spandidos Publications style
Sai YP, Song YC, Chen XX, Luo X, Liu J and Cui WJ: Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361. Exp Ther Med 15: 4485-4490, 2018.
APA
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., & Cui, W. (2018). Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361. Experimental and Therapeutic Medicine, 15, 4485-4490. https://doi.org/10.3892/etm.2018.5933
MLA
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., Cui, W."Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361". Experimental and Therapeutic Medicine 15.5 (2018): 4485-4490.
Chicago
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., Cui, W."Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4485-4490. https://doi.org/10.3892/etm.2018.5933
Copy and paste a formatted citation
x
Spandidos Publications style
Sai YP, Song YC, Chen XX, Luo X, Liu J and Cui WJ: Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361. Exp Ther Med 15: 4485-4490, 2018.
APA
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., & Cui, W. (2018). Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361. Experimental and Therapeutic Medicine, 15, 4485-4490. https://doi.org/10.3892/etm.2018.5933
MLA
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., Cui, W."Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361". Experimental and Therapeutic Medicine 15.5 (2018): 4485-4490.
Chicago
Sai, Y., Song, Y., Chen, X., Luo, X., Liu, J., Cui, W."Protective effect of astragalosides from Radix Astragali on adriamycin-induced podocyte injury Corrigendum in /10.3892/etm.2018.6361". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4485-4490. https://doi.org/10.3892/etm.2018.5933
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