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Article Open Access

Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance

  • Authors:
    • Jing Tong
    • Xi‑Jiang Hu
    • Wen‑Qian Cai
    • Xiang Dai
    • Lei Wang
  • View Affiliations / Copyright

    Affiliations: Aristogenesis Genetic Laboratory, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430015, P.R. China, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China
    Copyright: © Tong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4441-4447
    |
    Published online on: March 22, 2018
       https://doi.org/10.3892/etm.2018.5990
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Abstract

Puerarin has long been used as a traditional Chinese medicine, which possesses various physiological properties, including anti‑inflammation, anti‑oxidative, anti‑diabetic and anti‑cancer activities. The aim of the current study was to investigate the effect of puerarin on delayed‑type hypersensitivity (DTH) induced by ovalbumin (OVA) in mice and explore its underlying mechanisms. The results showed that puerarin significantly attenuated DTH, resulting from a decrement in footpad swelling, reduction in inflammatory cell as well as a decline in anti‑OVA IgG in serum. In the homogenized supernatant of footpad tissues, the classic Th1‑cytokines, including interferon (IFN)‑γ was suppressed following puerarin treatment. Furthermore, a high dose of puerarin inhibited interleukin (IL)‑4 production, the classic Th2‑cytokine. The concanavalin A stimulation and MTT assays indicated a suppressive effect of puerarin on Th1 response via decreasing IFN‑γ production in OVA‑primed lymphocytes. Detailed studies revealed that puerarin modulated the Th1/Th2 balance in DTH responses, attributing to lower T‑bet/GATA binding protein‑3 mRNA and protein level ratios, which led to the shift change of IFN‑γ/IL‑4 with puerarin treatment. These findings demonstrate that puerarin alleviated inflammation in DTH triggered by OVA application via curbing inflammatory cytokines by modulating the Th1/Th2 balance. These results suggest that puerarin may be an alternative therapeutic option for the treatment of DTH.
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1 

Abbas AK, Murphy KM and Sher A: Functional diversity of helper T lymphocytes. Nature. 383:787–793. 1996. View Article : Google Scholar : PubMed/NCBI

2 

Neurath MF, Finotto S and Glimcher LH: The role of Th1/Th2 polarization in mucosal immunity. Nat Med. 8:567–573. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Kidd P: Th1/Th2 balance: The hypothesis, its limitations, and implications for health and disease. Altern Med Rev. 8:223–246. 2003.PubMed/NCBI

4 

Kobayashi K, Kaneda K and Kasama T: Immunopathogenesis of delayed-type hypersensitivity. Microsc Res Tech. 53:241–245. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Hernández A, Yager JA, Wilkie BN, Leslie KE and Mallard BA: Evaluation of bovine cutaneous delayed-type hypersensitivity (DTH) to various test antigens and a mitogen using several adjuvants. Vet Immunol Immunopathol. 104:45–58. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Jacysyn JF, Abrahamsohn IA and Macedo MS: Modulation of delayed-type hypersensitivity during the time course of immune response to a protein antigen. Immunology. 102:373–379. 2001. View Article : Google Scholar : PubMed/NCBI

7 

Zhou YX, Zhang H and Peng C: Puerarin: A review of pharmacological effects. Phytother Res. 28:961–975. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Zhang Z, Lam TN and Zuo Z: Radix Puerariae: An overview of its chemistry, pharmacology, pharmacokinetics and clinical use. J Clin Pharmacol. 53:787–811. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Liu XJ, Zhao J and Gu XY: The effects of genistein and puerarin on the activation of nuclear factor-kappaB and the production of tumor necrosis factor-alpha in asthma patients. Pharmazie. 65:127–131. 2010.PubMed/NCBI

10 

Wang J, Zhang T, Ma C and Wang S: Puerarin attenuates airway inflammation by regulation of eotaxin-3. Immunol Lett. 163:173–178. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Mahdy HM, Mohamed MR, Emam MA, Karim AM, Abdel-Naim AB and Khalifa AE: The anti-apoptotic and anti-inflammatory properties of puerarin attenuate 3-nitropropionic-acid induced neurotoxicity in rats. Can J Physiol Pharmacol. 92:252–258. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Zhang D, Ma G, Hou M, Zhang T, Chen L and Zhao C: The Neuroprotective effect of Puerarin in acute spinal cord injury rats. Cell Physiol Biochem. 39:1152–1164. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Wang Z, Ouyang L, Liang Z, Chen J, Yu Q, Jeza VT, Gong Y, Shen G, Weng X and Wu X: CD8(low)CD28(-) T cells: A human CD8 T-suppressor subpopulation with alloantigen specificity induced by soluble HLA-A2 dimer in vitro. Cell Transplant. 24:2129–2142. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Yu Q, Zhang L, Ouyang L, Gong Y, Liang Z, Shen G, Weng X and Wu X: A similarity in peptide cross-reactivity between alloantigen- and nominal antigen-induced CD8+ T cell responses in vitro. Immunogenetics. 65:173–184. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Weng X, Lu S, Zhong M, Liang Z, Shen G, Chen J and Wu X: Allo-restricted CTLs generated by coculturing of PBLs and autologous monocytes loaded with allogeneic peptide/HLA/IgG1-Fc fusion protein. J Leukoc Biol. 85:574–581. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Zhang X, Xiong J, Liu S, Wang L, Huang J, Liu L, Yang J, Zhang G, Guo K, Zhang Z, et al: Puerarin protects dopaminergic neurons in Parkinson's disease models. Neuroscience. 280:88–98. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Wang C, Wang W, Jin X, Shen J, Hu W and Jiang T: Puerarin attenuates inflammation and oxidation in mice with collagen antibody-induced arthritis via TLR4/NF-κB signaling. Mol Med Rep. 14:1365–1370. 2016. View Article : Google Scholar : PubMed/NCBI

