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Sevoflurane-induced memory impairment in the postnatal developing mouse brain

  • Authors:
    • Zhijun Lu
    • Jihui Sun
    • Yichun Xin
    • Ken Chen
    • Wen Ding
    • Yujia Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China, Intensive Care Unit, Shanghai Jing'an District Shibei Hospital, Shanghai 200443, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4097-4104
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    Published online on: March 12, 2018
       https://doi.org/10.3892/etm.2018.5950
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Abstract

The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase‑3 by immunohistochemistry, cleavage of poly adenosine diphosphate‑ribose polymerase (PARP) by western blotting, as well as levels of brain‑derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro‑BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme‑linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase‑3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro‑BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase‑3 induced cleavage of PARP, as well as pro‑BDNF, p75NTR and PKB/Akt may be important in sevoflurane‑induced memory impairment in the postnatal developing mouse brain.
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Copy and paste a formatted citation
Spandidos Publications style
Lu Z, Sun J, Xin Y, Chen K, Ding W and Wang Y: Sevoflurane-induced memory impairment in the postnatal developing mouse brain. Exp Ther Med 15: 4097-4104, 2018.
APA
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., & Wang, Y. (2018). Sevoflurane-induced memory impairment in the postnatal developing mouse brain. Experimental and Therapeutic Medicine, 15, 4097-4104. https://doi.org/10.3892/etm.2018.5950
MLA
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., Wang, Y."Sevoflurane-induced memory impairment in the postnatal developing mouse brain". Experimental and Therapeutic Medicine 15.5 (2018): 4097-4104.
Chicago
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., Wang, Y."Sevoflurane-induced memory impairment in the postnatal developing mouse brain". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4097-4104. https://doi.org/10.3892/etm.2018.5950
Copy and paste a formatted citation
x
Spandidos Publications style
Lu Z, Sun J, Xin Y, Chen K, Ding W and Wang Y: Sevoflurane-induced memory impairment in the postnatal developing mouse brain. Exp Ther Med 15: 4097-4104, 2018.
APA
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., & Wang, Y. (2018). Sevoflurane-induced memory impairment in the postnatal developing mouse brain. Experimental and Therapeutic Medicine, 15, 4097-4104. https://doi.org/10.3892/etm.2018.5950
MLA
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., Wang, Y."Sevoflurane-induced memory impairment in the postnatal developing mouse brain". Experimental and Therapeutic Medicine 15.5 (2018): 4097-4104.
Chicago
Lu, Z., Sun, J., Xin, Y., Chen, K., Ding, W., Wang, Y."Sevoflurane-induced memory impairment in the postnatal developing mouse brain". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4097-4104. https://doi.org/10.3892/etm.2018.5950
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