Open Access

Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia

  • Authors:
    • Xiaolei Sun
    • Jie Sun
    • Xiaoyi Shao
    • Jinrong Feng
    • Junming Yan
    • Yongwei Qin
  • View Affiliations

  • Published online on: April 3, 2018     https://doi.org/10.3892/etm.2018.6032
  • Pages: 4709-4716
  • Copyright : © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

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Abstract

Endotoxin tolerance is an immunohomeostatic reaction to reiterant lipopolysaccharide (LPS) exposure that maintains a state of altered responsiveness in immune cells, resulting in the inhibition of the pro‑inflammatory response and the resolution of inflammation. Microglia constitutes the first line of defense against endogenous and external challenges in the brain. MicroRNAs (miRs) serve a critical function in the regulation of inflammation. The aim of the present study was to investigate whether miR‑155 regulates endotoxin tolerance. miR‑155 and suppressor of cytokine signaling‑1 (SOCS1) mRNA expression was measured using RT‑qPCR. The expression of SOCS1 was measured by western blotting and immunofluorescence. TNF‑α levels were detected by an enzyme‑linked immunosorbent assay. The results indicated that miR‑155 expression was significantly downregulated in the microglia and cortex tissue following the induction of endotoxin tolerance. This was consistent with an increase in the expression of SOCS1, a predicted target of miR‑155 and key inhibitor of the inflammatory reaction. Transfection with miR‑155 inhibitor significantly enhanced SOCS1 expression in the microglia following the induction of endotoxin tolerance. SOCS1 knockdown using short hairpin RNA partly inhibited the anti‑inflammatory process and promoted the inflammatory response during endotoxin tolerance. The results of the current study indicate that miR‑155 inhibition contributes to the development of endotoxin tolerance. Understanding how miRs regulate inflammatory mechanisms may facilitate the development of novel therapeutic strategies to treat CNS disorders.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Sun X, Sun J, Shao X, Feng J, Yan J and Qin Y: Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia. Exp Ther Med 15: 4709-4716, 2018
APA
Sun, X., Sun, J., Shao, X., Feng, J., Yan, J., & Qin, Y. (2018). Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia. Experimental and Therapeutic Medicine, 15, 4709-4716. https://doi.org/10.3892/etm.2018.6032
MLA
Sun, X., Sun, J., Shao, X., Feng, J., Yan, J., Qin, Y."Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia". Experimental and Therapeutic Medicine 15.6 (2018): 4709-4716.
Chicago
Sun, X., Sun, J., Shao, X., Feng, J., Yan, J., Qin, Y."Inhibition of microRNA-155 modulates endotoxin tolerance by upregulating suppressor of cytokine signaling 1 in microglia". Experimental and Therapeutic Medicine 15, no. 6 (2018): 4709-4716. https://doi.org/10.3892/etm.2018.6032