Effects of cisatracurium in combination with ventilation on inflammatory factors and immune variations in sepsis rats

He,Tongyun; Tao,Jianmei; Wang,Xinqiang; Wang,Xuena

doi:10.3892/etm.2018.5978

Effects of cisatracurium in combination with ventilation on inflammatory factors and immune variations in sepsis rats

Authors:
- Tongyun He
- Jianmei Tao
- Xinqiang Wang
- Xuena Wang
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Affiliations: Department of Anesthesiology, The First People's Hospital of Huzhou, Huzhou, Zhejiang 313000, P.R. China, Department of Pharmacy, The First People's Hospital of Huzhou, Huzhou, Zhejiang 313000, P.R. China
Published online on: March 20, 2018 https://doi.org/10.3892/etm.2018.5978
Pages: 4414-4418
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of cisatracurium in combination with ventilation on inflammatory factors and immune variations in sepsis rats were investigated. A total of 54 male Sprague‑Dawley rats were selected and divided randomly into three groups: Sham group (n=6), model group (n=24) and experiment group (n=24). Rats in the model and experiment groups underwent cecal ligation and puncture (CLP) for establishment of sepsis model. Rats in experiment group additionally received cisatracurium medication in combination with ventilation for treatment. At 6, 12 and 24 h after CLP, the levels of tumor necrosis factor (TNF)-α, interleukin (IL)‑1β, IL-6 and procalcitonin (PCT) in serum and the ratio of leukocyte to neutrophil in peripheral blood was also detected. Twenty-four hours later, the expression of high mobility group box 1 (HMGB1) in lung tissues and cluster of differentiation (CD) 4+ and CD8+ in T-lymphocyte subsets were also detected, and the wet/dry (W/D) ratio of lung was measured. Compared with that in model group, the levels of inflammatory factors in the experiment group were significantly decreased, while the indicators in assays of cellular immunity were obviously elevated. Ratio of leukocyte to neutrophil in peripheral blood was significantly decreased after treatment. Cisatracurium in combination with ventilation can alleviate the inflammatory injury to organs in sepsis rats through inhibiting the inflammatory responses and regulating the immune functions, which manifests a new significance in guiding the clinical diagnosis and treatment.

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