18 

Yuan Y, Zhou H, Wu QQ, Li FF, Bian ZY, Deng W, Zhou MQ and Tang QZ: Puerarin attenuates the inflammatory response and apoptosis in LPS-stimulated cardiomyocytes. Exp Ther Med. 11:415–420. 2016. View Article : Google Scholar : PubMed/NCBI

19 

Raundhal M, Morse C, Khare A, Oriss TB, Milosevic J, Trudeau J, Huff R, Pilewski J, Holguin F, Kolls J, et al: High IFN-γ and low SLPI mark severe asthma in mice and humans. J Clin Invest. 125:3037–3050. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Wang Z and Chen Z, Chen T, Yi T, Zheng Z, Fan H and Chen Z: Berberine attenuates inflammation associated with delayed-type hypersensitivity via suppressing Th1 response and inhibiting apoptosis. Inflammation. 40:221–231. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Hirose K, Iwata A, Tamachi T and Nakajima H: Allergic airway inflammation: Key players beyond the Th2 cell pathway. Immunol Rev. 278:145–161. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Ashraf MI, Shahzad M and Shabbir A: Oxyresveratrol ameliorates allergic airway inflammation via attenuation of IL-4, IL-5 and IL-13 expression levels. Cytokine. 76:375–381. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Hirahara K and Nakayama T: CD4+ T-cell subsets in inflammatory diseases: Beyond the Th1/Th2 paradigm. Int Immunol. 28:163–171. 2016. View Article : Google Scholar : PubMed/NCBI

24 

Bui TT, Piao CH, Kim SM, Song CH, Shin HS, Lee CH and Chai OH: Citrus tachibana leaves ethanol extract alleviates airway inflammation by the modulation of Th1/Th2 imbalance via inhibiting NF-κB signaling and histamine secretion in a mouse model of allergic asthma. J Med Food. 20:676–684. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Peine M, Rausch S, Helmstetter C, Fröhlich A, Hegazy AN, Kühl AA, Grevelding CG, Höfer T, Hartmann S and Löhning M: Stable T-bet(+) GATA-3(+) Th1/Th2 hybrid cells arise in vivo, can develop directly from naive precursors and limit immunopathologic inflammation. PLoS Biol. 11:e10016332013. View Article : Google Scholar : PubMed/NCBI

26 

Yagi R, Zhu J and Paul WE: An updated view on transcription factor GATA3-mediated regulation of Th1 and Th2 cell differentiation. Int Immunol. 23:415–420. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Tong J, Hu XJ, Cai WQ, Dai X and Wang L: Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Exp Ther Med 15: 4441-4447, 2018.
APA
Tong, J., Hu, X., Cai, W., Dai, X., & Wang, L. (2018). Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Experimental and Therapeutic Medicine, 15, 4441-4447. https://doi.org/10.3892/etm.2018.5990
MLA
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15.5 (2018): 4441-4447.
Chicago
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4441-4447. https://doi.org/10.3892/etm.2018.5990
Copy and paste a formatted citation
x
Spandidos Publications style
Tong J, Hu XJ, Cai WQ, Dai X and Wang L: Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Exp Ther Med 15: 4441-4447, 2018.
APA
Tong, J., Hu, X., Cai, W., Dai, X., & Wang, L. (2018). Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance. Experimental and Therapeutic Medicine, 15, 4441-4447. https://doi.org/10.3892/etm.2018.5990
MLA
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15.5 (2018): 4441-4447.
Chicago
Tong, J., Hu, X., Cai, W., Dai, X., Wang, L."Puerarin alleviates delayed-type hypersensitivity via cytokine inhibition by modulating Th1/Th2 balance". Experimental and Therapeutic Medicine 15, no. 5 (2018): 4441-4447. https://doi.org/10.3892/etm.2018.5990
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