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Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis

  • Authors:
    • Song Hong
    • Yi Liu
    • Huazhang Xiong
    • Dongfeng Cai
    • Qinghong Fan
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Affiliated Hospital of Zunyi Medical College, Zunyi, Guizhou 563003, P.R. China
    Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4925-4931
    |
    Published online on: April 3, 2018
       https://doi.org/10.3892/etm.2018.6031
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Abstract

Eukaryotic translation initiation factor 3H subunit (EIF3H) is a member of the EIF3 family and exhibits a central role in translation initiation in higher eukaryotes. Although EIF3H expression is upregulated in numerous tumour types, its potential role in human osteosarcoma (OS) has not yet been investigated. In the present study, it was demonstrated that EIF3H mRNA expression was upregulated in the human OS cell lines Saos‑2 and U2OS. A recombinant lentivirus harbouring short hairpin RNA targeting EIF3H was constructed and successfully infected human OS Saos‑2 and U2OS cells, resulting in 95% downregulated EIF3H expression compared with the respective control groups. Knockdown of EIF3H significantly inhibited the proliferation and colony formation of OS cells in vitro, and tumour growth in nude mice in vivo. Flow cytometry analysis revealed cell cycle arrest and promotion of apoptosis in OS cells with EIF3H knocked down. In conclusion, the results strongly suggested that EIF3H is a critical factor mediating the growth of OS cells and may represent a novel therapeutic target.
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Copy and paste a formatted citation
Spandidos Publications style
Hong S, Liu Y, Xiong H, Cai D and Fan Q: Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis. Exp Ther Med 15: 4925-4931, 2018.
APA
Hong, S., Liu, Y., Xiong, H., Cai, D., & Fan, Q. (2018). Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis. Experimental and Therapeutic Medicine, 15, 4925-4931. https://doi.org/10.3892/etm.2018.6031
MLA
Hong, S., Liu, Y., Xiong, H., Cai, D., Fan, Q."Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis". Experimental and Therapeutic Medicine 15.6 (2018): 4925-4931.
Chicago
Hong, S., Liu, Y., Xiong, H., Cai, D., Fan, Q."Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4925-4931. https://doi.org/10.3892/etm.2018.6031
Copy and paste a formatted citation
x
Spandidos Publications style
Hong S, Liu Y, Xiong H, Cai D and Fan Q: Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis. Exp Ther Med 15: 4925-4931, 2018.
APA
Hong, S., Liu, Y., Xiong, H., Cai, D., & Fan, Q. (2018). Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis. Experimental and Therapeutic Medicine, 15, 4925-4931. https://doi.org/10.3892/etm.2018.6031
MLA
Hong, S., Liu, Y., Xiong, H., Cai, D., Fan, Q."Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis". Experimental and Therapeutic Medicine 15.6 (2018): 4925-4931.
Chicago
Hong, S., Liu, Y., Xiong, H., Cai, D., Fan, Q."Eukaryotic translation initiation factor 3H suppression inhibits osteocarcinoma cell growth and tumorigenesis". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4925-4931. https://doi.org/10.3892/etm.2018.6031
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