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Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis

  • Authors:
    • Yunbo Ma
    • Yongxia Zhang
    • Yanyan Zhao
    • Xianhua Wang
    • Yuefu Lin
    • Aiguo Ma
  • View Affiliations / Copyright

    Affiliations: Department of Preventive Medicine, Linyi People's Hospital, Linyi, Shandong 276034, P.R. China, Department of Emergency, Linyi People's Hospital, Linyi, Shandong 276034, P.R. China, Department of Clinical Laboratory, Linyi People's Hospital, Linyi, Shandong 276034, P.R. China, School of Public Health, Qingdao University, Qingdao, Shandong 266021, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4671-4676
    |
    Published online on: March 28, 2018
       https://doi.org/10.3892/etm.2018.5999
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Abstract

The expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis (TBM) and their clinical significance in patients with TBM was investigated. Sixty patients with TBM (observation group) and twenty healthy volunteers during the same period (control group) were selected and the cerebrospinal fluid was collected. The expression levels of p62, Beclin1 and LC3-II genes in cerebrospinal fluid were detected via semi‑quantitative reverse transcription-polymerase chain reaction and patients in observation group were divided into high expression and normal or low expression group on the basis of LC3-II expression levels. On the other hand, the contents of inflammatory factors interleukin-6, -10 (IL-6, -10), and tumor necrosis factor-α (TNF-α) were detected using the enzyme-linked immunosorbent assay kit. The mRNA levels of p62, Beclin1 and LC3-II in cerebrospinal fluid of patients in observation were significantly higher than those in the control group (P<0.01). TUNEL assay showed that the apoptosis level of cerebro-spinal fluid in high expression was obviously lower than that in normal or low expression group (P<0.01); the content of IL-6 and TNF-α in cerebrospinal fluid in high expression was significantly lower than those in normal or low expression group (P<0.01); the content of IL-10 in cerebrospinal fluid in high expression was obviously higher than that in normal or low expression group (P<0.01). Correlation analysis revealed that LC3-II was positively correlated with IL-10, but negatively correlated with IL-6 and TNF-α. The mRNA levels of p62, Beclin1 and LC3-II in cerebrospinal fluid of patients with TBM are increased, there is a correlation between expression levels of autophagy-related genes and inflammatory factors, and the high expression of autophagy-related genes may have a protective effect on patients with TBM.
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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Zhang Y, Zhao Y, Wang X, Lin Y and Ma A: Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis. Exp Ther Med 15: 4671-4676, 2018.
APA
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., & Ma, A. (2018). Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis. Experimental and Therapeutic Medicine, 15, 4671-4676. https://doi.org/10.3892/etm.2018.5999
MLA
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., Ma, A."Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis". Experimental and Therapeutic Medicine 15.6 (2018): 4671-4676.
Chicago
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., Ma, A."Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4671-4676. https://doi.org/10.3892/etm.2018.5999
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Zhang Y, Zhao Y, Wang X, Lin Y and Ma A: Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis. Exp Ther Med 15: 4671-4676, 2018.
APA
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., & Ma, A. (2018). Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis. Experimental and Therapeutic Medicine, 15, 4671-4676. https://doi.org/10.3892/etm.2018.5999
MLA
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., Ma, A."Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis". Experimental and Therapeutic Medicine 15.6 (2018): 4671-4676.
Chicago
Ma, Y., Zhang, Y., Zhao, Y., Wang, X., Lin, Y., Ma, A."Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4671-4676. https://doi.org/10.3892/etm.2018.5999
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