Identification of long non-coding RNA and mRNA expression in βΒ2-crystallin knockout mice

Jia,Yin; Xiong,Kang; Ren,Han‑Xiao; Li,Wen‑Jie

doi:10.3892/etm.2018.5949

Identification of long non-coding RNA and mRNA expression in βΒ2-crystallin knockout mice

Authors:
- Yin Jia
- Kang Xiong
- Han‑Xiao Ren
- Wen‑Jie Li
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Affiliations: Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China
Published online on: March 12, 2018 https://doi.org/10.3892/etm.2018.5949
Pages: 4277-4283
Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

βΒ2-crystallin (CRYBB2) is expressed at an increased level in the postnatal lens cortex and is associated with cataracts. Improved understanding of the underlying biology of cataracts is likely to be critical for the development of early detection strategies and new therapeutics. The present study aimed to identify long non‑coding RNAs (lncRNAs) and mRNAs associated with CRYBB2 knockdown (KO)‑induced cataracts. RNAs from 3 non‑treated mice and 3 CRYBB2 KO mice were analyzed using the Affymetrix GeneChip Mouse Gene 2.0 ST array. A total of 149 lncRNAs and 803 mRNAs were identified to have upregulated expression, including Snora73b, Klk1b22 and Rnu3a, while the expression levels of 180 lncRNAs and 732 mRNAs were downregulated in CRYBB2 KO mice, including Snord82, Snhg9 and Foxn3. This lncRNA and mRNA expression profile of mice with CRYBB2 KO provides a basis for studying the genetic mechanisms of cataract progression.

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